HB was obtained from Lhasa, Tibet. Acetic acid ≥98%, propionic acid ≥99%, butyric acid ≥99%, valeric acid ≥99%, and 2-ethylbutyric acid ≥98% were obtained from Aladdin Bio-Chem Technology Co., Ltd., (Shanghai, China). Total glucose oxidase/peroxidase (GOPOD) kit, β-glucan (mixed linkage) kit, total dietary fiber kit, and total starch kit were obtained via Megazyme International Ireland, Ltd. (Bray Co., Wicklow, Ireland). α-amylase from porcine pancreas type VI-B (A3176, 11 units/mg) and pepsin from porcine gastric mucosa (P7125, ≥400 units/mg) were obtained via Sigma Chemical Co. (St. Louis, Mo., USA). Pancreatic enzyme (S10031, trypsin ≥4000 units/g, pancreatic α-amylase ≥7000 units/g, and pancreatic lipase ≥4000 units/g) was obtained via the Shanghai yuanye Bio-Technology Co. (Shanghai, China). Other chemicals and reagents used were of analytical grade.
Pepsin from porcine gastric mucosa p7125
Manufactured by Merck Group
Sourced in United States
Pepsin from porcine gastric mucosa (P7125) is a proteolytic enzyme purified from the gastric mucosa of pigs. It is a widely used lab reagent for the hydrolysis of protein substrates.
Automatically generated - may contain errors
Lab products found in correlation
Dimethyl sulfoxide (dmso), by Xilong (3 mentions)
Trypsin, by Yuanye Bio-Technology (1 mentions)
Pancreatic lipase, by Yuanye Bio-Technology (1 mentions)
Total dietary fiber kit, by Megazyme (1 mentions)
Total starch kit, by Megazyme (1 mentions)
Bca protein assay kit 23225, by Thermo Fisher Scientific (1 mentions)
Ziptip c18 pipette tips, by Merck Group (1 mentions)
Cholesterol, by Sinopharm (1 mentions)
Bile salt, by Merck Group (1 mentions)
P1750, by Merck Group (1 mentions)
1 6 diphenyl 1 3 5 hexatriene, by Merck Group (1 mentions)
3 protocols using pepsin from porcine gastric mucosa p7125
1
Tibetan Barley Compositional Analysis
2
Comparative Protein Digestion Assay
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Pork, beef, chicken, and fish were used. Pork longissimus dorsi muscle (the number of biological replicates, n equals 8, muscle pH 5.83 ± 0.18), beef longissimus dorsi muscle (n = 8, pH 5.46 ± 0.08), chicken pectoralis major muscle (n = 8, pH 5.84 ± 0.05) and fish muscle from silver carps (n = 8, pH 6.67 ± 0.17) were obtained from a commercial meat packing company. Pepsin from porcine gastric mucosa (P7125) and trypsin from porcine pancreas (T7409) were purchased from Sigma Aldrich (USA). BCA protein assay kit (23225) was purchased from Thermo Scientific (USA). The 4–12% Bis‐Tris Criterion precast gels (345‐0125), XT sample buffer (161‐0791), XT MES running buffer (161‐0789), and broad molecular weight calibration marker (Precision Plus Protein Dual Xtra Standard, cat. no. 161–0377, molecular range 2–250 kDa) were purchased from Bio‐Rad (USA). Ultracel‐3 Membrane (Amicon Ultra‐0.5, 3 kDa) (UFC500396) and ZipTip C18 pipette tips (ZTC18S096) were purchased from Millipore (USA).
3
Curcumin Loaded Phospholipid Nanoformulation
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Non-hydrogenated soybean phospholipid (SPC) Lipoid S100 (94% soybean phosphatidylcholine, 3% lysophosphatidylcholine, 0.1% phosphatidylethanolamine, 0.5% Nacyl-phosphatidylethanolamine, 0.1% phosphatidylinositol, 2% water, 0.2% ethanol) was purchased from Lipoid GmbH (Ludwigshafen, Germany). Hydrogenated soybean phospholipid (HSPC, 97% 1,2-distearoyl-sn-glycero-3-phosphocholine) was purchased from Advanced Vehicle Technology Pharmaceutical Co., Ltd (Shanghai, China) . Curcumin (˃ 95%) was obtained from Hebei Food Additive Co., Ltd (Hebei, China) . Cholesterol was purchased from Sinopharm Chemical Reagent Co., Ltd (Shanghai, China) . The fluorescent probe, 1,6diphenyl-1,3,5-hexatriene (DPH, 98%), mucin from porcine stomach (M2378), pepsin from porcine gastric mucosa (P7125), pancreatin from porcine pancreas (P1750) and bile salts were purchased from Sigma-Aldrich (St. Louis MO, USA). Dimethyl sulfoxide (DMSO, 99%) was purchased from Xilong Scientific Co., Ltd (Guangzhou, China) . All other reagents used were analytical grade without further purification.
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