Hydrochloroauric acid trihydrate (HAuCl4·3H2O) and caffeic acid were purchased from Sigma-Aldrich (St. Louis, MO, USA). All other reagents were of analytical grade. Deionized water was used to prepare all of the solutions. The UV-visible spectra were acquired with a Shimadzu UV-2600 spectrophotometer (Shimadzu Corporation, Kyoto, Japan). A JEM-3010 electron microscope was used to obtain the HR-TEM images at 300 kV (JEOL, Tokyo, Japan). The mean particle size by dynamic light scattering and zeta potential were measured on a Brookhaven 90Plus at 20 °C (Brookhaven Instruments Co., Holtsville, NY, USA).
Jem 3010 electron microscope
Manufactured by JEOL
Sourced in Japan
The JEM-3010 is a transmission electron microscope (TEM) designed and manufactured by JEOL. It is a high-performance instrument capable of providing detailed images of small-scale structures and materials. The JEM-3010 utilizes an electron beam to illuminate and magnify samples, allowing users to observe their internal structure and composition at the nanoscale level.
Automatically generated - may contain errors
Lab products found in correlation
Hydrochloroauric acid trihydrate, by Merck Group (1 mentions)
Caffeic acid, by Merck Group (1 mentions)
Uv 2600 spectrophotometer, by Shimadzu (1 mentions)
Brookhaven 90plus, by Brookhaven Instruments (1 mentions)
Nanosight ns300 analyzer, by Malvern Panalytical (1 mentions)
Rf 6000, by Shimadzu (1 mentions)
Litesizer 500, by Anton Paar (1 mentions)
K alpha instrument, by Thermo Fisher Scientific (1 mentions)
V 570 uv vis spectrophotometer, by Jasco (1 mentions)
D8 advance x ray powder diffractometer, by Bruker (1 mentions)
E 112, by Agilent Technologies (1 mentions)
Xps k alpha surface analysis, by Thermo Fisher Scientific (1 mentions)
F 7000 fluorescence spectrophotometer, by Hitachi (1 mentions)
Toc l, by Shimadzu (1 mentions)
Jem 2010 electron microscope, by JEOL (1 mentions)
Zetaplus apparatus, by Brookhaven Instruments (1 mentions)
Uv 1800 uv vis spectrophotometer, by Shimadzu (1 mentions)
Zeta size analyzer, by Malvern Panalytical (1 mentions)
Libra s60, by Harvard Bioscience (1 mentions)
Spectrum 400 spectrophotometer, by PerkinElmer (1 mentions)
7 protocols using jem 3010 electron microscope
1
Synthesis of Gold Nanoparticles
2
Exosome Isolation and Characterization
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Exosomes were isolated according to the previous report.30 In brief, the supernatants of cultured cells and peripheral blood were sequentially centrifuged at 2000 g and 4°C for 20 minutes to remove dead cells, and were further centrifuged at 10 000 g and 4°C for 30 minutes to remove cell debris. The supernatants were then transferred to another clean ultra tube and ultracentrifuged at 100 000 g and 4°C for 70 minutes. After discarding the supernatants, the deposited exosomes were resuspended in PBS, and then ultracentrifuged at 100 000 g and 4°C for another 70 minutes. The purified exosomes were resuspended with PBS for further study or stored at −80°C. Immunoblots were conducted to determine the specific expression markers of exosomes, including CD63, CD9, and TSG101. The size and amount of exosomes were identified by nanoparticle tracking analysis on a Nanosight NS300 analyzer (Malvern Instruments Ltd, Malvern, UK), and the morphology of exosomes was photographed by JEM‐3010 electron microscope (JEOL).
3
Characterization of CuInS/ZnS QDs
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The synthesized TPPS4, CuInS/ZnS QDs, CuInS/ZnS–TPPS4 conjugate were characterized using Fourier Transform infrared spectroscopy (FTIR) (Spectrum), two UATR spectrometer (Perkin Elmer, UK), photoluminescence (PL) (RF-6000, Shimadzu, Japan), ultraviolet–visible spectrophotometry (UV–vis) (Perkin Elmer UV–Vis Lambda 25 spectrometer, UK), Nuclear Magnetic Radiation (NMR, 500 MHz Bruker spectrometer); Zeta potential analyses were performed using Anton Paar Litesizer 500 (Austria). TEM was done by using JEOL JEM-3010 electron microscope operating at 200 kV.
