BQ123 and/or BQ788 (both from Sigma, St. Louis, MO, USA) dissolved in sterile phosphate-buffered saline (PBS) were administered to corresponding SHAM and SCI mice (SHAM + BQ123, SHAM + BQ788, SHAM + BQ123 + BQ788, SCI + BQ123, SCI + BQ788 and SCI + BQ123 + BQ788) via intraperitoneal injection (10 mg/kg, respectively) (27 (link)–29 (link)) at 1 day before SCI and then further treated once a day for 6 weeks for behavioral testing or for the indicated time-points (2, 4, 6 and 24 h and 4 days) for other experiments post-injury. PBS for vehicle control (VEH) was administered in corresponding SHAM and SCI mice (SHAM + VEH and SCI + VEH). Significant side effects resulting from BQ123 and/or BQ788 treatment, such as changes in body weight or an increase in mortality, were not observed during our experiments.
Bq788
Manufactured by Merck Group
Sourced in United States, Germany
BQ788 is a precision laboratory equipment designed for specialized scientific applications. It functions as a high-performance analytical device, providing accurate and reliable measurements for researchers and scientists. The core function of BQ788 is to enable precise data collection and analysis within controlled laboratory environments.
Automatically generated - may contain errors
Lab products found in correlation
Bq123, by Merck Group (18 mentions)
Phenylephrine, by Merck Group (2 mentions)
L name, by Merck Group (2 mentions)
Fenofibrate, by Merck Group (1 mentions)
Gw6471, by Santa Cruz Biotechnology (1 mentions)
Pd98059, by Cell Signaling Technology (1 mentions)
Sb203580, by Cell Signaling Technology (1 mentions)
Gm6001, by US Biological (1 mentions)
Hoe 140, by Merck Group (1 mentions)
Fucoidan, by Merck Group (1 mentions)
L nmma, by Merck Group (1 mentions)
Methysergide, by Merck Group (1 mentions)
Anti tnf α, by R&D Systems (1 mentions)
Promethazine, by Merck Group (1 mentions)
Anti il 1β, by R&D Systems (1 mentions)
Anti il 6, by R&D Systems (1 mentions)
Anti tra 1 60, by Merck Group (1 mentions)
Alexa fluor 488 chicken anti rabbit igg, by Thermo Fisher Scientific (1 mentions)
Alexa fluor 594 goat anti mouse igg1, by Thermo Fisher Scientific (1 mentions)
Goat anti mouse igg2b alexa fluor 594, by Thermo Fisher Scientific (1 mentions)
25 protocols using bq788
1
BQ123 and BQ788 in Spinal Cord Injury
2
Human Cardiac Myocyte Experiments
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HCMs were purchased from ScienCell Research Laboratories (Carlsbad, CA, USA) and grown in cardiac myocyte medium supplemented with 5% fetal bovine serum (FBS), 1% cardiac myocyte growth supplement, and 1% penicillin/streptomycin solution. Cells were cultured on a poly-L-lysine-coated dish in a humidified incubator with 5% CO2 at 37°C. Cells between passages 3–5 were used in all experiments. The Safety Committee of Biological Experiments at Kaohsiung Medical University approved the use of HCMs for in vitro experimental use. The following drugs were used: fenofibrate (a PPARα agonist, Sigma), GW6471 (GW, PPARα antagonist, Santa Cruz), BQ-123 [BQ123, endothelin A receptor (ETA) antagonist, Sigma], BQ788 [BQ788, endothelin B receptor (ETB) antagonist, Sigma], PD98059 (ERK inhibitor, Cell Signaling), and SB203580 (p38 inhibitor, Cell Signaling). None of these drugs significantly influence cell viability (>90%).
3
Endothelin Receptor Antagonists in GDM
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The effects of ETA receptor antagonist BQ123 and ETB receptor antagonist BQ788 were assessed on omental arteries collected from pregnancies complicated by GDM (treated with insulin, n = 5) and healthy gestation-matched controls (n = 3–8). To investigate differences in the function of ETA and ETB receptors in GDM, following confirmation of artery integrity [20 (link),21 (link)], arteries were incubated in BQ123 (1 µM; Sigma-Aldrich, St Louis, MO, USA), BQ788 (5 µM; Sigma-Aldrich, St Louis, MO, USA), a combination of BQ123 (1 µM) and BQ788 (5 µM), or vehicle control (ethanol) prior to constriction with recombinant ET-1 (10−11 M to 10−7 M). Following treatment, the antagonist or vehicle control treatment remained in the bath with ET-1 (concentrations as detailed above). Changes in measured constriction were normalized to the pre-determined maximum constriction induced by KPSS.
4
Cytokine Inhibition in Inflammation
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Anti-IL-1β, anti-IL-6, anti-TNFα and anti-CINC-1 antibodies were supplied by R&D Systems Inc. (USA). Indomethacin, AACOCF3 and PACOCF3 were purchased from Biomol Research Laboratories (USA). GM6001 was supplied by USBiological (USA); whereas L-NMMA, HOE 140, Lys-(Des-Arg9,Leu8)-bradykinin, promethazine, methysergide, BQ-123, BQ-788 and fucoidan were purchased from Sigma-Aldrich Co. (USA). Celecoxib was supplied by Searle and Co (Puerto Rico). Zileuton was purchased from Abbott Laboratories (Zyflo®, USA). Carrageenin was purchased from Marine Colloids.
