L glu

Manufactured by Thermo Fisher Scientific

Sourced in United States, Italy, United Kingdom

L-Glu is a laboratory reagent used for the measurement of glutamic acid levels. It is a colorless, crystalline powder that is soluble in water and other polar solvents. L-Glu is commonly used in biochemical and enzymatic assays to quantify glutamic acid concentrations in various samples, such as biological fluids, food, and environmental samples.

Automatically generated - may contain errors

Lab products found in correlation

Spelling variants of this product

L-glutamine (L-Glu), (3 citations)

33 protocols using l glu

Isolation and Culture of Primary Hippocampal Neurons

Check if the same lab product or an alternative is used in the 5 most similar protocols

Primary neurons were isolated from the hippocampus of E16-E18 mice and seeded in cell climbing slices (Corning, 354087) or plates that were coated with poly-D-lysine (5 μg/mL, Sigma, P0899-10). Approximately 1 × 10⁵cells per well were seeded for a slice, while 1.5 million cells per well were seeded for a 6-well-plate. After growing in the plating medium for 4 h, which consisted of MEM (Gibco,11095-080), 10% FBS (Gibco,10091-148), 1% L-Glu (Gibco, 5030-149), 1% sodium pyruvate (Gibco, 11360-070), and 0.45% D-glucose (Amresco, 0188), the medium was changed to a maintaining medium that consisted of neurobasal (Gibco, 21103-049), 0.25% L-Glu (Gibco, 25030-149), 0.125% GlutaMax (Thermo, 35050061), and 2% B27 (Gibco, 17504-044). The medium was renewed half of the liquid volume every 3 d.

Cheng X.J., Guan F.L., Li Q., Dai G., Li H.F, & Li X.K. (2020). AlCl3 exposure regulates neuronal development by modulating DNA modification. World Journal of Stem Cells, 12(11), 1354-1365.

+ Open protocol

+ Expand

Culturing Human Lung Cell Lines

Check if the same lab product or an alternative is used in the 5 most similar protocols

Human alveolar basal epithelial (A549) cells were obtained from ATCC and cultured in Roswell Park Memorial Institute 1,640 (RPMI, Gibco, Life Technologies, Italy) medium supplemented with 10% fetal bovine serum (FBS, Gibco, Life Technologies, Italy), 1% L-Glutamine (L-Glu, Gibco, Life Technologies, Italy) and 1% penicillin/streptomycin (P/S, Gibco, Life Technologies, Italy). Human endothelial vein (HEVC) cells were obtained from Biology bank and Cell factory (IRCCS AOU San Martino - IST Italy)and cultured in Dulbecco’s Modified Eagle Medium (DMEM, high glucose, Gibco, Life Technologies, Italy) supplemented with 10% FBS, 2% L-Glu, 1% P/S. Human lung fibroblasts (MRC-5) were cultured in EMEM medium supplemented with 10% FBS and 1% P/S. Cells were maintained in a humidified incubator at 37°C and 5% CO₂ on cell culture flasks.

Licciardello M., Traldi C., Cicolini M., Bertana V., Marasso S.L., Cocuzza M., Tonda-Turo C, & Ciardelli G. (2024). A miniaturized multicellular platform to mimic the 3D structure of the alveolar-capillary barrier. Frontiers in Bioengineering and Biotechnology, 12, 1346660.

+ Open protocol

+ Expand

Cell Culture Protocol for Kasumi-1, HEK 293T, and KO52

Check if the same lab product or an alternative is used in the 5 most similar protocols

Kasumi-1 and HEK 293T cells were purchased from the Deutsche Sammlung von Mikroorganismen und Zellkulturen. KO52 were obtained from the Japanese Collection of Research Bioresources Cell Bank. Kasumi-1 cells were cultured in RPMI-1640 (Sigma-Aldrich) supplemented with 10% fetal calf serum (FCS) (Gibco), 5% Penicillin-Streptomycin (Pen-Strep) (Sigma-Aldrich), 5% L-Glutamine (L-Glu) (Sigma-Aldrich). HEK 293T cells were cultured in DMEM supplemented with 10% FCS (Gibco), 5% Pen-Strep, 5% L-Glu. KO52 were cultured in Alpha MEM (Lonza) supplemented with 10% FCS (Gibco), 5% Pen-Strep, 5% L-Glu.

