Fresh resected human LUAD and matched adjacent noncancerous tissue were obtained after informed consent and approval by the institutional review board (16-1514, 16-107, and 12-245) at Memorial Sloan Kettering Cancer Center. Human LUAD cell lines, human lung fibroblast cells and a normal bronchial epithelial cell line (NL20) were obtained from American Type Culture Collection; Lenti-X 293T cells were purchased from Takara Bio. All cells were tested for mycoplasma. The primary antibodies used were BRMS1 (Abcam, ab134968), c-fos (Cell Signaling, #2250), c-Jun (Cell Signaling, #9165), V5-epitope (Abcam, ab15828), CEACAM6 (Santa Cruz, SC-59899), Src (Cell Signaling, #2109), p-Src Y416 (Cell Signaling, #59548), p-fos S32 (Cell Signaling, #5248), actin (Santa Cruz, sc-8432), p-Jun S63 (Cell Signaling, #2361), RAMP1 (Thermo Fisher, 10327-1-AP), and F5 (Thermo Fisher, PA5-103046). The reagents used—c-fos inhibitor T5224 (S8966) and Src inhibitor Saracatinib (AZD0530, S1006)—were purchased from Selleck Chemicals. Collagen Type IV (C6745), Collagen Type I solution (C3867), and TCN (T7760) were purchased from Millipore Sigma; puromycin (A1113803) and blasticidin (A1113903) were purchased from Thermo Fisher; CellTiter-Glo was obtained from Promega (G9241); and poly-HEMA solution was purchased from Sigma-Aldrich (P3932).
Src inhibitor saracatinib
Manufactured by Selleck Chemicals
Sourced in United States
Saracatinib is a Src inhibitor, a type of laboratory equipment used for inhibiting the Src protein in experimental research settings. It functions by directly binding and inhibiting the Src protein, which plays a role in various cellular processes. The core function of Saracatinib is to provide researchers with a tool to study the effects of Src inhibition in their experiments.
Automatically generated - may contain errors
Lab products found in correlation
Blasticidin, by Thermo Fisher Scientific (1 mentions)
Puromycin, by Thermo Fisher Scientific (1 mentions)
C fos, by Cell Signaling Technology (1 mentions)
P src y416, by Cell Signaling Technology (1 mentions)
Brms1, by Abcam (1 mentions)
V5 epitope, by Abcam (1 mentions)
Ceacam6, by Santa Cruz Biotechnology (1 mentions)
P jun s63, by Cell Signaling Technology (1 mentions)
Ramp1, by Thermo Fisher Scientific (1 mentions)
Lenti x 293t cells, by Takara Bio (1 mentions)
C jun, by Cell Signaling Technology (1 mentions)
Celltiter glo, by Promega (1 mentions)
Fibroblast growth factor (fgf), by Cell Signaling Technology (1 mentions)
Pdgf bb, by Thermo Fisher Scientific (1 mentions)
Hepatocyte growth factor (hgf), by Proteintech (1 mentions)
Pcmv backbones, by Thermo Fisher Scientific (1 mentions)
Epidermal growth factor (egf), by Cell Signaling Technology (1 mentions)
Insulin, by Merck Group (1 mentions)
Sodium orthovanadate, by Merck Group (1 mentions)
Quikchange site directed mutagenesis kit, by Agilent Technologies (1 mentions)
2 protocols using src inhibitor saracatinib
1
LUAD Tissue and Cell Line Protocol
2
Studying PIK3CA/p110α Mutations in Mammalian Cells
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EGF (cat# 8916) and FGF (cat# 61,977) were purchased from cell signaling technology (MA, USA). PDGF-BB (cat# 100-14B) was purchased from Peprotech (NJ, USA). HGF (cat# HZ-1084) was purchased from Proteintech (IL, USA). Insulin (cat# I9278) and Sodium orthovanadate (cat# 450,243) were obtained from Sigma (Merck, Germany). Src inhibitor Saracatinib (cat# S1006) was purchased from Selleck (TX, USA). pCMV backbones (Invitrogen) were used for gene expression in mammalian cells. PIK3CA/p110α was cloned into pCMV-3×FLAG vector. Src, Fyn, and Lyn were cloned into pCMV-3×Myc vector. Point mutations of Y317F, Y508F, or Y317F/Y508F on PIK3CA/p110α were generated with QuikChange Site-Directed Mutagenesis Kit (Agilent, cat #200,518). MLC2 and myr-AKT constructs were purchased from GeneCopoeia (Guangzhou, China). The primers using for constructs cloning were listed in Table S3 .
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