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Abx pentra 400 analyzer

Manufactured by Horiba
Sourced in France

The ABX Pentra 400 analyzer is a compact and versatile clinical chemistry analyzer designed for routine laboratory testing. It is capable of performing a wide range of biochemical assays on various sample types, including serum, plasma, and urine. The analyzer utilizes advanced spectrophotometric technology to provide accurate and reliable results.

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11 protocols using abx pentra 400 analyzer

1

Quantitative Lipoprotein Profiling by Ultracentrifugation

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Quantification of lipoprotein (LP) particles was performed using ultracentrifugation (UC) as previously described (1). Seven different lipoprotein molecules including cholesterol (chol), triglycerides (tg), cholesterol ester (chole), free cholesterol (fchol), phospholipids (phosl), apolipoprotein A (apoA) and apolipoprotein B (apoB) were quantified in all or in some of the LP main fractions (VLDL, IDL, HDL, LDL) and/or in their subfractions (HDL2a, HDL2b, HDL3, LDL1, LDL2, LDL3, LDL4, LDL5, LDL6). Fractionation was done by sequential centrifugation of 3 mL EDTA plasma using an Optima L-80 XP ultracentrifuge with a fixed angle rotor type 50.4
Ti (Beckman Coulter, Inc., US). The UC process was initiated immediately after the fasting plasma sample was collected, and it was completed over a period of 8 days. A detailed description of the separation steps can be found in (1). Immediately after the fractionation step, all subfractions were frozen and stored at -80⁰C for later analysis.
Colorimetric and turbidimetric assays were performed on an ABX Pentra 400 analyzer (ABX Pentra; Horiba ABX, Montpellier, France) to determine the plasma, main class and subclass concentrations of total chol, tg, apoA and apoB (ABX Pentra; Horiba Medical, France). Free cholesterol and phoslp were determined using colorimetric and turbidimetric assays (MTI Diagnostics, Germany).
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2

Broiler Serum Biochemical Analysis

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Blood serum from 16 broilers at 42 d of age in total (eight per treatment and two per replicate pen) was collected as already stated above to examine selected haematological and biochemical parameters. In detail, aspartate aminotransferase (SGOT-AST) (IU/L), alanine aminotransferase (SGPT-ALT) (IU/L), blood urea nitrogen (BUN) (mg/dL), γ-glutamyltransferase (γ-GT) (IU/L), alkaline phosphatase (IU/L), cholesterol (mg/dL), total proteins (g/dL), and fractions of albumins (g/dL) and globulins (g/dL) were assessed using an automatic ABX Pentra 400 analyzer (Horiba-ABX, Montpellier, France).
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3

Diagnosing Hashimoto's Thyroiditis through Biochemical Analysis

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Venous blood samples were collected from fasting patients for biochemical analyses (TSH, fT3, fT4, TPOAbs, TgAbs). The diagnosis of Hashimoto’s thyroiditis (HT) was based on the presence of a hypoechogenic thyroid structure on US examination and elevated serum concentration of thyroid peroxidase antibodies (TPOAbs) and/or antibodies against thyroglobulin (TgAbs) [16 (link)]. HT was diagnosed on the basis of typical clinical symptoms (tiredness, weakness, dry skin, feeling cold, etc.), decreased fT3 and/or fT4 and decreased TSH and, additionally, the presence of anti-thyroid antibodies. All the tests were performed at the Department of Laboratory Medicine, Nicolaus Copernicus University, Collegium Medicum, Bydgoszcz, Poland using a Horiba ABX Pentra 400 analyzer (Horiba ABX, Montpelier, France).
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4

Kidney Function Monitoring Protocol

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All patients were scheduled to follow-up clinical and laboratory parameters at their respective district hospitals at 3-month intervals. All blood and urine samples were analyzed with ABX Pentra 400 analyzer (HORIBA ABX S.A.S., France) located at these hospitals. All biochemistry analyses were validated according to the standard protocol of Department of Medical Sciences, Ministry of Public Health, Thailand. Serum creatinine was measured by the enzymatic method. It was standardized with standard reference material (SRM 967) by commutability study every 6 months [17 (link)]. Two out of twelve subgroups of both patient groups were randomly selected and assigned to collect 24-h urine urea nitrogen and sodium.
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5

Detailed Blood Collection and Analysis Protocol

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A detailed description of blood collection and laboratory measurements has been previously published [10 (link)]. Routine laboratory measurements were performed in fresh serum (basic lipid profile [TC, TG, LDL-C], AST, ALT, CK). The remaining serum was aliquoted and stored at −80°C until assayed for hs-CRP, apoB, Lp(a). All measurements (except for CRP) were performed using the Horiba ABX Pentra 400 analyzer (Horiba ABX, Montpellier, France). LDL-C was measured directly. CRP was measured using the Alinity c analyzer (Abbott Laboratories, Chicago, IL, USA) with the Alinity c CRP Vario High Sensitivity assay for the quantitative, immunoturbidimetric determination of CRP with a limit of detection of 0.4 mg/L. Laboratory measurements were performed at the Department of Laboratory Medicine, Nicolaus Copernicus University, Collegium Medicum, Bydgoszcz, Poland.
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6

