Stable isotope tracers, consisting of 2H-thymidine, 15N-thymidine, 2H-water, and 2H-glucose, were obtained from Cambridge Isotope Laboratories, Inc. Paraformaldehyde and osmium tetroxide utilized for tissue fixation and Epon resin used for tissue/cell embedding were obtained from Electron Microscopy Sciences, Inc.
2h glucose
Manufactured by Cambridge Isotopes
2H-glucose is a stable isotope-labeled form of glucose. It contains deuterium (2H) atoms substituted for hydrogen atoms in the glucose molecule. This product is commonly used as a tracer in metabolic studies and research applications.
Automatically generated - may contain errors
Lab products found in correlation
Paraformaldehyde (pfa), by Electron Microscopy Sciences (1 mentions)
Osmium tetroxide, by Electron Microscopy Sciences (1 mentions)
2h water, by Cambridge Isotopes (1 mentions)
Epon resin, by Electron Microscopy Sciences (1 mentions)
15n thymidine, by Cambridge Isotopes (1 mentions)
13c glucose, by Cambridge Isotopes (1 mentions)
15nh4cl, by Cambridge Isotopes (1 mentions)
2 protocols using 2h glucose
1
Stable Isotope Tracing Protocol
2
Isotopic Labeling of SARS-CoV E Protein
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The expression and purification methods for the truncated SARS-CoV E construct corresponding to residues 8–65 (ETR) have been described previously [19 (link)]. This construct does not have cysteines, as these are not required for oligomerization [18 (link),19 (link),28 (link),38 (link)]. In the present work, M9 media was supplemented with an appropriate combination of 15NH4Cl, 13C-glucose, 2H-glucose, and 2H2O (Cambridge Isotope Laboratories) to produce15N-, 13C-, 15N/13C- and 15N/2H-labeled ETR samples. For preparation of fully deuterated 15N/2H-labeled samples, freshly transformed E. coli cells were doubly-selected in LB agar plates and media prepared with 30% and 60% 2H2O, successively, and later grown in M9 media prepared with 99.9% 2H2O [39 (link),40 (link)].
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