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Sh30396

Manufactured by Cytiva
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Sourced in United States

The SH30396.03 is a laboratory equipment product. It is a type of specialized apparatus designed for use in research and scientific settings. The core function of this equipment is to facilitate specific laboratory processes or analyses, but a detailed description without interpretation or extrapolation is not available.

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Lab products found in correlation

Dulbecco modified eagle medium (dmem), by Fujifilm (2 mentions) Penicillin streptomycin amphotericin b suspension 100 antibiotic antimycotic solution, by Fujifilm (2 mentions) Caski, by RIKEN BioResource Center (1 mentions) Rpmi 1640 medium, by BioConcept (1 mentions) Antibiotic antimycotic solution, by Fujifilm (1 mentions) Fetal bovine serum (fbs), by Cytiva (1 mentions) Glutamax, by Thermo Fisher Scientific (1 mentions) Crl 1573, by American Type Culture Collection (1 mentions) Hek293ft cells, by Thermo Fisher Scientific (1 mentions) Penicillin streptomycin solution, by Thermo Fisher Scientific (1 mentions) Ccl 226, by American Type Culture Collection (1 mentions) Hela ccl 2, by American Type Culture Collection (1 mentions) 2 mercaptoethanol, by Thermo Fisher Scientific (1 mentions) Chicken serum, by Thermo Fisher Scientific (1 mentions) D glucose, by Fujifilm (1 mentions) Sodium carbonate, by Fujifilm (1 mentions)

6 protocols using sh30396

1

Culturing Human Cervical Cancer Cells

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CaSki and C33A human cervical cancer cell lines were purchased from the Bioresource Collection and Research Center (BCRC, Hsinchu, Taiwan; derived from ATCC CRM-1550TM and ATCC CRM-HTB-31). CaSki cells were cultured in an RPMI 1640 medium (1-41P05-K, BioConcept, Amimed, Allschwil, Switzerland), and C33A cells in Eagle’s Minimum Essential Medium (SH30024.02, MEM, Cytiva, Marlborough, MA, USA) supplemented with 10% fetal bovine serum (SH30396.03, FBS, Cytiva) at 37 °C in a humidified incubator with 5% CO2.
Chen Y.H., Wu J.X., Yang S.F., Chen M.L., Chen T.H, & Hsiao Y.H. (2021). Metformin Potentiates the Anticancer Effect of Everolimus on Cervical Cancer In Vitro and In Vivo. Cancers, 13(18), 4612.
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2

Fibroblast Stimulation by Poly I:C

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After preculture for 48 h, fibroblasts were exposed to 5 μg/mL or 50 μg/mL of poly I:C (polyinosinic-polycytidylic acid sodium salt) (4287/10; R&D Systems, Minneapolis, MN, USA). Poly I:C is a mixture of long and short reagents. During preculture and poly I:C exposure, the cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM; 043–30085; FUJIFILM Wako Pure Chemical Industries) containing 10% FBS (SH30396.03; Cytiva) and 1% penicillin-streptomycin-amphotericin B suspension (×100) (Antibiotic-Antimycotic Solution) (161–23181; FUJIFILM Wako Pure Chemical Industries). Cells were cultured at 37°C in 5% CO2.
Katayama M., Fukuda T., Kato N., Nagamine T., Nakaya Y., Nakajima N, & Onuma M. (2023). Cultured fibroblasts of the Okinawa rail present delayed innate immune response compared to that of chicken. PLOS ONE, 18(8), e0290436.
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3

Poly I:C Exposure on Organoid Size

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Before poly:IC exposure, we cut the formed organoid with ophthalmologic scissors to approximately one-fourth the size under a stereoscopic microscope due to the reduction of variety of sizes. The organoids were exposed to 5 μg/mL or 50 μg/mL poly I:C (polyinosinic-polycytidylic acid sodium salt) (4287/10; R&D Systems, Minneapolis, MN) for 6 h. We randomly chose nine-pieces from each dish (exposure of 0 μg/mL, 5 μg/mL, 50 μg/mL poly:IC) from a total of 36 pieces of organoids. During preculture and poly I:C exposure, the cells were cultured in DMEM. The DMEM (043–30085; FUJIFILM Wako Pure Chemical Industries) medium contained 10% FBS (SH30396.03; Cytiva) and 1% Penicillin-Streptomycin-Amphotericin B Suspension (×100) (Antibiotic-Antimycotic Solution) (161–23181; FUJIFILM Wako Pure Chemical Industries). Cells were cultured at 37°C in 5% CO2.
Katayama M., Onuma M., Kato N., Nakajima N, & Fukuda T. (2023). Organoids containing neural-like cells derived from chicken iPSCs respond to poly:IC through the RLR family. PLOS ONE, 18(5), e0285356.
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4

