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Potassium phosphate dibasic

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Potassium phosphate dibasic is a chemical compound with the formula K2HPO4. It is a white, crystalline solid that is commonly used as a buffering agent and pH regulator in various laboratory applications.

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Lab products found in correlation

Potassium phosphate monobasic, by Thermo Fisher Scientific (20 mentions) Methanol, by Thermo Fisher Scientific (4 mentions) D glucose, by Merck Group (3 mentions) Bovine serum albumin (bsa), by Merck Group (3 mentions) Sodium hydroxide (naoh), by Merck Group (2 mentions) Glycine, by Thermo Fisher Scientific (2 mentions) Glucose oxidase from aspergillus niger, by Merck Group (2 mentions) Milli q high purity water unit, by Merck Group (2 mentions) 9 10 phenanthrenequinone pqn, by Thermo Fisher Scientific (2 mentions) 5 5 dithiobis 2 nitrobenzoic acid dtnb, by Thermo Fisher Scientific (2 mentions) Dl dithiothreitol dtt, by Thermo Fisher Scientific (2 mentions) Methanol, by Merck Group (2 mentions) Ascorbic acid, by Merck Group (2 mentions) Chelex 100 sodium form, by Merck Group (2 mentions) Horseradish peroxidase, by Merck Group (2 mentions) Formic acid, by Thermo Fisher Scientific (2 mentions) Milli q, by Merck Group (2 mentions) Sodium cyanoborohydride, by Merck Group (2 mentions) Citric acid, by Thermo Fisher Scientific (2 mentions) Potassium chloride (kcl), by Thermo Fisher Scientific (2 mentions)

Spelling variants of this product

Potassium phosphate (23 citations) Potassium phosphate monobasic and dibasic (6 citations)

29 protocols using potassium phosphate dibasic

1

Quantitative Analysis of Vitamins and Compounds

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Thiamin hydrochloride, thiamin-monophosphate, thiamin-pyrophosphate, riboflavin, flavin adenine dinucleotide, riboflavin-dioxopyrimidine-13C4,15N2, perchloric acid, phosphoric acid and sodium hydroxide were purchased from Sigma-Aldrich (St. Louis, MO). Methanol, acetonitrile, water (all LC-MS grade), phosphoric acid and potassium phosphate dibasic were obtained from Fisher Scientific (Fair Lawn, NJ). Potassium ferricyanide (III) was purchased from Acros Organics (Geel, Belgium); caffeine-trimethyl-13C3 from Cambridge Isotope Laboratories (Andover, MA) and ammonium formate from Hampton Research (Aliso Viejo, CA). HPLC screw-cap amber vials and inserts were obtained from Supelco (Bellefonte, PA). LC-vial caps (PTFE/silicone) were purchased from Waters (Milford, MA) and Agilent Technologies (Santa Clara, CA), and ultrafree centrifugal filters Durapore® PVDF 0.1μm from Millipore (Billerica, MA).
Hampel D., Shahab-Ferdows S., Adair L.S., Bentley M.E., Flax V.L., Jamieson D.J., Ellington S.R., Tegha G., Chasela C.S., Kamwendo D, & Allen L.H. (2016). Thiamin and Riboflavin in Human Milk: Effects of Lipid-Based Nutrient Supplementation and Stage of Lactation on Vitamer Secretion and Contributions to Total Vitamin Content. PLoS ONE, 11(2), e0149479.
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2

Anaerobic Growth of E. coli Strains

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DH1, DH1ΔacrB and BW25113 E. coli strains were inoculated at an OD600 of 0.35-0.45 in 250 mL screw-capped baffled Erlenmeyer flasks in TB (terrific broth) medium (1 L contains 12 g casein peptone, 24 g yeast extract, 4 mL glycerol, 2.2 g potassium phosphate monobasic, 9.4 g potassium phosphate dibasic, Fisher Scientific, Pittburgh, PA, USA) and grown anaerobically at 30 °C and 215 rpm for up to 72 h. DH1ΔacrB strain was constructed by removing the efflux pump subunit acrB from the chromosome according to established procedures [28 (link)].
Bauer S, & Ibáñez A.B. (2015). Does size matter? Separations on guard columns for fast sample analysis applied to bioenergy research. BMC Biotechnology, 15, 38.
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3

