Molf eij

Manufactured by Jackson ImmunoResearch

Sourced in Montenegro

The MOLF/EiJ is a mouse strain developed for research purposes. It is an inbred strain derived from wild mice originating from Japan. This strain is commonly used in genetic and immunological studies.

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7 protocols using molf eij

Mouse Strain Acclimation and Housing

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Male (n = 5/strain) and female (n = 5/strain) mice were purchased at 8 weeks of age and allowed 14 days to acclimate prior to starting experiments. Most strains were purchased from the Jackson Laboratory (C57BL/6 J (B6), catalog number 000664; BALB/cJ, catalog number 000651; DBA/1 J, catalog number 000670; FVB/NJ, catalog number 001800; 129X1/SvJ, catalog number 000691; MOLF/EiJ, catalog number 000550). CD1 (ICR) mice were purchased from Charles River laboratories (catalog number 022). Mice were maintained within the Case Western Reserve University animal vivarium under specific-pathogen-free conditions and provided standard rodent chow diet (4.5% fat by weight, 14% kcal, Lab Diet P3000).

Swindell W.R., Michaels K.A., Sutter A.J., Diaconu D., Fritz Y., Xing X., Sarkar M.K., Liang Y., Tsoi A., Gudjonsson J.E, & Ward N.L. (2017). Imiquimod has strain-dependent effects in mice and does not uniquely model human psoriasis. Genome Medicine, 9, 24.

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Mouse Strain Characterization for Immunology

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A total of 23 mouse strains (129X1/SvJ, A/J, AKR/J, B10.S-H2s/SgMcdJ (B10.S), BALB/cJ, BPL/1J, BPN/3J, C3H/HeJ, C57BL/6J, C57BL/10J, CBA/J, CZECHII/EiJ, DBA/1J, DBA/2J, FVB/NJ, JF1/MsJ, MOLF/EiJ, MRL/MpJ, NOD/ShiLtJ, NU/J, PWD/PhJ, PWK/PhJ, SJL/J and SWR/J were purchased from the Jackson Laboratory (Bar Harbor, ME). All mice, including B10.S-Histh^SJL and B10.S-Histh^SJL ISRC lines, were generated and maintained under specific pathogen-free conditions in the vivarium of the Given Medical Building at the University of Vermont according to National Institutes of Health guidelines. All animal studies were approved by the Institutional Animal Care and Use Committee of the University of Vermont.

Tyler A.L., Raza A., Krementsov D.N., Case L.K., Huang R., Ma R.Z., Blankenhorn E.P., Teuscher C, & Mahoney J.M. (2019). Network-Based Functional Prediction Augments Genetic Association To Predict Candidate Genes for Histamine Hypersensitivity in Mice. G3: Genes|Genomes|Genetics, 9(12), 4223-4233.

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Transgenic Mouse Models of Breast Cancer

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FVB/N-Tg(MMTV-PyVT)634Mul/J and FVB/N-Tg(MMTVneu)202Mul/J male mice were obtained from The Jackson Laboratory. FVB/N-Tg(C3(1)-TAg) and FVB/N-Tg(MMTV-Myc) were a generous gift from Dr. Jeffrey Green (NCI, Bethesda, MD).
Male PyMT mice were crossed with female wild type FVB/NJ, MOLF/EiJ, CAST/EiJ, C57BL/6J, and C57BL/10J mice also obtained from The Jackson Laboratory. Male Her2, Myc, and C3(1)-TAg mice were crossed with female wild type FVB/NJ mice. All female F1 progeny were genotyped by the Laboratory of Cancer Biology and Genetics genotype core for the PyMT or Her2 gene and grown until humane endpoint. Primary tumor, metastatic nodules, and normal (tail) tissue were isolated immediately following euthanasia and snap-frozen in liquid nitrogen. Tissue samples were then stored at −80°C.

Ross C., Szczepanek K., lee M., Yang H., Peer C.J., Kindrick J., Shankarappa P., Lin Z.W., Sanford J., Figg W.D, & Hunter K. (2020). Metastasis-specific gene expression in autochthonous and allograft mouse mammary tumor models: stratification and identification of targetable signatures.. Molecular cancer research : MCR, 18(9), 1278-1289.

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Sourcing and Housing Inbred Mouse Strains

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The following inbred mouse strains were obtained from Jackson Laboratories (Bar Harbor, ME): C57BL/6J, CAST/EiJ, MOLF/EiJ, C58/J, NZW/LacJ, CASA/RkJ, and F1 and back-cross progeny. BALB/c mice were obtained from Taconic Biotechnology, Germantown, NY). Mice were maintained in small, ventilated microisolator cages.

