The following commercially available antibodies were used: goat polyclonal anti-EGFP (Rockland), chicken polyclonal anti-EGFP (Aves Labs), rabbit polyclonal anti-RFP (Abcam), mouse monoclonal anti-HA (clone 16B12; Covance), mouse monoclonal anti-GAD67 (clone 1G10.2; Millipore), guinea pig polyclonal anti-VGLUT1 (Millipore), mouse monoclonal anti-gephyrin (clone 3B11; Synaptic Systems), rabbit polyclonal anti-VGAT (Synaptic Systems), rabbit polyclonal anti-GABAARγ2 (Synaptic Systems), mouse monoclonal anti-PSD-95 (clone K28/43; Neuromab), mouse monoclonal anti-gephyrin (clone 3B11; Synaptic Systems), and rabbit polyclonal anti-ARF6 (Abcam). Rabbit polyclonal anti-IQSEC3 (JK079) [29 (link)] and guinea pig polyclonal anti-IQSEC3/SynArfGEF (a gift from Dr. Hiroyuki Sakagami) [35 (link)] antibodies were previously described.
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