Water Treatment Chemical Analysis

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All of the chemicals were of analytical reagent (AR) grade. 4-nitrophenol (99%) was obtained from Alfa Aesar (Tewksbury, MA, USA). Naphthalene (99.7%) was acquired from Mallinckrodt Pharmaceuticals (Blanchardstown, Dublin, Ireland). Potassium phosphate dibasic (98%) and potassium phosphate monobasic (99%) were purchased from Fisher Scientific (New York, NY, USA). Calcium carbonate (99%) was obtained from BDH Chemicals (Mississauga, ON, Canada). All of the stock solutions were prepared using 18 MΩ cm Millipore water (Burlington, MA, USA), unless specified otherwise. Ferric sulfate (60% w/v), lime (10% w/v) solutions, and water samples were obtained from the South Saskatchewan River water samples after primary treatment at the City of Saskatoon Water Treatment Plant (Saskatoon, SK, Canada). Aluminum sulfate (48.5% w/v) was contributed by Chemtrade Logistics (Saskatoon, SK, Canada).
Venegas-García D.J, & Wilson L.D. (2023). Kinetics and Thermodynamics of Adsorption for Aromatic Hydrocarbon Model Systems via a Coagulation Process with a Ferric Sulfate–Lime Softening System. Materials, 16(2), 655.
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4

Protein Purification Protocol

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The following reagents were obtained from Fisher Scientific: glycine, potassium phosphate monobasic, potassium phosphate dibasic, HEPES, and glycerol. A bicinchoninic acid assay kit was from Thermo Scientific. Pyridoxal 5′-phosphate, bovine serum albumin (BSA) standards, DEAE resin, ACA-44 ultrogel, and sodium cyanoborohydride were from Sigma–Aldrich.
Stojanovski B.M, & Ferreira G.C. (2015). Murine erythroid 5-aminolevulinate synthase: Adenosyl-binding site Lys221 modulates substrate binding and catalysis. FEBS Open Bio, 5, 824-831.
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5

Transdermal Niacinamide and PEG 400 Formulation

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Niacinamide and polyethylene glycol 400 (PEG 400) were purchased from Sigma Aldrich (St Louis, MO, USA). PLGA polymers, EXPANSORB® DLG 50-2A (1:1 lactide/glycolide ratio; molecular weight (MW) range: 15–30 kDa) and EXPANSORB® DLG 50-8A (1:1 lactide/glycolide ratio; MW range: 80–130 kDa) were kindly donated by Merck KGaA (Darmstadt, Germany). Dr. Pen™ Ultima A6 (MN device) was purchased from Dr. Pen Inc. (San Jose, CA, USA). Porcine ear skin was obtained from a local slaughterhouse (Atlanta, GA, USA). Dimethyl sulfoxide (DMSO, Procipient®) was obtained as a sample from Gaylord Chemical (Slidell, LA, USA). Fluoresoft® was purchased from Alden Optical (Lancaster, NY, USA). Potassium phosphate dibasic, phosphate-buffered saline 10× solution, HPLC grade acetonitrile, and orthophosphoric acid were purchased from Fisher Scientific (Bridgewater, NJ, USA). HPLC grade methanol was obtained from Medsupply Partners (Atlanta, GA, USA).
Bhattaccharjee S., Beck-Broichsitter M, & Banga A.K. (2020). In Situ Gel Formation in Microporated Skin for Enhanced Topical Delivery of Niacinamide. Pharmaceutics, 12(5), 472.
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6

Enzymatic Electrochemical Assay Design

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2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) diammonium salt, 2′-Azobis(2-methylpropionitrile) (AIBN), calf serum bovine donor, diethyl ether, D-glucose, glucose oxidase from Aspergillus niger, l-glutathione reduced, laccase from Trametes versicolor, and poly(ethylene glycol) diglycidyl ether (PEGDGE, Mn 500) were used as received from Sigma-Aldrich (St. Louis, MO, USA). 1-vinylimidazole was used as received from TCI America. Bilirubin oxidase was kindly provided by Amano Enzyme (Elgin, IL, USA). Tyrosinase from mushrooms was purchased from Calzyme Laboratories Inc. (San Luis Obispo, CA, USA). Acetone, ethanol, and methanol were purchased from Pharmco. Citric acid, potassium phosphate dibasic, and potassium phosphate monobasic were used as received from Fisher Scientific.
Roy B.G., Rutherford J.L., Weaver A.E., Beaver K, & Rasmussen M. (2020). A Self-Powered Biosensor for the Detection of Glutathione. Biosensors, 10(9), 114.
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7

Enzyme Purification and Characterization

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Acrylamide, potassium phosphate monobasic, potassium phosphate dibasic, Tris, glycine, acetylacetone, and sodium acetate trihydrate were purchased from Fisher. PLP, 8-anilino-1-naphtalenesulfonic acid (ANS), p-dimethylaminobenzaldehyde, and CAPS were from Sigma-Aldrich. AMPSO was from MP Biomedicals, and trichloroacetic acid (TCA) and 5-aminolevulinate hydrochloride were from Acros Organics.
Stojanovski B.M., Breydo L., Hunter G.A., Uversky V.N, & Ferreira G.C. (2014). Catalytically active alkaline molten globular enzyme: Effect of pH and temperature on the structural integrity of 5-aminolevulinate synthase*. Biochimica et biophysica acta, 1844(12), 2145-2154.
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8

