Eight days after the start of HF, rats were pseudo -randomized into four groups (Young Control, n=6; Young DIZE, n=6; Old Control, n=8; Old DIZE, n=9) based on body weight to ensure that rats of various weights were represented equally in each group , and given either 15 mg/kg/day DIZE (LKT Laboratories Inc.; St. Paul, MN) or vehicle (water) s.c. Body weight and food intake were measured daily during the first week to document the hyperphagic response to the introduction of the HF diet and then subsequently measured twice weekly. Food and water were provided ad libitum in a food hopper that rested inside the cage above the animal. Daily food intake was measured by placing all food pellets remaining in the hopper on the scale. Body composition was measured at weeks 1 and 3 after treatment began via time-domain nuclear magnetic resonance (TD-NMR) in restrained but fully conscious rats (TD-NMR Minispec, Bruker Optics, The Woodlands, TX, USA). Treatment lasted for three weeks, and animals were sacrificed 24 hrs after final DIZE injection.
Td nmr minispec
Manufactured by Bruker
Sourced in United States
The TD-NMR Minispec is a compact time-domain nuclear magnetic resonance (TD-NMR) spectrometer designed for various analytical applications. It provides rapid, non-destructive measurements of physical and chemical properties of samples. The core function of the TD-NMR Minispec is to perform NMR analysis on a wide range of materials.
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5 protocols using td nmr minispec
1
Investigating DIZE's Effects on Diet-Induced Obesity in Rats
2
Quantifying Body Composition in Mice
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Body composition was assessed using TD-NMR in restrained but fully conscious mice (TD-NMR Minispec, Bruker Optics, The Woodlands, TX). The MiniSpec identifies three components of body composition (fat, free body fluid, and lean tissue in grams) by acquiring and analysing TD-NMR signals from all protons in the sample area. Scans were acquired by placing mice into a cylindrical restrainer (90-mm diameter and ∼250-mm length), fitted with a screw top that tightens to the length of the mice. The restrainer was then inserted into the analyser. The total scan time was ∼1 min, and the average of two scans was used as the final measurement.
3
Body Composition Assessment in Rats
4
Metabolic Phenotyping of Lean Mice
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All animal studies were conducted in accordance with IACUC and CUMC standards and were IACUC approved under protocol AC-AAAH1203. Ten week old male C57BL/6J mice from Jackson Laboratories were used (stock #000664). Body weights, fat mass, and lean mass were measured at 8 and 10 weeks of age. Fat mass and lean mass was measured using the Bruker Minispec TD NMR. Mice were fed Research Diets PicoLab Rodent Diet 20 (#5053) with a fat content of 4.5% of calories. Blood was collected by submandibular bleeding and used for both blood glucose and plasma leptin measurements. Blood glucose was measured following an overnight (16 hours) fast and following 4 hours of ad lib re-feeding using a FreeStyle Lite blood glucose meter and strips (accurate range 30–372 mg/dL). Food intake during the 4-hour refeeding period was measured on a per-cage basis. Using an Acculab Vicon VIC212 scale; five similarly sized chow pellets were weighed and placed in the cage for the feeding period; and were weighed again at its completion; the difference in these numbers is the cumulative cage food intake for the 4 hour period. Statistical analysis was done using Prism 7 from GraphPad Software, Inc.
5
Metabolic Phenotyping of Lean Mice
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