Adamo A., Chin P., Keane P., Assi S.A., Potluri S., Kellaway S.G., Coleman D., Ames L., Ptasinska A., Delwel H.R., Cockerill P.N, & Bonifer C. (2022). Identification and interrogation of the gene regulatory network of CEBPA-double mutant acute myeloid leukemia. Leukemia, 37(1), 102-112.

+ Open protocol

+ Expand

Culturing Cortical and Hippocampal Neurons

Check if the same lab product or an alternative is used in the 5 most similar protocols

The fetal cortex was dissected from the brain of E15 mice pups and treated with 0.25% trypsin at 37°C for 30 min. Around 150,000 cortical neurons were plated onto a cell climbing slice (Corning) coated with poly-D-lysine (5 μg/mL, Sigma, P0899-10). Neurons were cultured with DMEM (Gibco, 11095-080) supplemented with 10% FBS (Gibco, 10091-148), 1% L-Glu (Gibco, 5030-149), and 1% sodium pyruvate (Gibco, 11360-070) for 4 h. The medium was replaced with neurobasal medium (Gibco, 21103-049) supplemented with 2% B27 (Gibco, 17504-044), 0.25% L-Glu (Gibco, 25030-149), and 0.125% Glutamax (Thermo, 35050061).
Neonatal mice (postnatal day 1–3) were sacrificed, and cortical and hippocampi regions were dissected out under a microscope. The tissues were digested with 0.25% trypsin (Gibco, 25200072) for 25 min at 37°C to dissociate into single cell suspensions. About 1 × 10⁷ cells were plated onto one poly-D-lysine-coated T25 culture flask with DMEM medium supplemented with 10% FBS, 1% antibiotic-antimycotic, and 2 mM L-Glutamine, and the medium was replaced every 2 days. After culture for 7–10 days, samples were put on a shaker (240 rpm) for 12 h at 37°C, and the medium was completely replaced with fresh culture medium.

Li Q., Chen J., Liang F., Zhang J., Qu W., Huang X., Cheng X., Zhao X., Yang Z., Xu S, & Li X. (2021). RYBP modulates embryonic neurogenesis involving the Notch signaling pathway in a PRC1-independent pattern. Stem Cell Reports, 16(12), 2988-3004.

+ Open protocol

+ Expand

Cell Culture Conditions for Kasumi-1, HEK293T, and KO52 Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Kasumi-1 and HEK293T cells were purchased from the Deutsche Sammlung von Mikroorganismen und Zellkulturen. KO52 were obtained from the Japanese Collection of Research Bioresources Cell Bank. Kasumi-1 cells were cultured in RPMI-1640 (Sigma-Aldrich) supplemented with 10% fetal calf serum (FCS) (Gibco), 5% Penicillin-Streptomycin (Pen-Strep) (Sigma-Aldrich), 5% L-Glutamine (L-Glu) (Sigma-Aldrich). HEK 293T cells were cultured in DMEM supplemented with 10% FCS (Gibco), 5% Pen-Strep, 5% L-Glu. KO52 were cultured in Alpha MEM (Lonza) supplemented with 10% FCS (Gibco), 5% Pen-Strep, 5% L-Glu.

