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Dri chem 7000v

Manufactured by Fujifilm
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Sourced in Japan

The DRI-CHEM 7000V is a compact, automated clinical chemistry analyzer designed for in-vitro diagnostic testing. It utilizes dry chemistry technology to rapidly analyze a variety of biochemical parameters from small sample volumes. The DRI-CHEM 7000V is capable of performing a range of common clinical chemistry tests.

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Lab products found in correlation

Labassay nefa, by Fujifilm (1 mentions) V cre p, by Fujifilm (1 mentions) V bun p, by Fujifilm (1 mentions) Acetate colorimetric assay kit, by Merck Group (1 mentions) Proline pro content assay kit, by Solarbio (1 mentions) Labassay nefa kit, by Fujifilm (1 mentions) Heparin tube, by Fujifilm (1 mentions) Poch 100iv, by Sysmex (1 mentions) Dri chem slide, by Fujifilm (1 mentions) Procyte, by IDEXX (1 mentions) Bio plex mouse cytokine 23 plex panel kit, by Bio-Rad (1 mentions) Ammonia assay kit, by Abcam (1 mentions) Colorimetric assay kit, by Merck Group (1 mentions) Lal chromogenic endpoint assay kit, by Hycult Biotech (1 mentions) Celltac alpha, by Nihon Kohden (1 mentions) Xt 2000i, by Sysmex (1 mentions) Oil red o, by Muto Pure Chemicals (1 mentions)

36 protocols using dri chem 7000v

1

APAP-Induced Acute Liver Injury in Mice

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Mice (female and 8–14 weeks of age for evaluation of the acute liver injury) were fasted for 24 h with free access to water but not bedding and feces. Fifteen mg per ml APAP (Sigma-Aldrich) was freshly prepared in 0.9% isotonic sodium chloride solution (Otsuka Pharmaceutical, Tokyo, Japan) at 37°C. WT and Ifng−/− mice and Rag1−/− mice were intraperitoneally injected with 100 and 150 mg/ kg body weight of APAP, respectively, and had free access to food, water, bedding, and feces after APAP injection. Blood was collected 18 h after APAP injection and plasma samples were prepared by centrifugation at 2500 rpm (500 xg) for 15 min at 4°C. Concentrations of ALT in the plasma were measured by using DRI-CHEM 7000V and GPT/ALT-PIII slides (Fujifilm).
To deplete CD25+ cells, Rag1−/− mice were intraperitoneally injected with 250 μg mAb against mouse CD25 (clone PC61) 6 h before and after the injection of APAP. Anti-mouse CD25 (clone 7D4) was used for staining CD25 after depletion of CD25+ cells by using PC61.
Nabekura T., Riggan L., Hildreth A.D., O’Sullivan T.E, & Shibuya A. (2019). Type 1 innate lymphoid cells protect mice from acute liver injury via interferon-γ secretion for upregulating Bcl-xL expression in hepatocytes. Immunity, 52(1), 96-108.e9.
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2

Biochemical Marker Quantification

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Serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), and total cholesterol (TC) were measured by a dry chemistry analyzer (FUJI DRI‐CHEM 7000 V, FUJIFILM, Tokyo, Japan) according to the manufacturer’s instructions.
Xia J., Guo W., Hu M., Jin X., Zhang S., Liu B., Qiu H., Wang K., Zhuge A., Li S., Ji Z., Li L, & Xu K. (2023). Resynchronized rhythmic oscillations of gut microbiota drive time-restricted feeding induced nonalcoholic steatohepatitis alleviation. Gut Microbes, 15(1), 2221450.
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3

Serum lipid levels at 36 weeks

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Blood serum levels of TG, TCHO, and HDL were measured at 36 weeks using a dry chemistry analyzer (DRI-CHEM 7000v, Fujifilm Corp., Tokyo, Japan).
Jabary M., Onoda A., Kitase Y., Ueda K., Mimatsu H., Go S., Miura R., Tsuji M., Takahashi Y., Hayakawa M, & Sato Y. (2023). Fetal growth restriction followed by early catch-up growth impairs pancreatic islet morphology in male rats. Scientific Reports, 13, 2732.
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4

Blood Lipid and Glucose Analysis

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The blood samples were centrifuged to separate the serum, and serum concentrations of total cholesterol and triglycerides were measured using a biochemical autoanalyzer (DRICHEM 7000V, FUJIFILM Medical Co., Ltd., Tokyo, Japan). Blood glucose levels were measured using a glucometer (ForaCare Japan Co., Ltd., Tokyo, Japan). Free fatty acid levels were measured by LabAssay NEFA (FUJIFILM Wako Pure Chemical Co., Ltd., Tokyo, Japan).
Ikeda K., Morizane S., Akagi T., Hiramatsu-Asano S., Tachibana K., Yahagi A., Iseki M., Kaneto H., Wada J., Ishihara K., Morita Y, & Mukai T. (2022). Obesity and Dyslipidemia Synergistically Exacerbate Psoriatic Skin Inflammation. International Journal of Molecular Sciences, 23(8), 4312.
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5

