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Cisplatin

Manufactured by Takara Bio
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Sourced in Japan

Cisplatin is a platinum-based chemotherapeutic agent used in the laboratory. It functions as a DNA-binding compound, interfering with cell division and ultimately leading to cell death.

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Lab products found in correlation

Carboplatin, by Takara Bio (2 mentions) Cisplatin, by Merck Group (2 mentions) Carboplatin, by Merck Group (2 mentions) Premix wst 1 reagent, by Takara Bio (1 mentions) Wst 1 reagent, by Takara Bio (1 mentions)

2 protocols using cisplatin

1

Anticancer Drug Cytotoxicity Evaluation

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To evaluate the effect of anticancer drugs, 1 × 104 cells were seeded into 96‐well culture plates and incubated for 24 h at 37°C with 5% CO2. We subsequently added cisplatin (Sigma‐Aldrich, St. Louis, MO, USA) (0, 25, 50, 100, 200 or 400 μM) or carboplatin (Sigma‐Aldrich) (0, 31.25, 62.5, 125, 250 or 500 μM). After 72 h (cisplatin) or 48 h (carboplatin) of incubation, 10 μL of Premix WST‐1 reagent (Takara Bio, Kyoto, Japan) were added to each well. After 1 h incubation, absorbance was measured. The absorbance at 450 nm was subtracted from the background absorbance (690 nm).
Tahara S., Nojima S., Ohshima K., Hori Y., Kurashige M., Wada N., Ikeda J, & Morii E. (2016). S100A4 accelerates the proliferation and invasion of endometrioid carcinoma and is associated with the “MELF” pattern. Cancer Science, 107(9), 1345-1352.
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2

Cytotoxicity Assay of Cisplatin and Carboplatin

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Aliquots of 1 × 104 cells in 96‐well plates were incubated for 24 h. We subsequently added cisplatin (Merck) (0, 1, 2, 4, 8, 16, 32, or 64 μM) or carboplatin (Merck) (0, 15.6, 31.3, 62.5, 125, 250, 500, or 1000 μM). After 48 h (cisplatin) or 72 h (carboplatin) of incubation, we added WST‐1 reagent (Takara Bio) and measured the absorbance using an SH‐9000 laboratory microplate reader (Corona Electric).
Tahara S., Nojima S., Ohshima K., Hori Y., Sato K., Kurashige M., Matsui T., Okuzaki D, & Morii E. (2021). Nicotinamide N‐methyltransferase is related to MELF pattern invasion in endometrioid carcinoma. Cancer Medicine, 10(23), 8630-8640.
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