+ Open protocol

+ Expand

Directed Differentiation of iPSCs

Check if the same lab product or an alternative is used in the 5 most similar protocols

iPSC colonies were detached using colagenase type IV (Gibco) for 30 min at 37°C, washed and centrifuged at 200× g, resuspended in differentiation media composed by KO-DMEM (Gibco) supplemented with 20% non-heat-inactivated FBS (Biowhitaker), 1% NEAA (Lonza; Biowhitaker), L-Glu (1 mM; Invitrogen), β-mercaptoethanol (0.1 mM; Gibco) and hrBMP4 (0.5 ng/ml; Prepotech) and plated in ultra-low attachment plates (Costar). After 2 days, media were replaced by Stempro 34 (Invitrogen) supplemented with 0.5% pen/streptomicin, L-Glu (2 mM; Invitrogen), MTG (40 mM; Sigma), ascorbic acid (50 μg/ml; Invitrogen), hrSCF, hrFlt3 ligand and TPO (100 ng/ml; EuroBioSciences), hrIL3 (10 ng/ml; Biosource), hrIL6 (10 ng/ml; Prepotech), hrBMP4 (50 ng/ml; Prepotech), Wnt11 (200 ng/ml; R&D), and rhVEGF (5 ng/ml; Prepotech). Media were changed every 3–4 days. At day 7, media were replaced by fresh media where rhWnt-11 was substituted by rhWnt-3a (200 ng/ml; R&D). Media were changed every 3–4 days. At day 14 and 21, immunophenotypic analysis of the differentiated cells was performed by flow cytometry, and colony-forming unit assays were conducted (See Supplementary Methods).

Rio P., Baños R., Lombardo A., Quintana-Bustamante O., Alvarez L., Garate Z., Genovese P., Almarza E., Valeri A., Díez B., Navarro S., Torres Y., Trujillo J.P., Murillas R., Segovia J.C., Samper E., Surralles J., Gregory P.D., Holmes M.C., Naldini L, & Bueren J.A. (2014). Targeted gene therapy and cell reprogramming in Fanconi anemia. EMBO Molecular Medicine, 6(6), 835-848.

+ Open protocol

+ Expand

FPR2 Receptor Activation Assay in HEK-293 Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

HEK-293-transfected
and wt cell lines were cultured in Dulbecco’s modified Eagle’s
medium (DMEM Glutamax, 4 mM l-glu and 4.50 g/L glucose—Gibco,
Ireland) supplemented with heat-inactivated (30 min at 56C) 10% (v/v)
FBS (Invitrogen, U.K.), 50 U penicillin, and 50 μg of streptomycin
(Invitrogen, U.K.). As stably double-transfected FPR2⁺/Gα_q⁺ HEK-293 cells were resistant to Geneticin and
Hygromycin, these were used as selective antibiotics (500 and 100
μg/mL, respectively) to maintain the stable double transfection
with the ALX/FPR2 receptor and the Gα_q subunit coupled
to it. Immediately prior to calcium measurement, fluorescently labeled
cells were treated with 10^{–11/–9/–7}M LXA₄1 or sLXms using an injection system
coupled to the fluorescence reader.

de Gaetano M., Tighe C., Gahan K., Zanetti A., Chen J., Newson J., Cacace A., Marai M., Gaffney A., Brennan E., Kantharidis P., Cooper M.E., Leroy X., Perretti M., Gilroy D., Godson C, & Guiry P.J. (2021). Asymmetric Synthesis and Biological Screening of Quinoxaline-Containing Synthetic Lipoxin A4 Mimetics (QNX-sLXms). Journal of Medicinal Chemistry, 64(13), 9193-9216.

+ Open protocol

+ Expand

Cell Culture Conditions for Viral Studies

Check if the same lab product or an alternative is used in the 5 most similar protocols

Madin-Darby canine Kidney (MDCK) cells (ATCC) were cultured in Eagle’s minimal essential medium (EMEM, Lonza Benelux BV, Breda, the Netherlands) supplemented with 10% foetal bovine serum (FBS) (Greiner), 100 U ml⁻¹ penicillin (PEN, Lonza), 100 U ml⁻¹ streptomycin (STR, Lonza), 2 mM L-glutamine (L-glu, Lonza), 1.5 mg ml⁻¹ sodium bicarbonate (NaHCO₃, Lonza), 10 mM Hepes (Lonza) and 1X non-essential amino acids (NEAA, Lonza). 293T cells (ATCC) were cultured in Dulbecco modified Eagle’s medium (DMEM, Lonza) supplemented with 10% FBS, 100 U ml⁻¹ PEN, 100 U ml⁻¹ SRT, 2mM L-glu, 1 mM sodium pyruvate (Gibco) and 1X NEAA. Human airway epithelia reconstituted in vitro (MucilAir^TM, EP02MP) were purchased from Epithelix Sàrl (Switzerland).