Mammalian Cell Culture Protocol

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Mammalian cell lines were cultured in a humidified incubator maintained at 37 °C with 5% CO2. Cell media were based on the Dulbecco’s Modified Eagle’s Medium (DMEM), pH 7.0–7.4, containing high glucose and sodium pyruvate and without L-glutamine (SH30285.01, Cytiva or similar), used with supplements as indicated below. FBS was typically Cytiva Characterized Fetal Bovine Serum, Canadian Origin (SH30396.03, Cytiva), or Corning Regular Fetal Bovine Serum (35010CV, Corning). HEK293-FT cells (R70007, ThermoFisher) were cultured in DMEM with 10% FBS and supplemented with 1× nonessential amino acids (25025CI, Corning) and 1× GlutaMAX (3505006, ThermoFisher). HeLa cells (CCL-2, ATCC), C3H/10T1/2 cells (CCL-226, ATCC), HEK293 (CRL-1573, ATCC) and U-2 OS cells (92022711-1VL, Sigma-Aldrich) were cultured using the same medium but with added 1× penicillin-streptomycin solution (15140122, ThermoFisher).
, & Einterz E.M. (2001). Sooner or later, everyone is going to die anyway. CMAJ: Canadian Medical Association Journal, 165(12), 1604.
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5

Cell Culture Maintenance and Characterization

Cited 1 time
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HEK293T/17 (CRL-11268, ATCC, VA, USA) and HeLa (CCL-2, ATCC, VA, USA) cell lines were maintained in Dulbecco’s modified Eagle medium (DMEM; 11995-073, Thermo Fisher, Waltham, MA, USA) supplemented with 1% minimal essential medium non-essential amino acids solution (11140-050, Thermo Fisher, Waltham, MA, USA) and 10% fetal bovine serum (SH30396.03, Cytiva, Utah, USA). Cell lines were purchased from the Duke Cell Culture facility where they underwent STR authentication. Cell lines were tested regularly for mycoplasma infection by PCR (Dreolini et al., 2020 (link)). Cell lines were kept under 25 passages after thawing.
Billman Z.P., Kovacs S.B., Wei B., Kang K., Cissé O.H, & Miao E.A. (2024). Caspase-1 activates gasdermin A in non-mammals. eLife, 12, RP92362.
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6

Fibroblast Culture from Avian Species

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We used KAv-1 medium to obtain and culture chicken, Okinawa rail, domestic duck, and whooper swan fibroblasts. KAv‐1 is based on α‐MEM and contains 5% FBS (SH30396.03; Cytiva, Marlborough, MA, USA), 5% chicken serum (16110082; Thermo Fisher, Waltham, MA, USA), 0.1% D-glucose (041–00595; FUJIFILM Wako Pure Chemical Industries, Osaka, Japan), 0.4 mM calcium chloride, 10 mM EPPS (348–03192; FUJIFILM Wako Pure Chemical Industries), 0.11% sodium carbonate (199–01585; FUJIFILM Wako Pure Chemical Industries), 55 μM 2-mercaptoethanol (21985–023; Thermo Fisher, Waltham, MA, USA), and 1% penicillin-streptomycin-amphotericin B suspension (×100) (Antibiotic-Antimycotic Solution) (161–23181; FUJIFILM Wako Pure Chemical Industries). Cells were cultured at 37°C in 5% CO2.
Katayama M., Fukuda T., Kato N., Nagamine T., Nakaya Y., Nakajima N, & Onuma M. (2023). Cultured fibroblasts of the Okinawa rail present delayed innate immune response compared to that of chicken. PLOS ONE, 18(8), e0290436.
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