Log In Sign up
The largest database of trusted experimental protocols

Ab103790

Manufactured by Abcam
Visit Supplier
Sourced in United States

Ab103790 is a recombinant protein product. It is a tool for research purposes.

Automatically generated - may contain errors

Lab products found in correlation

Hoechst 33342, by Thermo Fisher Scientific (2 mentions) Ab7842, by Abcam (2 mentions) Ab30477, by Abcam (2 mentions) Ab26405, by Abcam (2 mentions) Ab66506, by R&D Systems (2 mentions) Ab15160, by BioGenex (2 mentions) Ab20592, by Abcam (2 mentions) Ab10988, by Abcam (2 mentions) Af2419, by Abcam (2 mentions) Mu363 uc, by Abcam (2 mentions) Tsa cy3 kit, by Akoya Biosciences (2 mentions) Alexa fluor 488 donkey anti rabbit igg h l, by Thermo Fisher Scientific (1 mentions) Alexa fluor 568 donkey anti mouse igg, by Thermo Fisher Scientific (1 mentions) Ab6995, by Abcam (1 mentions) Ab8055, by Abcam (1 mentions) Cm3050, by Leica (1 mentions)

4 protocols using ab103790

1

Multiparametric Islet Immunophenotyping

Check if the same lab product or an alternative is used in the 5 most similar protocols
Immunohistochemistry of frozen or paraffin-embedded sections of pancreas, human islets, and human islet-like organoids (HILOs) was performed with antibodies to insulin (1/100, Abcam ab7842), c-peptide (1/100, Abcam ab30477), glucagon (1/100, Abcam ab10988), somatostatin (1/100, Abcam ab103790), pancreatic polypeptyde (1/100, Abcam, ab113694), NKX2–2 (1/100, DSHB, 74.5A5), NKX6–1 (1/100, DSHB, F55A12), MAFA (1/100, Abcam, ab26405), MAFB (1/100, Abcam, ab66506), PDX-1 (1/100, R&D, AF2419), CHGA (1/100, Abcam, ab15160), SYNAPTOPHYSIN (1/100, Biogenex, MU363-UC) and PD-L1 (1/100, Abcam, ab20592). For MAFA and MAFB, signal amplification was performed using TSA-Cy3 kit (Akoya Biosciences, SAT704A001EA). Secondary antibodies were coupled to Alexa 568, 647 (Life Technologies) and visualized by confocal microscopy (ZEISS) or fluorescence microscopy. Hoechst 33342 (Thermo Scientific, 62249, 1μg/ml final concentration) was used for nuclear staining.
Yoshihara E., O’Connor C., Gasser E., Wei Z., Oh T.G., Tseng T.W., Wang D., Cayabyab F., Dai Y., Yu R.T., Liddle C., Atkins A.R., Downes M, & Evans R.M. (2020). Immune evasive human islet-like organoids ameliorate diabetes. Nature, 586(7830), 606-611.
+ Open protocol
+ Expand
2

Pancreatic Cell Immunohistochemistry

Check if the same lab product or an alternative is used in the 5 most similar protocols
Whole 5% paraformaldehyde-fixed and paraffin-embedded samples of pancreas from Wistar and Goto-Kakizaki rats, typically of ages of 31, 52, and 79 weeks, were investigated using either monoclonal antibody mouse anti-insulin ab6995 (ABCAM, Cambridge, UK), diluted 1 : 100, or polyclonal antibodies such as rabbit anti-glucagon ab8055 (ABCAM), diluted 1 : 100, rabbit anti-somatostatin ab103790 (ABCAM), diluted 1 : 500, and rabbit anti-pancreatic polypeptide PA1-36141 (Pierce Biotechnology, Rockford, IL), diluted 1 : 1000. Secondary antibodies (Life Technologies), diluted 1 : 1000, were Alexa Fluor 568 Donkey anti-Mouse IgG, A10037 for red imaging, and Alexa Fluor 488 Donkey anti-rabbit IgG (H + L), A21206, for green imaging. Immunohistochemical samples were viewed by a motorized inverted fluorescence microscope Olympus IX-81 and Cell F software.
Alán L., Olejár T., Cahová M., Zelenka J., Berková Z., Smětáková M., Saudek F., Matěj R, & Ježek P. (2015). Delta Cell Hyperplasia in Adult Goto-Kakizaki (GK/MolTac) Diabetic Rats. Journal of Diabetes Research, 2015, 385395.
+ Open protocol
+ Expand
3

Somatostatin Immunohistochemistry in GIN Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols
Anesthetized GIN mice were perfused with 4% paraformaldehyde (in phosphate-buffered saline, pH 7.4). Next, the brains were post-fixed for 24 h. Brain slices (50 μm) were prepared with a cryostat (CM3050, Leica). Brain sections were blocked with 3% donkey serum and 1% bovine serum albumin in Tris-buffered saline containing 0.5% Triton X-100 for 1 h at room temperature. The brain sections were then probed with mouse anti-SST (1:200, #ab103790, Abcam, Cambridge, MA, USA) at 4 °C for 72 h and visualized using an Alexa Fluor 647-conjugated donkey anti-mouse secondary antibody (1:200, #711605152, Jackson ImmunoResearch, West Grove, PA, USA).
Pan G., Yang J.M., Hu X.Y, & Li X.M. (2016). Postnatal development of the electrophysiological properties of somatostatin interneurons in the anterior cingulate cortex of mice. Scientific Reports, 6, 28137.
+ Open protocol
+ Expand
4

Multiparametric Islet Immunophenotyping

Check if the same lab product or an alternative is used in the 5 most similar protocols
Immunohistochemistry of frozen or paraffin-embedded sections of pancreas, human islets, and human islet-like organoids (HILOs) was performed with antibodies to insulin (1/100, Abcam ab7842), c-peptide (1/100, Abcam ab30477), glucagon (1/100, Abcam ab10988), somatostatin (1/100, Abcam ab103790), pancreatic polypeptyde (1/100, Abcam, ab113694), NKX2–2 (1/100, DSHB, 74.5A5), NKX6–1 (1/100, DSHB, F55A12), MAFA (1/100, Abcam, ab26405), MAFB (1/100, Abcam, ab66506), PDX-1 (1/100, R&D, AF2419), CHGA (1/100, Abcam, ab15160), SYNAPTOPHYSIN (1/100, Biogenex, MU363-UC) and PD-L1 (1/100, Abcam, ab20592). For MAFA and MAFB, signal amplification was performed using TSA-Cy3 kit (Akoya Biosciences, SAT704A001EA). Secondary antibodies were coupled to Alexa 568, 647 (Life Technologies) and visualized by confocal microscopy (ZEISS) or fluorescence microscopy. Hoechst 33342 (Thermo Scientific, 62249, 1μg/ml final concentration) was used for nuclear staining.
Yoshihara E., O’Connor C., Gasser E., Wei Z., Oh T.G., Tseng T.W., Wang D., Cayabyab F., Dai Y., Yu R.T., Liddle C., Atkins A.R., Downes M, & Evans R.M. (2020). Immune evasive human islet-like organoids ameliorate diabetes. Nature, 586(7830), 606-611.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!