To investigate lung metastasis in the animal model, IF and HE (hematoxylin and eosin) staining assays were performed on poly-L -lysine-coated slides. The slides were incubated with FITC-labeled anti-Vimentin antibodies for 2 h at room temperature, washed twice with phosphate-buffered saline, and incubated with secondary antibodies for an additional 1 h in a moist chamber at room temperature. The slides were stained with Hoechst 33258 for nuclei location and examined with a Leica DMI3000 B Fluorescence Microscopy Imaging System (Leica Microsystems, Wetzlar, Germany). To enhance the color contrast of Vimentin and nuclei location, The FITC signal was displayed with green color, and nuclei location was displayed with red color.
Dmi3000 b fluorescence microscopy imaging system
Manufactured by Leica
Sourced in Germany
The Leica DMI3000 B is a fluorescence microscopy imaging system designed for advanced research applications. It features a high-performance illumination system, optimized optics, and comprehensive software integration to enable detailed analysis of fluorescently labeled samples.
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Lab products found in correlation
2 protocols using dmi3000 b fluorescence microscopy imaging system
1
Visualizing Lung Metastasis in Animal Model
2
Fluorescent Visualization of Vimentin in Lung Metastasis
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To investigate lung metastasis in the animal model, IF staining assay were performed on poly-L-lysine-coated slides. The slides were incubated with FITC-labeled anti-Vimentin antibodies for two h at room temperature, washed twice with phosphate-buffered saline, and incubated with secondary antibodies for an additional one h in a moist chamber at room temperature. The slides were stained with Hoechst 33258 for nuclei location and examined with a Leica DMI3000 B Fluorescence Microscopy Imaging System (Leica Microsystems, Wetzlar, Germany). To enhance the colour contrast of Vimentin and nuclei location, The FITC signal was displayed with green colour, and nuclei location was displayed with red colour.
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