TP4O Inhibits Subcutaneous Tumor Growth

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To evaluate the effect of TP4O in vivo, a subcutaneous tumor was induced. HCT116 cells (2×106 cells per mouse) were injected subcutaneously into the right flanks of 14 mice. Tumor volume was calculated using the following formula: 0.5 × length × width2. When tumor volume had reached 80–100 mm3, the 12 qualifying mice were randomly divided into two groups (n=6/group) and subcutaneously injected with one of the following: 250 µl saline (control group) or 200 mg/kg TP4O dissolved in 250 µl saline (TP4O group). Each mouse was injected once every 3 days (total, five times). The tumor volume was recorded every 3 days. Body weight was recorded the first day of the injection, on day 7 and at the end of experimentation (day 14). On day 14, blood samples were collected under anesthesia, the mice were sacrificed and the tumors were excized. To evaluate TP4O toxicity, serum alanine aminotransferase (ALT) and serum creatinine levels were measured using an autoanalyzer (Dri-chem 7000 V; Fujifilm, Tokyo, Japan).
Nakayama K., Murata S., Ito H., Iwasaki K., Villareal M.O., Zheng Y.W., Matsui H., Isoda H, & Ohkohchi N. (2017). Terpinen-4-ol inhibits colorectal cancer growth via reactive oxygen species. Oncology Letters, 14(2), 2015-2024.
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6

Serum Creatinine and BUN Analysis in Rats

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Blood samples were drawn from every rat at weeks 2, 4, 6, and 8. After the rats were tightly caged, 2 ml of blood was collected from the tail vein using plain tubes. Blood samples were centrifuged at 3,500 rpm for 5 min, and clean non-hemolyzed serum was kept for analysis. The concentration of serum creatinine and blood urea nitrogen (BUN) were measured automatically by special kits (v-CRE-P and v-BUN-P, Fujifilm, Tokyo, Japan) and a Chemistry Analyzer (Dri-chem 7000V, Fujifilm, Tokyo, Japan).
Ma D., Mandour A.S., Elfadadny A., Hendawy H., Yoshida T., El-Husseiny H.M., Nishifuji K., Takahashi K., Zhou Z., Zhao Y, & Tanaka R. (2022). Changes in Cardiac Function During the Development of Uremic Cardiomyopathy and the Effect of Salvianolic Acid B Administration in a Rat Model. Frontiers in Veterinary Science, 9, 905759.
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7

Evaluating Intracerebroventricular HP-β-CD Effects on Liver Dysfunction in NPC1 Mice

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To examine the effect of intracerebroventricular HP-β-CD on the hepatic dysfunction occurring in Npc1−/− mice, serum transaminase levels and liver pathological changes were evaluated. Blood was collected from the inferior vena cava. The blood samples were centrifuged at 3000× g for 10 min at 4 °C and the serum was then collected. Serum ALT levels were measured using an automated analysis device (FUJI DRI-CHEM 7000 V; FUJIFILM Corporation, Tokyo, Japan). Liver samples were immediately weighed and some of the liver lobes were frozen in liquid nitrogen and stored at −80 °C for the measurement of GPNMB level. The other liver lobes were immediately fixed in 4% PFA, made into paraffin-embedded sections, and cut into 4-μm-thick slices for H&E staining. Histopathological images were obtained using Biorevo BZ-9000 microscope system.
Fukaura M., Ishitsuka Y., Shirakawa S., Ushihama N., Yamada Y., Kondo Y., Takeo T., Nakagata N., Motoyama K., Higashi T., Arima H., Kurauchi Y., Seki T., Katsuki H., Higaki K., Matsuo M, & Irie T. (2021). Intracerebroventricular Treatment with 2-Hydroxypropyl-β-Cyclodextrin Decreased Cerebellar and Hepatic Glycoprotein Nonmetastatic Melanoma Protein B (GPNMB) Expression in Niemann–Pick Disease Type C Model Mice. International Journal of Molecular Sciences, 22(1), 452.
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8

Biochemical Assessments after Pancreatectomy

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For biochemical assessments, including hepatic and renal function tests, serum was collected before the procedure and twice a week after partial pancreatectomy and STZ administration. Biochemical parameters were measured using an automatic analyser (DRI-CHEM 7000 V; Fujifilm, Tokyo, Japan). Spot urine samples were obtained twice a week and measured by a urine dipstick test.
Yuan W., Fukuda S., Inoue T., Okochi H., Sasaki E, & Shimoda M. (2019). Establishment of a diabetes mellitus type 1 model in the common marmoset. Scientific Reports, 9, 14546.
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9

Serum Metabolic Marker Analysis

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Collected blood was kept at room temperature for 90 min and was then centrifuged at 1,000 × g for 15 min at 20°C. The resulting supernatant was collected as serum and was stored at -80°C until needed for analysis. Metabolic markers in serum were analyzed using a Dri-Chem 7000V (Fuji Film, Japan).
Nakajima T., Vares G., Wang B, & Nenoi M. (2016). Chronic Intake of Japanese Sake Mediates Radiation-Induced Metabolic Alterations in Mouse Liver. PLoS ONE, 11(1), e0146730.
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10

Biochemical Analyses of Blood, Feces, and Brain

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Blood samples were centrifuged at 3000 g for 10 minutes at room temperature to separate serum. ALT, AST and ammonia concentrations were evaluated with a dry chemistry analyser (FUJI DRICHEM 7000 V, FUJIFILM, Tokyo, Japan). Fecal samples (100 mg) were suspended in 1 ml PBS, and supernatants were obtained by centrifuging at 13000 g for 10 minutes. The cerebrum of mice was fully ground in 500ul PBS, and the supernatants were spun at 13000 g for 10 minutes at room temperature to obtain the supernatants. The fecal and brain ammonia levels were also detected by the dry chemistry analyser. The acetate levels were determined with Acetate Colorimetric Assay Kit (Sigma-Aldrich, US). Proline levels were detected using the Proline (PRO) Content Assay Kit (Solarbio, China).
Wang Y., Li Y., Lv L., Zhu L., Hong L., Wang X., Zhang Y., Wang X, & Diao H. (2024). Faecal hsa-miR-7704 inhibits the growth and adhesion of Bifidobacterium longum by suppressing ProB and aggravates hepatic encephalopathy. NPJ Biofilms and Microbiomes, 10, 13.
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