Earl P.L., Americo J.L, & Moss B. (2015). Genetic studies of the susceptibility of classical and wild-derived inbred mouse strains to monkeypox virus. Virology, 481, 161-165.

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Genetic Mapping of STING Pathway

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C57BL6/J and MOLF/EiJ mice were from Jackson Laboratories (Bar Harbor, ME), STING-deficient mice - from Glen Barber (U. Miami). B6.MOLF-Tmem173^molf (STING^MOLF/MOLF) congenic mice were established via genotyping of the backcross mice at Tmem173 locus. A panel of F2 intercross mice was generated by, first, producing F1 (C57BL6/J × MOLF) mice followed by brother-sister intercrossing. Mice were phenotyped at the age of 6-8 weeks. For phenotyping, peritoneal macrophages were plated at a density of 10⁵ cells/well of the 96-well plate and transfected (Lipofectamine 2000) next day with c-di-AMP or c-di-GMP (2ug/mL) for 16 hrs. Genome wide genotyping of the F2 panel of mice was performed with 3 polymorphic microsatellite markers per chromosome using MIT primer sequences obtained from The Jackson Laboratory Mouse Genome Informatics Web site: http://www.informatics.jax.org/marker. PCR fragments were amplified using Jumpstart REDtaq (Sigma) and resolved on 3% gels. J/qtl v.1.3.x and R.2.0 software was used for linkage analysis. For generation of the B6.MOLF-Tmem173^molf (STING^MOLF/MOLF) congenic mice, F1 (B6 × MOLF) hybrids were backcrossed for 9 generation to C57BL6/J. Phenotyping assays with congenic mice were done using 8-12 week-old females.

Surpris G., Chan J., Thompson M., Ilyukha V., Liu B.C., Atianand M., Sharma S., Volkova T., Smirnova I., Fitzgerald K.A, & Poltorak A. (2015). Novel Tmem173 allele reveals importance of STING N-terminus in trafficking and type I IFN production. Journal of immunology (Baltimore, Md. : 1950), 196(2), 547-552.

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Mouse Strain Characterization of AAV

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All procedures were performed as approved by the Broad Institute IACUC (0213-06-18) or Massachusetts General Hospital IACUC (2014N000005; AAVR experiments). AKR/J (000648), BALB/cJ (000651), CBA/J (000656), CAST/EiJ (000928), C57Bl/6J (000664), C57BL/J (000668), DBA/2J (000671), FVB/NJ (001800), LP/J (000676), MOLF/EiJ (000550), NOD/ShiLtJ (001976), NZB/B1NJ (000684), and PWK/PhJ (003715) were obtained from the Jackson Laboratory (JAX). AAVR KO mice were a generous gift from Dr. J.E. Carette (Stanford) to Dr. Balazs and have been previously described [39 (link)]. Recombinant AAV vectors were administered intravenously via the retro-orbital sinus in young adult male or female mice. Mice were randomly assigned to groups based on predetermined sample sizes. No mice were excluded from the analyses. Experimenters were not blinded to sample groups.

Huang Q., Chan K.Y., Tobey I.G., Chan Y.A., Poterba T., Boutros C.L., Balazs A.B., Daneman R., Bloom J.M., Seed C, & Deverman B.E. (2019). Delivering genes across the blood-brain barrier: LY6A, a novel cellular receptor for AAV-PHP.B capsids. PLoS ONE, 14(11), e0225206.

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Murine Models for Breast Cancer

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FVB/N-Tg(MMTV-PyVT)634Mul/J (PyMT) and FVB/N-Tg(MMTVneu)202Mul/J (Her2) male mice were obtained from Jackson Labs. Male PyMT mice were crossed with female wild type FVB/NJ, MOLF/EiJ, CAST/EiJ, C57BL/6J, and C57BL10/J mice also obtained from Jackson Labs. Male Her2 mice were crossed with female wild type FVB/NJ mice. All female F1 progeny were genotyped by the Laboratory of Cancer Biology and Genetics genotype core for the PyMT or Her2 gene and grown until humane endpoint. Mice were euthanized using intraperitoneal Avertin to anesthetize followed by cervical dislocation. All primary tumors generated by one animal were isolated weighed, randomly sampled, and combined into a single cryovial. Metastatic nodules, and normal (tail) tissue were also isolated immediately following euthanasia and snap frozen in liquid nitrogen. Tissue samples were then stored at -80°C.

Ross C., Szczepanek K., Lee M., Yang H., Qiu T., Sanford J.D, & Hunter K. (2020). The genomic landscape of metastasis in treatment-naïve breast cancer models. PLoS Genetics, 16(5), e1008743.

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