4
Comprehensive Characterization of Synthesized Products
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Phase purity and crystallite size of synthesized products were analyzed by Bruker D8 Advance powder X-Ray Diffractometer (Bruker AXS GmbH, Karlsruhe, Germany) with CuKa source. The morphology and particle size of synthesized products were examined using Transmission Electron Spectroscopy (TEM). TEM has been recorded employing JEOL JEM 3010 electron microscope (JEOL Ltd., Tokyo, Japan). Photoluminescence spectrum (PL) was recorded using Hitachi F-7000 Fluorescence spectrophotometer with 150 W Xe lamp as excitation source. The slit width at excitation and emission were 5 nm. UV-Visible spectra were recorded at room temperature using Jasco V 570 UV-Vis spectrophotometer. X-Ray photoelectron spectroscopic analysis was done using K-Alpha instrument (XPS K-Alpha surface analysis, Thermo fisher scientific, UK). X-band EPR was recorded using Varian E 112 at room temperature. Total Organic Content (TOC) was measured using Shimadzu TOC-L.
5
Visualizing Composite Nanofiber Structure
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Transmission electron microscopy (TEM) images of the HA and the composite nanofiber COHA were done, using a JEOL JEM-3010 electron microscope (Tokyo, Japan) operating at 200 kV. The clay sample was dispersed well in ethanol and a drop was casted on the TEM grid followed by drying. For the TEM image of the composite nanofiber, the COHA solution was spin coated on a copper grid for 5 s and then dried and viewed under the microscope.
6
Characterization of Nanoparticle Dispersions
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UV-Vis spectra were recorded on a Shimadzu UV-1800 UV-Vis spectrophotometer. Transmission electron microscopy (TEM) observations were carried out with a JEOL JEM-2010 electron microscope with an acceleration voltage of 100 kV. High-resolution TEM (HRTEM) images were obtained by a JEOL JEM-3010 electron microscope operated with an acceleration voltage of 300 kV. The dispersions were dropped onto carbon-coated copper grids and then dried in air before the TEM observations. Temporal evolutions of the UV-Vis spectra were acquired by an Ocean Optics HR4000CG-UV-NIR high-resolution spectrophotometer. Zeta-potential was measured on a Brookhaven ZetaPlus apparatus. The pH of samples was adjusted from 2.5 to 8.0 by using Britton–Robinson buffer (mixture of 2 mM acetic acid, phosphate acid and boric acid with different amount of NaOH). Power XRD patterns were recorded on a Rigaku D-Max 2550 diffractometer equipped with a graphite monochromator using Cu Kα radiation (λ = 1.54 Å) at a scanning speed of 5° min−1. The samples for XRD measurements were dried frozenly in vacuo. Raman spectra were obtained by using a Horiba LabRAM UR evolution instrument with 785 nm laser (50 mW) and a holder for cuvette-based liquid measurements. The accumulation time was 60 s for all the data presented.
7
Characterization of Silver Nanoparticles
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A UV spectrophotometer (Biochrom Libra S60, Cambridge, UK) was used to measure the absorption behavior of the Ag-NPs in the range of 200–900 nm. For analyzing the UV, the sample was diluted with water in the ratio 1:2. FT-IR was done using a PerkinElmer Spectrum 400 spectrophotometer (Waltham, MA, USA) in the range 400–4000 cm−1 with a resolution of 2 cm−1. For measuring FT-IR, the sodium alginate powder and the alginate-capped Ag-NPs solution were used. For zeta potential measurements (Malvern Zeta size analyzer, UK), the sample was diluted with water in the ratio 1:2 to avoid errors. X-ray diffraction analysis was performed using a diffractometer (PANalytical model X’PERT-PRO, Malvern, UK) with Kα radiation of 1.5418 Å. The Ag-NPs solution was dried in the oven and crushed using a mortar to prepare fine powdered nanoparticles for XRD measurements. TEM and HRTEM measurements were performed using a JEOL JEM-3010 electron microscope (Japan) operating at 200 kV. For TEM analysis, the sample was prepared by casting a single micro drop of the Ag-NPs solution on a TEM grid and dried.
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