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Anti mouse pro cleaved il 1β

Manufactured by Cell Signaling Technology
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Anti mouse pro & cleaved IL-1β is a lab equipment product that detects both the pro and cleaved forms of the IL-1β protein in mouse samples. It is a tool for researchers to study the regulation and activation of this key inflammatory cytokine.

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Lab products found in correlation

Horseradish peroxidase conjugated secondary antibody, by Cell Signaling Technology (2 mentions) Protease inhibitor, by Cell Signaling Technology (2 mentions) Supersignal west pico chemiluminescent substrate, by Thermo Fisher Scientific (2 mentions) Pro caspase 1, by Abcam (2 mentions) 100 μl protein extraction reagent, by Thermo Fisher Scientific (2 mentions) Ag 20b 0042, by Adipogen (2 mentions)

Spelling variants of this product

IL-1β (253 citations) Anti-IL-1β (83 citations) Cleaved IL-1β (26 citations) Pro-IL-1β (21 citations) Mouse anti-IL-1β (18 citations) Anti-cleaved IL-1β (14 citations) Anti-pro-IL-1β (9 citations) Mouse anti-Pros (2 citations)

2 protocols using anti mouse pro cleaved il 1β

1

Western Blot Analysis of Inflammasome Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols
Purified DCs (1×106) were lysed with 100-μl protein extraction reagent (89900, Thermo Scientific) containing protease inhibitor (5872S, Cell Signaling). Equal amounts of protein (lysate) or supernatants were run on an SDS-PAGE and transferred onto nitrocellulose membranes following electro blotting. After blocking with 5% fat-free milk, the membranes were incubated at 4°C overnight with the following primary antibodies: anti mouse pro & cleaved IL-1β (Cell Signaling, 12507), pro-caspase-1 (Abcam, ab108362), Caspase-p20 & 10 (Adipogen-AG-20B-0042) as per manufacturer's instructions. The membranes were then washed was incubated with Horseradish peroxidase–conjugated secondary antibody (Cell Signaling). Proteins were visualized with SuperSignal West Pico chemiluminescent substrate (34078, Thermo Scientific).
Ravindran R., Loebbermann J., Nakaya H.I., Khan N., Ma H., Gama L., Machiah D.K., Lawson B., Hakimpour P., Wang Y.C., Li S., Sharma P., Kaufman R.J., Martinez J, & Pulendran B. (2016). The amino acid sensor GCN2 controls gut inflammation by inhibiting inflammasome activation. Nature, 531(7595), 523-527.
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2

Western Blot Analysis of Inflammasome Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols
Purified DCs (1×106) were lysed with 100-μl protein extraction reagent (89900, Thermo Scientific) containing protease inhibitor (5872S, Cell Signaling). Equal amounts of protein (lysate) or supernatants were run on an SDS-PAGE and transferred onto nitrocellulose membranes following electro blotting. After blocking with 5% fat-free milk, the membranes were incubated at 4°C overnight with the following primary antibodies: anti mouse pro & cleaved IL-1β (Cell Signaling, 12507), pro-caspase-1 (Abcam, ab108362), Caspase-p20 & 10 (Adipogen-AG-20B-0042) as per manufacturer's instructions. The membranes were then washed was incubated with Horseradish peroxidase–conjugated secondary antibody (Cell Signaling). Proteins were visualized with SuperSignal West Pico chemiluminescent substrate (34078, Thermo Scientific).
Ravindran R., Loebbermann J., Nakaya H.I., Khan N., Ma H., Gama L., Machiah D.K., Lawson B., Hakimpour P., Wang Y.C., Li S., Sharma P., Kaufman R.J., Martinez J, & Pulendran B. (2016). The amino acid sensor GCN2 controls gut inflammation by inhibiting inflammasome activation. Nature, 531(7595), 523-527.
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