Prothionamide

N-vinyl-2-pyrrolidone (VP), 2,2’-azobisisobutyronitrile (AIBN), 1,4-dioxane were obtained from Acros (Geel, Belgium). Prothionamide, stearoyl chloride, maleimide, potassium tert-butylate, dimethylsulfoxide (DMSO), Fetal bovine serum (FBS), phosphate buffer saline (PBS), and all other chemicals were obtained from Sigma-Aldrich (Saint Louis, MO, USA) unless otherwise specified. All solvents and components of buffer solutions were of analytical grade and used as received. Milli-Q Plus System (Merck KGaA, Darmstadt, Germany) was used for distilled–deionized water preparation.
Human colorectal carcinoma HCT116 cells, breast adenocarcinoma MCF-7 cells, normal keratinocytes HaCaT and skin fibroblasts BJ-5ta were from ATCC (Washington, WA, USA); human colorectal adenocarcinoma HT-29 was kindly provided by Institute of Cytology, Russian Academy of Sciences (St. Petersburg, Russia). Cell culture media (DMEM, RPMI1640), 0.05% trypsin-EDTA solution, and phosphate-buffered saline tablets were from PanEco (Moscow, Russia). Fetal bovine serum was from HyClone (Cytiva, Marlborough, MA, USA). MTT reagent was from Sigma-Aldrich (Saint Louis, MO, USA). Cyclic RGD-peptide, namely cyclo-RGDfK(TPP) for generation of multicellular tumor spheroids was kindly provided by Prof. Burov (St-Petersburg, Russia).