Jnm eca 500 mhz nmr instrument

The following antibodies were used in this study: anti-β-catenin (Santa Cruz Biotechnology; Santa Cruz, CA, USA), anti-phospho-STAT3 (Cell Signaling Technology; Danvers, MA, USA), anti-STAT3 (Cell Signaling Technology), anti-Bcl-X_L, anti-phospho-Src (Cell Signaling Technology), anti-Src (Cell Signaling Technology), and anti-β-actin (Sigma-Aldrich; St. Louis, MO, USA). Dendrobine was purchased from Wuhan ChemFaces Biochemical (Hubei, China), and its purity (98%) and structure were confirmed using ¹H NMR analysis (JNM-ECA 500 MHz NMR instrument, JEOL Ltd., Tokyo, Japan).

The NMR experiments were performed using a JNM-ECA 500 MHz NMR instrument (JEOL Ltd., Tokyo, Japan). HRESIMS was carried out on a JMS-700 MStation Mass Spectrometer (JEOL Ltd., Tokyo, Japan). UV spectra were recorded on an Evolution 260 Bio UV–Visible spectrophotometer (Thermo Fischer Scientific, Waltham, MA, USA). Thin-layer chromatographic (TLC) analysis was performed on Kieselgel 60 F254 (Merck, Darmstadt, Germany) and Kieselgel 60 RP-18 F254S (Merck), with visualization performed under UV light (254 and 365 nm) and 10% (v/v) sulfuric acid spray followed by heating (200 °C, 2 min). YMC Gel ODS-A (12 nm, S-150 μm; YMC Co., Kyoto, Japan) was used for column chromatography (CC). Preparative HPLC was performed using a Gilson Preparative HPLC system (Gilson Inc., Middleton, WI, USA) equipped with YMC Pack Pro C18 (5 μm, 250 × 20 mm, YMC Co.). Analytical HPLC was performed using an Agilent 1100 series system (Agilent Technologies, Palo Alto, CA, USA) equipped with YMC-Triart C18 (5 μm, 250 × 4.6 mm, YMC Co.). A [⁶⁰Co] γ-irradiator (150 TBq capacity; AECL, Ottawa, Canada) was used for gamma irradiation. All other chemicals and solvents used in this study were of analytical grade.