Chloroform, ethyl acetate and methanol of reagent grade as well as HPLC-grade methanol and acetonitrile were purchased from Molar Chemicals Kft. (Halásztelek, Hungary). Dimethyl sulfoxide (DMSO), n-dodecane, sodium chloride (NaCl), hydrochloric acid (HCl), disodium hydrogen phosphate heptahydrate (Na2HPO4∙7H2O) and sodium dihydrogen phosphate monohydrate (NaH2PO4∙H2O) were obtained from Reanal-Ker (Budapest, Hungary), while phosphatidylcholine, cholesterol and the porcine polar brain lipid extract were purchased from Merck (Darmstadt, Germany). Acetic acid 100% for HPLC LiChropur™, pyruvate and PBS tablet (Phosphate Buffered Saline, pH 7.4) were acquired from Sigma-Aldrich (Steinheim, Germany). Roswell Park Memorial Institute 1640 medium (RPMI-1640) and Dulbecco’s Modified Eagle’s Medium (DMEM) were supplied by Lonza (Basel, Switzerland). Fetal bovine serum (FBS) was purchased from Biosera (Nuaille, France). Non-essential amino acids, penicillin/streptomycin (10,000 units penicillin and 10 mg streptomycin/mL) and trypsin were obtained from Gibco (Thermo Fisher Scientific, Waltham, MA, USA). High-purity water was gained by a Millipore Direct Q5 Water Purification System (Billerica, MA, USA).
Direct q 5 water purification system
Manufactured by Merck Group
Sourced in United States
The Direct-Q 5 Water Purification System is a laboratory equipment designed to produce high-quality ultrapure water. The system utilizes a multi-stage purification process to remove impurities from the input water, resulting in water that meets the stringent requirements for various laboratory applications.
Automatically generated - may contain errors
Lab products found in correlation
Phosphatidylcholine, by Merck Group (2 mentions)
Cholesterol, by Merck Group (2 mentions)
Irgacure 2959, by Merck Group (2 mentions)
Lsm 710, by Zeiss (2 mentions)
Porcine polar brain lipid extract, by Merck Group (1 mentions)
Penicillin, by Thermo Fisher Scientific (1 mentions)
Streptomycin, by Thermo Fisher Scientific (1 mentions)
Trypsin, by Thermo Fisher Scientific (1 mentions)
Non essential amino acids (neaa), by Thermo Fisher Scientific (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
Dulbecco s modified eagle s medium dmem, by Lonza (1 mentions)
Fetal bovine serum (fbs), by Biosera (1 mentions)
Ethyl acetate, by Molar Chemicals (1 mentions)
Methanol, by Molar Chemicals (1 mentions)
Acetonitrile, by Molar Chemicals (1 mentions)
Chloroform, by Molar Chemicals (1 mentions)
Dimethyl sulfoxide d6, by Merck Group (1 mentions)
Caffeine and rutin standards, by Merck Group (1 mentions)
Hydrochloric acid (hcl), by Reanal (1 mentions)
Sodium chloride (nacl), by Reanal (1 mentions)
7 protocols using direct q 5 water purification system
1
Lipid Extraction and Characterization Protocol
2
Fabrication of Porous POMaC Scaffolds
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To prepare poly(octamethylene maleate (anhydride) citrate) (POMaC) prepolymer, 1,8-octandiol, citric acid, and maleic anhydride were mixed at 5:1:4 molar ratio and melted at 160°C under nitrogen purge. The temperature was dropped to 140°C and the mixture was stirred for 2-3hr. The resultant pre-polymer solution was then dissolved in 1,6 dioxane and purified via drop-wise precipitation in deionized distilled water produced from a Direct-Q 5 Water Purification System (Millipore, Billerica, MA). Precipitated polymer was collected and lyophilized for 2 days. Prior to photo-crosslinking, POMaC prepolymer was mixed with 5% (w/w) UV initiator (Irgacure 2959, Sigma) by melting briefly at around 90°C. To make nano-porous scaffolds, POMaC polymer was also mixed with a porogen, poly(ethylene glycol) dimethyl ether (PEGDM, Mw~500, Sigma) at 60% (w/w) (Supplementary Figure 1
3
Fabrication of Porous POMaC Scaffolds
4
Comprehensive Analytical Protocol for Bioactive Compounds
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HPLC grade acetic and formic acid, myricetin-3-O-rhamnoside, quercetin-3-O-rhamnoside, hirsutenone, oregonin, caffeine and rutin standards, dimethyl sulfoxide-d6 (99.8 atom% D with 0.03 vol.% TMS) and methanol-d4 (99.8 atom% D), phosphatidylcholine, cholesterol and the porcine polar brain lipid extract were purchased from Merck (Darmstadt, Germany). Ethyl acetate, n-hexane and methanol of reagent grade, HPLC-MS grade acetonitrile, methanol, n-dodecane, dimethyl sulfoxide, NaCl, HCl, Na2HPO4·7H2O and NaH2PO4·H2O were obtained from Reanal-Ker (Budapest, Hungary). HPLC-grade water was prepared with a Millipore Direct Q5 water purification system (Bedford, MA, USA). All aqueous eluents for HPLC were filtered through MF-Millipore membrane filters (0.45 μm, mixed cellulose esters) (Billerica, MA, USA) and degassed in an ultrasonic bath before use.
