Anti epac2 antibody 5b1

Manufactured by Cell Signaling Technology

The Anti-Epac2 antibody (5B1) is a monoclonal antibody that specifically recognizes the Epac2 protein. Epac2 is a guanine nucleotide exchange factor that plays a role in cAMP-mediated signaling pathways. This antibody can be used for the detection of Epac2 in various experimental applications.

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Lab products found in correlation

Rolipram, by Merck Group (2 mentions) Microcon filter, by Merck Group (2 mentions) Anti cd31 antibody dia 310, by Dianova (2 mentions)

2 protocols using anti epac2 antibody 5b1

Radiolabeled Human Serum Albumin Protocol

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The anti‐Epac1 antibody was kindly provided by J. Bos, University of Utrecht, the Netherlands. The anti‐Epac2 antibody (5B1) was from Cell Signaling technology (www.cellsignal.com). The anti‐CD31 antibody (DIA‐310) was from Dianova, Hamburg, Germany. ANP and rolipram were from Sigma‐Aldrich (St. Louis, MO, USA). Gadomer‐17 was from in vivo Contrast GmbH, Schering Pharma (Berlin, Germany). Human serum albumin (HSA) was labelled with ¹³¹I or ¹²⁵I (from Perkin Elmer, Waltham, MA, USA) by the iodogen method, as previously described (Curry et al. 2010). Any free iodide was removed by spinning twice on a 3‐kDa cut‐off column (Microcon filter; Millipore, Bedford, MA, USA) prior to experiments. The labelled HSA eluted like native HSA upon anionic exchange and high‐resolution size exclusion chromatography with online gamma radiation detection in series with UV detector. Low molecular weight radioactivity accounted for <0.3% of the total activity.

Kopperud R.K., Rygh C.B., Karlsen T.V., Krakstad C., Kleppe R., Hoivik E.A., Bakke M., Tenstad O., Selheim F., Lidén Å., Madsen L., Pavlin T., Taxt T., Kristiansen K., Curry F.‐., Reed R.K, & Døskeland S.O. (2016). Increased microvascular permeability in mice lacking Epac1 (Rapgef3). Acta Physiologica (Oxford, England), 219(2), 441-452.

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Radiolabeling of Human Serum Albumin

Check if the same lab product or an alternative is used in the 5 most similar protocols

The anti-Epac1antibody was kindly provided by J. Bos, Univ. of Utrecht, The Netherlands. The anti-Epac2 antibody (5B1) was from Cell Signaling technology (www.cellsignal.com). The anti-CD31 antibody (DIA-310) was from Dianova, Hamburg, Germany). ANP and rolipram were from Sigma-Aldrich (St. Louis, MO, USA). Gadomer 17 was from in vivo Contrast GmbH, Schering Pharma (Berlin, Germany). Human serum albumin (HSA) was labeled with ¹³¹I or ¹²⁵I (from Perkin Elmer, Massachusetts, USA) by the Iodogen method, as previously described (Curry et al., 2010 (link)). Any free iodide was removed by spinning twice on a 3 kDa cut-off column (Microcon filter; Millipore, Bedford, MA, USA) prior to experiments. The labeled HSA eluted like native HSA upon anionic exchange and high-resolution size exclusion chromatography with online gamma radiation detection in series with UV detector. Low molecular weight radioactivity accounted for less than 0.3% of the total activity.

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