Electrochemical Biosensor for Metabolite Detection

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Chitosan, bovine serum albumin (BSA), D- (+) glucose, glucose oxidase from Aspergillus niger, Nafion perfluorinated resin solution, L-ascorbic acid (AA), uric acid (UA), lactic acid (LA), and acetaminophen (AP) were obtained from Sigma–Aldrich. Potassium chloride, potassium phosphate dibasic, and potassium Phosphate monobasic were purchased from Fisherbrand. Silver/silver chloride (Ag/AgCl) paste was obtained from ERCON INC. E2414. Carbon-graphite mediator paste (C2070424P2) was purchased from Gwent Group, UK. Dragon SkinTM silicone rubber compound (DS) was used for the confinement of electrodes. All reagents were of analytical grade and were used without further purification.
Khosravi S., Soltanian S., Servati A., Khademhosseini A., Zhu Y, & Servati P. (2023). Screen-Printed Textile-Based Electrochemical Biosensor for Noninvasive Monitoring of Glucose in Sweat. Biosensors, 13(7), 684.
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9

Oxidative Stress Assessment Assay

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Chemicals and gases were obtained from Sigma-Aldrich unless otherwise indicated and were used without further purification: ascorbic acid (≥99.0 %), Chelex™ 100 sodium form, 0.1 M HCl solution, 0.1 M NaOH solution, dichlorofluorescin-diacetate (DCFH-DA), 1 M potassium phosphate buffer solution, horseradish peroxidase (HRP), methanol (HPLC grade), and o-phenylenediamine (≥99.5 %). H2O used for the DCFH, HRP and AA solution was obtained from a Milli-Q high-purity water unit (resistivity ≥18.2MΩcm−1, Merck Millipore, USA). For DTT analysis, 9,10-phenanthrenequinone (PQN) (≥99 %), 5,5’-dithiobis(2-nitrobenzoic acid) (DTNB) (99 %), DL-dithiothreitol (DTT) (≥98 %), potassium phosphate dibasic (≥98 %, Krebs buffer), potassium phosphate monobasic (≥98 %, Krebs buffer) and methanol (≥99.9 %) were all obtained from Fisher Chemical. Nitrogen (oxygen free) was obtained from BOC (Cambridge, UK).
Campbell S.J., Wolfer K., Utinger B., Westwood J., Zhang Z.H., Bukowiecki N., Steimer S.S., Vu T.V., Xu J., Straw N., Thomson S., Elzein A., Sun Y., Liu D., Li L., Fu P., Lewis A.C., Harrison R.M., Bloss W.J., Loh M., Miller M.R., Shi Z, & Kalberer M. (2021). Atmospheric conditions and composition that influence PM2.5 oxidative potential in Beijing, China. Atmospheric chemistry and physics, 21(7), 5549-5573.
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10

Recombinant GRFT Protein Preparation

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Recombinant GRFT drug substance was supplied by Kentucky Bioprocessing LLC (Owensboro, KY). The protein was supplied in a solution of PBS (12 mg/mL for 2-year stability study; 10 mg/mL for all other assessments). PBS 10× molecular biology grade (pH 7.4) was purchased from Mediatech, Inc. (Manassa, VA). Acetonitrile (ACN), trifluoroacetic acid (TFA), sodium acetate, formic acid, phosphate acid, potassium phosphate monobasic, potassium phosphate dibasic, Tris, hydrochloric acid (HCl), and sodium hydroxide (NaOH) were obtained from Fisher Scientific (Pittsburgh, PA). Hydrogen peroxide (H2O2, 30%), disodium ethylenediaminetetraacetic acid (EDTA), d-α tocopheryl polyethylene glycol 1000 succinate (vitamin E TPGS), ascorbic acid, methionine, glutathione, cysteine, aminopeptidase, lysozyme, and proteinase K were obtained from Sigma (St. Louis, MO). A MilliQ (Millipore; Milford, MA) water filtration system operating at 18.2 MΩ cm was used for water.
Kramzer L.F., Hamorsky K.T., Graebing P.W., Wang L., Fuqua J.L., Matoba N., Lasnik A.B., Moncla B.J., Zhang J., Palmer K.E, & Rohan L.C. (2021). Preformulation Characterization of Griffithsin, a Biopharmaceutical Candidate for HIV Prevention. AAPS PharmSciTech, 22(3), 83.
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