5
Circadian Regulation of Metabolic Processes
6
Immunofluorescence Staining of Pluripotency and Cardiac Markers
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Immunofluorescence staining for pluripotency of iPSCs was performed by using the following primary antibodies: anti‐NANOG (Abcam), anti‐OCT3/4 (Santa Cruz), anti–SSEA 3 (Millipore), anti–SSEA 4 (Millipore), anti–Tra‐1‐60 (Millipore), and anti–Tra‐1‐81 (Millipore). In immunofluorescence staining for cardiac markers, monoclonal anti–α‐actinin (Sigma), monoclonal anti‐cTnT (Thermo Scientific), polyclonal anti‐cTnT (Santa Cruz), monoclonal anti–myosin light chain (MLC)2a (Synaptic Systems), polyclonal anti‐MLC2v (ProteinTech Group), anti‐ANP (Santa Cruz), anti–cMyBP‐C (Santa Cruz), polyclonal anti–cMyBP‐C motif (supplied by C. Witt University of Heidelberg, Heidelberg, Germany), and anti–nuclear factor of activated T cells (NFAT)c4 (Santa Cruz) were used. The isotype‐specific secondary antibodies, Alexa Fluor 488 chicken anti‐rabbit IgG, Alexa Fluor 594 goat anti‐mouse IgG1, Alexa Fluor 488 goat anti‐rat IgM, Alexa Fluor 594 goat anti‐mouse IgM, Alexa Fluor 488 goat anti‐mouse IgG, Alexa Fluor 594 goat anti‐mouse IgG2b, Alexa Fluor 594 chicken anti‐goat IgG, and Alexa Fluor 555 goat anti‐rabbit IgG, were all obtained from Invitrogen. The tested drugs included endothelin‐1 (1.0, 10, 100, or 1000 nmol/L), angiotensin II (100 nmol/L), insulin‐like growth factor 1 (100 nmol/L), phenylephrine (0.05 mmol/L), BQ‐123 (250 nmol/L), and BQ‐788 (100 nmol/L) (all from Sigma).
7
Endothelin-1 Regulation in HTR8/SVneo Trophoblasts
8
Endothelin Receptor Antagonists in Mice
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Adult male C57BL6/J mice (Jackson Labs, age 6–8 weeks) were used for this study. All drugs and vehicles were administered via osmotic minipumps (Model 1002, Alzet, Cupertino, CA, USA) implanted subcutaneously in the dorsal region under isoflurane anesthesia (1–2% isoflurane in oxygen, Baxter, Deerfield, IL, USA) and incisions closed with 2–3 sutures. Pumps were filled with 100 µl of: (a) BQ-123 (0.407 mg/mL dissolved in saline; American Peptide, Sunnyvale, CA, USA), a selective ETA antagonist; (b) BQ-788 (0.509 mg/mL dissolved in propylene glycol; American Peptide, Sunnyvale, CA, USA), a selective ETB antagonist; (c) saline (vehicle for BQ-123) or (d) propylene glycol (vehicle for BQ-788; Sigma-Aldrich, St. Louis, MO, USA). The osmotic minipumps delivered a constant flow-rate of 0.25 μl/h, resulting in a dose of 200 nmol/kg/day of BQ-123 or BQ-788 in each animal (~25 g body weight).
9
Neutrophil Response to ET-1 Signaling
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Three hours after treatment with TNFα with or without ET receptor antagonists, mice were anesthetized; blood was collected on EDTA by intracardiac puncture and immediately processed for flow cytometry staining for viability and CD11a, CD11b and CD62L surface expression on Ly5G+ neutrophils (see Online Supplementary Methods).
Isolated human neutrophils were fluorescently labeled with fluo-4-acetoxymethyl ester (Fluo-4-AM; Invitrogen). Neutrophils were incubated with BQ123 or BQ788 (both from Sigma®, Saint-Quentin Fallavier, France) at 10 μmol/L for 15 min and analyzed immediately by flow cytometry (FACSCanto II, BD Biosciences, Sparks, MD, USA). After 2 min of signal acquisition, the analysis was interrupted for less than 10 s to add 100 nM of ET-1 (Sigma®) to the tube before the analysis was resumed.
Isolated human neutrophils were fluorescently labeled with fluo-4-acetoxymethyl ester (Fluo-4-AM; Invitrogen). Neutrophils were incubated with BQ123 or BQ788 (both from Sigma®, Saint-Quentin Fallavier, France) at 10 μmol/L for 15 min and analyzed immediately by flow cytometry (FACSCanto II, BD Biosciences, Sparks, MD, USA). After 2 min of signal acquisition, the analysis was interrupted for less than 10 s to add 100 nM of ET-1 (Sigma®) to the tube before the analysis was resumed.
10
Intradermal Microdialysis for Endothelin-1 and Norepinephrine
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