Shear stress experiments were performed using a commercially available shear stress system (Quad fluidic unit, Ibidi) according to the manufacturer’s instructions. Cells were seeded onto 1% gelatin-coated μ-slides I 0.4 Luer (Ibidi) and exposed to continuous high LSS (H-LSS; 20dyn/cm2), low LSS (L-LSS; 5dyn/cm2) or OSS (±5dyn/cm2, 2Hz) for 48-96h using a parallel flow system (Ibidi). Cells were pre-conditioned with shear stress for 48h before pharmacologic treatment. Due to the experimental set-up used, MTX treatment was only performed under H-LSS and OSS conditions. Static controls were grown on 6-well plates in parallel to the shear stress experiments.
μ slides 1 0.4 luer
Manufactured by Ibidi
Sourced in Germany
The μ-slides I 0.4 Luer is a specialized lab equipment designed for cell cultivation. It provides a controlled environment for cell growth and analysis. The product features a Luer connector for easy connection to external equipment.
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Lab products found in correlation
2 protocols using μ slides 1 0.4 luer
1
Shear Stress Experiments with Pharmacologic Treatment
2
Endothelial Cell Shear Stress Response
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An in vitro model was used to analyze the effects of combined exposure to plasma and shear stress on HUVEC behavior [Figure 1 ]. μ-Slides I0.4 Luer (80176, ibidi, Gräfelfing, Germany) were used as previously described.[17 (link)]Confluent monolayers of HUVEC were starved for 1 h in FCS-free medium and subsequently incubated with FCS-free medium containing 20% control or patient plasma and 1 IU/ml heparin for 3 h. Thereafter, cells were exposed to 20 dyn/cm2 shear stress for 1 h, to mimic the change of shear stress inherent to the CABG surgery with CPB procedure.
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