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1 5 diaminonaphtalene 1 5 dan 97.0 maldi matrix

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1,5-diaminonaphthalene (1,5-DAN, ≥97.0%) is a MALDI matrix. MALDI matrices facilitate the ionization of analytes during MALDI mass spectrometry analysis.

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2 protocols using 1 5 diaminonaphtalene 1 5 dan 97.0 maldi matrix

1

MALDI-MRMS Imaging Mass Spectrometry

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All reagents were of HPLC grade. Milli-Q water (ddH2O; Millipore) was prepared in-house. Conductive indium tin oxide (ITO)-coated glass slides were purchased from Bruker Daltonics (Bremen, Germany). Adhesive slides SuperFrost PlusTM were obtained from Thermo Fisher Scientific (Waltham, Massachusetts, USA) for histological analysis. Trifluoroacetic acid (TFA) and 1,5-diaminonaphtalene (1,5-DAN, ≥97.0%) MALDI matrix were purchased from Sigma-Aldrich (St. Louis, MO). 4-phenyl-α-cyanocinnamic acid amide (PhCCAA) matrix was obtained from SiChem (Bremen, Germany). Acetonitrile (ACN) was obtained from VWR (Darmstadt, Germany). For external calibration of the Bruker solarix magnetic resonance mass spectrometer (MRMS), a mixture of poly-DL-alanine (10 mg/mL), L- alanine ≥ 99.5% (5 mg/mL), and taurine ≥ 99% (5 mg/mL; all from Sigma-Aldrich) was used in water.
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2

MALDI-MRMS Metabolite Profiling

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All reagents were of HPLC grade. Milli-Q water (ddH2O; Millipore) was prepared in-house.
Conductive indium tin oxide (ITO) coated glass slides were purchased from Bruker Daltonics (Bremen, Germany). Adhesive slides SuperFrost Plus TM were obtained from Thermo Fisher Scientific (Waltham, Massachusetts, USA) for histological analysis. Trifluoroacetic acid (TFA) and 1,5-diaminonaphtalene (1,5-DAN, ≥ 97.0%) MALDI matrix were purchased from Sigma-Aldrich (St. Louis, MO). Acetonitrile (ACN) was obtained from VWR (Darmstadt, Germany). For external calibration of the Bruker solarix magnetic resonance mass spectrometer (MRMS), a mixture of poly-DL-alanine (10 mg/mL), L-alanine ≥ 99.5% (5 mg/mL) and taurine ≥ 99% (5 mg/mL; all from Sigma Aldrich) in water was used.
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