Richard M., van den Brand J.M., Bestebroer T.M., Lexmond P., de Meulder D., Fouchier R.A., Lowen A.C, & Herfst S. (2020). Influenza A viruses are transmitted via the air from the nasal respiratory epithelium of ferrets. Nature Communications, 11, 766.

+ Open protocol

+ Expand

Culturing Squamous Cell Carcinoma Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

SCC-13 cells (Cellosaurus:RRID:CVCL_4029) were derived from skin squamous cell carcinoma (NCIt: C4819), SCC 011 (Cellosaurus: RRID:CVCL_5986) were derived from laryngeal squamous cell carcinoma (NCIt: C4044) and SCC 022 (Cellosaurus: RRID:CVCL_5991) were derived from laryngeal squamous cell carcinoma (NCIt: C4044). All the cells were mycoplasma free. SCC-13 and SCC D2KO cells were cultured in keratinocyte-SFM (KSFM 1×) serum-free medium [+] L-Glu (Gibco Life Technologies) with bovine pituitary extract (30 µg/ml) and human recombinant epidermal growth factor (EGF) protein (0.24 ng/ml). SCC D3KO cells were cultured in KSFM with 4% Ca²⁺-chelated charcoal-stripped fetal bovine serum (FBS) and EGF human recombinant (Gibco Life Technologies). All transient transfections were performed using Lipofectamine 2000 (Life Technologies) according to the manufacturer’s instructions.

Miro C., Di Cicco E., Ambrosio R., Mancino G., Di Girolamo D., Cicatiello A.G., Sagliocchi S., Nappi A., De Stefano M.A., Luongo C., Antonini D., Visconte F., Varricchio S., Ilardi G., Del Vecchio L., Staibano S., Boelen A., Blanpain C., Missero C., Salvatore D, & Dentice M. (2019). Thyroid hormone induces progression and invasiveness of squamous cell carcinomas by promoting a ZEB-1/E-cadherin switch. Nature Communications, 10, 5410.

+ Open protocol

+ Expand

Culturing Various Cell Lines

Check if the same lab product or an alternative is used in the 5 most similar protocols

Human embryonic kidney cells GripTite 293 MSR (293GT, Life Technologies, Carlsbad, CA, USA) and Madin Darby Canine Kidney (MDCK, ATCC, Manassas, VA, USA) cells were grown in either Dulbeco’s minimum essential medium (DMEM, HyClone, GE Healthcare Life Sciences, Logan, Utah, USA) or minimum essential medium (MEM, HyClone) supplemented with 5% fetal bovine serum (FBS, HyClone) and 1X L-Glutamine (L-Glu, Gibco, Life Technologies, Grand Island, NY, USA), respectively. Human lung epithelial cells (A549, ATCC) were cultured in DMEM supplemented with 5% FBS and L-Glu. Mouse lung cells (KLN 205, ATCC) were cultured in MEM supplemented with 10% FBS and L-Glu. Chicken fibroblast cells (DF-1, ATCC) were cultured in DMEM supplemented with 10% FBS and L-Glu. All cells were grown in a humidified incubator with 5% CO_2, at either 37 °C (293GT, MDCK, A549, and KLN 205 cells) or 39 °C (DF-1 cells).

Chan M., Leung A., Hisanaga T., Pickering B., Griffin B.D., Vendramelli R., Tailor N., Wong G., Bi Y., Babiuk S., Berhane Y, & Kobasa D. (2020). H7N9 Influenza Virus Containing a Polybasic HA Cleavage Site Requires Minimal Host Adaptation to Obtain a Highly Pathogenic Disease Phenotype in Mice. Viruses, 12(1), 65.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!