5
Synthesis and Characterization of Metallosurfactant Complex
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The metallosurfactant complex was synthesized according to the protocol reported in literature [51 (link)]. Pure surfactant (diazobicyclooctane with hexadecyl tail) was prepared according to a previously published procedure [52 (link)]. 5,10,15,20-tetrakis (4-sulfonatophenyl) porphyrin dodecahydrate (Alfa Aesar, Ward Hill, MA, USA, 95%), rhodamine B (Acros Organics, Fair Lawn, NJ, USA, ≥98%), and cisplatin (Sigma-Aldrich, St. Louis, MO, USA) were used as received. All solutions were prepared in deionized water (18.2 MΩ) obtained using a Millipore Direct-Q 5 water purification system.
6
Amyloid-β Peptide Labeling and Characterization
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All commercially-available reagents and solvents were used as received without further purification. FAM-NHS was purchased from Lumiprobe Corp (Hunt Valley, Maryland, USA), sealed with parafilm and stored at −20°C. The purity and stability of the dye were monitored every 6 months using liquid chromatography mass spectrometry (LC-MS). Human amyloid-β peptide (1–42) was obtained from Anaspec (Fremont, CA, Cat. AS-20276). Ultrapure water (18.2 MΩ) was obtained from a Millipore Direct Q-5 water purification system. A Hitachi HPLC system (Lachrom Elite) equipped with a diode array detector was used for purification, and employed a Cytiva HiTrap Desalting column with a mobile phase of 25 mM sodium phosphate, 150 mM sodium chloride, pH7.4. Three fluorescence microscopes were used in our studies, including a manual Zeiss Axio Observer, an automated Zeiss Axio Observer, and a Zeiss LSM 710 confocal laser scanning microscope. MALDI-TOF analysis was performed on a Bruker Rapiflex with sinapinic acid matrix.
7
PVA-PEG-PVP Hydrophilic Polymer Formulation
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Poly (vinyl alcohol) (PVA, Mw = 85 -124 kDa, 87 -89% hydrolysed), poly (ethylene glycol) (PEG 4000, Mw = 400 and (PEG 10,000, Mw 10,000 Da), poly (vinyl pyrrolidone) K90 (PVP K90, Mw = 360 kDa) and hydroxypropyl methylcellulose (HPMC) with a viscosity of 40 -60 centipoise, 2% in H 2 O (20 °C) were purchased from Sigma-Aldrich (Dorset, UK).
Citric acid (CA) was supplied by BDH Laboratory Supplies (Poole, UK). PVP K29-32 (Mw = 58 kDa) was kindly donated by Ashland (Surrey, UK). Methotrexate disodium (MTX) (99.35% purity) was purchased from Haihang Industry Co., Ltd (Shandong, China).
Methotrexate polyglutamates (MTX-PG 1 -5 ) standards were purchased from Schircks Laboratories (Jona, Switzerland). Guthrie cards (Schleicher & Schuell 903) were purchased from Aston Ltd. (Oldham, England). Oasis solid-phase extraction (SPE) cartridges were obtained from Waters (Dublin, Ireland). Glycerine (99.5% purity) was purchased from VWR International (Lutterworth, England). HPLC grade water was produced using a Millipore Direct-Q™ 5 water purification system from Millipore (Watford, England). All other chemicals and materials were of analytical reagent grade supplied by Sigma-Aldrich (Dorset, UK) and Fisher Scientific (Loughborough, UK).
Citric acid (CA) was supplied by BDH Laboratory Supplies (Poole, UK). PVP K29-32 (Mw = 58 kDa) was kindly donated by Ashland (Surrey, UK). Methotrexate disodium (MTX) (99.35% purity) was purchased from Haihang Industry Co., Ltd (Shandong, China).
Methotrexate polyglutamates (MTX-PG 1 -5 ) standards were purchased from Schircks Laboratories (Jona, Switzerland). Guthrie cards (Schleicher & Schuell 903) were purchased from Aston Ltd. (Oldham, England). Oasis solid-phase extraction (SPE) cartridges were obtained from Waters (Dublin, Ireland). Glycerine (99.5% purity) was purchased from VWR International (Lutterworth, England). HPLC grade water was produced using a Millipore Direct-Q™ 5 water purification system from Millipore (Watford, England). All other chemicals and materials were of analytical reagent grade supplied by Sigma-Aldrich (Dorset, UK) and Fisher Scientific (Loughborough, UK).
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