An HPLC 1100 equipped with a VWD detector (Agilent Technologies, Palo Alto, CA, USA) was used. The reversed stationary phase employed was a Synergy Hydro-RP-80A 5 μm (250 × 4.6 mm i.d., Phenomenex, Castel Maggiore, Bologna, Italy) with a pre-column Gemini C18 5 μm (4 × 2 mm i.d., Phenomenex, Castel Maggiore, Bologna, Italy). The separation was conducted in an isocratic mode using water/orthophosphoric acid (0.1%) as the mobile phase. The flow was maintained at 0.7 mL/min and sample injection was 5 μL. Wine samples were 2-folds diluted and filtered (0.2 μm RC syringe filters, Phenomenex) before the HPLC analysis. Absorbance was recorded at 210 nm.
Rc syringe filters
Manufactured by Phenomenex
Sourced in United States
RC syringe filters are designed for sample preparation and filtration in laboratory environments. They are composed of regenerated cellulose membrane and provide efficient removal of particles and particulates from liquid samples prior to analysis or further processing.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using rc syringe filters
1
HPLC Analysis of Wine Compounds
2
Wastewater Preconcentration and Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The sample preparation protocol involved clean-up and 4000x preconcentration on an Atlantic HLB-M Disk, using a HORIZON SPE-DEX 4790 (USA) with 47 mm disk holder. Conditioning and extraction programs used for the preparation of the wastewater samples can be found in the SI (Table SC.2). The extract was evaporated using a gentle stream of nitrogen and was reconstituted with 250 μl of 50:50 methanol:water mixture for instrumental analysis. Before analysis, extracts were filtered through RC syringe filters of 4 mm diameter and 0.2 μm pore size (Phenomenex, USA). See SI (SC.1) for information on chemicals and reagents.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!