Comprehensive Metabolic Profiling in Fasted Subjects

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A protocol of laboratory investigation is summarized in Table 4. Prior to each hospital visit, all subjects will be instructed to have an 8-hour overnight fast. Plasma glucose, lipid profile, HbA1C, albumin, electrolytes, and urine analysis will be measured in the next morning. After centrifugation, plasma and urine samples will be centrifuged and transported at 4°C and analyzed within 24 hours at the laboratory Department of the District 2 Hospital. All blood chemistries will be measured with ABX Pentra 400 analyzer (HORIBA ABX S.A.S., France). Urine protein and creatinine will be measured with the pyrogallol red colorimetric method with the same analyzer. All biochemistry analyses will be validated according to the standard protocol of Department of Medical Sciences, Ministry of Public Health, Thailand. Serum creatinine will be measured by the enzymatic method. Serum creatinine measurement will be standardized with standard reference material (SRM 967) by commutability study at every 6 months [14 (link)].
Two out of twelve subgroups of both groups will be randomly selected and assigned to collect 24-hour urine. An aliquot of 24-hour urine of each sample will be sent for urine chemistry analysis protein, creatinine, urea, and sodium at Laboratory Department of Division of Nephrology, Chulalongkorn University, Bangkok, Thailand.
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7

Automated Mycophenolic Acid Quantification

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The ABX Pentra 400 analyzer (Horiba Ltd, Kyoto, Japan) is a compact bench top clinical biochemistry analyzer [20] (link). The configuration of the open channel was based on the Roche Total Mycophenolic Acid® assay package insert. Briefly, the analyzer added 3 µL of sample to 180 µL of R1 (cycle 1; a cycle every 12 s). After mixing and incubation for 5 min, 19 µL of R2 was added and mixed (cycle 26). At this point, bichromatic measurements (340/405 nm) were performed. The slope of the reaction rate is determined between cycles 49 and 62. The measuring temperature (37 °C) was controlled by an air bath. The assay was calibrated with 6-point calibration at 0, 1, 3, 5, 10 and 15 mg/L (Roche Total MPA Calibrators; Roche Diagnostics) using a logit/log4 calculation mode. All samples above 15 mg/L were manually diluted (1:3) with the diluent (equivalent to the 0 mg/L calibrator) from the Roche Total MPA Calibrators.
The assay was bidirectionally interfaced to the Laboratory Information System (LIS) using barcoded tubes. The reagents were stored in the integrated refrigerated reagent compartment (2–10 °C).
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8

Biomarker Analysis of Blood Samples

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Blood samples were collected using ethylenediaminetetraacetic acid (EDTA) tubes after overnight fasting. Samples were centrifuged (2000 rpm, 4 °C) for 20 min to separate plasma and were stored at −80 °C. Blood samples were shipped to the Interclinical Biochemical Laboratory at Sechenov University for biochemical analyses, including the measurements of circulating glucose, creatinine, uric acid, total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), plasma triglycerides (TGs), and thyroid hormones. Elevated glucose concentrations were categorized as >100 mg/dL [26 (link)]. Total cholesterol, LDL-C, VLDL-C, and TG were abnormal if >200 mg/dL, >100 mg/dL, >30 mg/dL, and >150 mg/dL, respectively [27 (link)]. Abnormal HDL-C was defined as <40 mg/dL in men and <50 mg/dL in women [27 (link)]. Abnormal serum creatinine was defined as >110 μmol/L for men and <45 or >90 μmol/L for women [28 ]. Abnormal uric acid was defined as >6.99 mg/dL and >5.6 mg/L in men and women, respectively [27 (link)]. Glucose, serum lipids, creatinine and uric acid were determined using an ABX Pentra 400 analyzer (Horiba ABX SAS, Montpellier, France). Extra plasma aliquots were shipped to the Laboratory of Pharmacokinetics and Metabolomics Analysis, Institute of Translational Medicine and Biotechnology at Sechenov University for metabolomics profiling.
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9

Multielemental Analysis of Livestock Samples

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Blood samples were collected in 9 ml serum collection tubes coated with clot activator (Vacuette®, Med-Kjemi AS, Norway). All samples were directly transported to the laboratory within a maximum of 120 min following sample collection without any prior chilling. Samples were analysed to determine the following parameters: iron (Fe), inorganic phosphate (P), copper (Cu), zinc (Zn), calcium (Ca), magnesium (Mg) and ceruloplasmin (Cp) levels. Levels of Fe and P were assessed by a colorimetric method (ABX Pentra 400 Analyzer, Horiba). Cu, Zn, Ca, Mg and Cp were determined by Atomic absorption spectrometry (AAnalyst 300 Perkin Elmer). Due to limited financial resources, blood sampling was restricted to Farms 2 and 3.
Tissue samples were stored at −20 °C until analysis. Inductively coupled plasma mass spectrometry (ICP-MS) was performed by SYNLAB.vet GmbH (Berlin, Germany) to determine Zn concentrations in liver and kidney samples.
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10

Biochemical and Cytokine Analysis of Post-Surgical Serum

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The blood samples were collected 30 h after surgery, under anesthesia, by cardiac puncture [13 (link)]. The serum samples obtained after centrifugation were immediately frozen at − 20 °C before being analyzed at the phenotyping department of the Toulouse Anexplo platform.
The biochemical determinations (ALP, ALT, AST, serum creatinine, serum lactates, and BUN) were carried out with the ABX Pentra 400 analyzer (Horiba ABX, Montpellier, France). Assessment of serum cytokine concentrations of IL-6 and IL-10 was performed using the Luminex technique (LXAMSM-02 2-plex Mouse Luminex Magnetic Assay, IL6, IL10, Bio-Techne, Abingdon, United Kingdom).
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