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5 protocols using resomer rg 752h

1

PLGA Polymer Labeling Protocol

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PLGA (Resomer RG 503H and Resomer RG 752H) was purchased from Sigma-Aldrich and used as received. ATTO 488–NHS ester and Atto 633 amine were purchased from ATTO-TEC GmbH (Germany) and used as stock solutions (10 mg/ml) in dimethyl sulfoxide (DMSO).
All other chemicals were purchased from Sigma-Aldrich, unless otherwise specified. All chemicals were used as received.
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2

Characterization of PLGA Polymers

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Three different PLGAs were purchased from Sigma-Aldrich (Steinheim, Germany): PLGA (1) Resomer® RG 752 H, 75:25, acid terminated, 4,000–15,000 Da; PLGA (2) PLGA, 85:15, ester terminated, 50,000–75,000 DA; PLGA (3) Resomer® RG 756 S, 75:25, ester terminated, 76,000–115,000 Da. Subsequently, PLGAs are named with PLGA 1, PLGA 2, and PLGA 3. Ethyl acetate from Sigma-Aldrich (Steinheim, Germany) was used for preparing PLGA solutions.
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3

Synthesis and Characterization of PLGA-PEI Nanoparticles

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PLGA (Resomer® RG 752 H, lactide: glycolide 75:25, Mw 4000–15000), polyethylenimine (PEI, branched, Mw ~25,000), dichloromethane (DCM), dimethyl sulfoxide (DMSO), methanol, N,N′-dicyclohexylcarbodiimide (DCC), and N-hydroxysuccinimide (NHS) were purchased from Sigma-Aldrich (St. Louis, MS, USA). ICG was purchased from AdooQ (Irvine, CA, USA). Sodium hyaluronate was obtained from Lifecore Biomedical (Chaska, MN, USA). 4-(4,6-Dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride n-hydrate (DMT-MM) was purchased from Wako Pure Chemical Industries Ltd. (Tokyo, Japan). For cell culture, Dulbecco's modified Eagle's medium (DMEM, Gibco, Grand Island, NY, USA) and Iscove's modified Dulbecco's medium (IMDM, Gibco) supplemented with 10% (v/v) fetal bovine serum (FBS) and 1% antibiotic-antimycotic solution were used. DMEM, IMDM, FBS, and phosphate-buffered saline (PBS) were purchased from Lonza Walkersville (Basel, Switzerland). The cell counting kit-8 (CCK-8) was purchased from Dojindo Laboratories Co., Ltd. (Kumamoto, Japan). All reagents used were of analytical grade.
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Synthesis and Characterization of Hyaluronic Acid-Phospholipid Conjugates

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All the phospholipids, cholesterol (CHOL), PLGA 50:50 (Resomer® RG 502 H, 7–17 kDa), PLGA 75:25 (Resomer® RG 752 H, 4–15 kDa), PEG2000-PLGA 50:50 (PLGA 11.5 kDa), D-(+)-glucose, D-(+)-sucrose, D-(+)-trehalose, D-(+)-mannitol (purity minimum 95%) and solvents (analytical grade) were purchased from Merck (Milan, Italy). Sodium hyaluronate (HA) (4.8 kDa or 14.8 kDa) was purchased from Lifecore Biomedical (Chaska, MN, USA). The compound 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine (DPPE) was conjugated to 4.8 kDa or 14.8 kDa HA (HA4.8-DPPE or HA14.8-DPPE) using the method described by Arpicco et al. [30 (link)]. Filtered MilliQ® water (Millipore, Merck) was used. Solvents were evaporated using a rotating evaporator (Heidolph Laborota 400, Heidolph Instruments, Schwabach, Germany) equipped with a vacuum pump (Diaphragm Vacuum Pump DC-4).
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5

Encapsulation of Trans-Ferulic Acid in Biodegradable Polymers

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Trans-Ferulic acid, Resomer® R 202 H, acid terminated, Mw 10.000–18.000 (PLA), Resomer® RG 752 H, acid terminated, lactide:glycolide 75:25, Mw 4.000–15.000 (PLGA), and Tween® 80 were supplied by Merck (Milan, Italy). Ethanol (96% purity) was obtained from J.T.Baker (Deventer, The Netherlands). Acetone and dialysis membrane (molecular weight cut off (Mwco) 3000 Da, diameter 11.5 mm; Spectra/Por®) were purchased from VWR International PBI Srl (Milan, Italy). Hydroxypropyl-β-cyclodextrin was obtained from Roquette Freres (Lestrem, France). Deionized water was used for all the preparations.
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