We sacrificed mice 24 hours after last MRI for analysis. We stained 6-μm paraffin embedded sections with either hematoxylin and eosin for histology or incubated sections overnight at 4°C with anti-Ki67 (Cell Signaling, Danvers, MA, Cat # 12202S, 1:200) or MAF (1:100, Invitrogen, Cat# PA523179). For fluorescence microscopy, we used Alexa-594 fluorescent conjugated anti-rabbit secondary antibody (1:400, Thermo Fisher Scientifics, Waltham, MA, Cat# A11037) and counterstained with bisbenzamide (DAPI) to identify the nuclei. Visualization methods were as described (6 (link)).
Alexa 594 fluorescent conjugated anti rabbit secondary antibody
Manufactured by Thermo Fisher Scientific
Alexa-594 fluorescent conjugated anti-rabbit secondary antibody is a laboratory reagent used for the detection and visualization of rabbit primary antibodies in various immunoassays, such as Western blotting, immunohistochemistry, and immunofluorescence. This antibody is labeled with the Alexa Fluor 594 fluorescent dye, which emits a red-orange fluorescent signal upon excitation.
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2 protocols using alexa 594 fluorescent conjugated anti rabbit secondary antibody
1
Histological Analysis of Tumor Markers
2
Histological Analysis of Tumor Markers
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We sacrificed mice 24 hours after last MRI for analysis. We stained 6-μm paraffin embedded sections with either hematoxylin and eosin for histology or incubated sections overnight at 4°C with anti-Ki67 (Cell Signaling, Danvers, MA, Cat # 12202S, 1:200) or MAF (1:100, Invitrogen, Cat# PA523179). For fluorescence microscopy, we used Alexa-594 fluorescent conjugated anti-rabbit secondary antibody (1:400, Thermo Fisher Scientifics, Waltham, MA, Cat# A11037) and counterstained with bisbenzamide (DAPI) to identify the nuclei. Visualization methods were as described (6 (link)).
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