The SW620, SW480, HCT116, LOVO, and HT-29 human CRC cell lines as well as NCN460 normal colonic epithelial cells were obtained from Shanghai Genechem Co. Ltd. (Shanghai, China). The CRC cell lines were cultured in L-15 medium (Gibco, Carlsbad, USA), and NCN460 cell lines were grown in RPMI 1640 (Gibco BRL). The medium were supplemented with 10% fetal bovine serum (Gibco, 10099-14) and 1% penicillin-streptomycin (Gibco) and incubated at 37°C and 5% CO2.
Sw620
Manufactured by Genechem
Sourced in China
The SW620 is a laboratory equipment used for cell culture and analysis. It provides a controlled environment for the growth and maintenance of cells. The device is designed to maintain stable temperature, humidity, and gas levels to support optimal cell culture conditions.
Automatically generated - may contain errors
Lab products found in correlation
Hct116, by Genechem (8 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (5 mentions)
Sw480, by Genechem (4 mentions)
Rpmi 1640, by Thermo Fisher Scientific (3 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (3 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (2 mentions)
Caco 2, by Genechem (2 mentions)
Complete dmem f 12 medium, by American Type Culture Collection (2 mentions)
Hct116 p53, by Genechem (2 mentions)
Dmem f12, by American Type Culture Collection (2 mentions)
Fetal bovine serum (fbs), by ExCell Bio (2 mentions)
Sw620, by Thermo Fisher Scientific (2 mentions)
L 15 medium, by Thermo Fisher Scientific (1 mentions)
Leibovitz s l 15, by Thermo Fisher Scientific (1 mentions)
Caov3, by Thermo Fisher Scientific (1 mentions)
Hek293t, by Thermo Fisher Scientific (1 mentions)
Mccoy s 5a, by Thermo Fisher Scientific (1 mentions)
Caco 2, by Thermo Fisher Scientific (1 mentions)
Sw480, by Thermo Fisher Scientific (1 mentions)
Mcf 7, by Thermo Fisher Scientific (1 mentions)
11 protocols using sw620
1
Culturing Human Colorectal Cancer Cell Lines
2
Profiling Human Cancer Cell Lines
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The human colorectal HCT 116 (CVCL_0291), Caco-2(CVCL_0025), HT-29 (CVCL_0320), SW480 (CVCL_0546), SW620 (CVCL_0547), breast MCF-7(CVCL_0031), T-47D (CVCL_0553), MDA-MB-231(CVCL_0062), ovarian Caov-3(CVCL_0231), SK-OV-3(CVCL_0532) cancer cell lines and HEK293T(CVCL_0063) were obtained from the GeneChem Corporation (Shanghai, China). OVCAR-3(CVCL_0465) was obtained from the Nanjing KeyGen Biology (Nanjing, China). All human cell lines have been authenticated using STR profiling. OVCAR-3, Caco-2, MCF-7, Caov-3 and HEK293T cells were cultured in DMEM (Gibco, Carlsbad, CA, USA). T-47D and OVCAR-3 cells were cultured with RPMI 1640 (Gibco, Carlsbad, CA, USA). HCT 116 and HT-29 cells were cultured with McCoy’s 5a (Gibco, Carlsbad, CA, USA). SW480, SW620, and MDA-MB-231 cells were cultured with Leibovitz’s L-15 (Gibco, Carlsbad, CA, USA) medium containing 10% fetal bovine serum, 100 U/mL penicillin, and 100 mg/mL streptomycin. To inhibit NF-κB activities, BAY 117082 (Selleckchem, Houston, TX, USA) was used.
3
Culturing Human Colorectal Cancer Cell Lines
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The human colorectal cancer cell lines RKO, HCT116, HT-29, DLD-1, SW480, SW620 and LoVo were purchased from Shanghai GeneChem Co., Ltd., (Shanghai, China). The cells were routinely grown in RPMI-1640 (Invitrogen; Thermo Fisher Scientific, Inc.) supplemented with 10% fetal calf serum and 1% penicillin/streptomycin (Invitrogen; Thermo Fisher Scientific, Inc.) at 37°C in a humidified incubator with 5% CO2.
4
Culturing Human Colorectal Cancer Cell Lines
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Human colorectal cancer SW620, DLD cell lines were purchased from the Shanghai Genechem Co. Ltd (China). SW620 and DLD cell lines were cultured in Roswell Park Memorial Institute (RPMI) 1640 (Invitrogen; Thermo Fisher Scientific, Inc., Carlsbad, CA, USA) supplemented with 10% fetal bovine serum (FBS; Hangzhou Sijiqing Bio-Engineering Material Ltd. Co, Hangzhou, China), 100 U/mL penicillin, and 100 mg/mL streptomycin (Invitrogen; Thermo Fisher Scientific, Inc., Carlsbad, CA, USA) at 37°C in 5% CO2 atmosphere.
5
Colorectal Cancer Cell Culture
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Fresh-frozen carcinoma tissues and adjacent normal tissues from CRC patients were obtained from Liaoning Cancer Hospital (Shenyang, China). Written informed consent was obtained from patients and the study protocol was approved by the Ethics Committee on Human Investigation of the Liaoning Cancer Hospital. Clinical details of the participants are shown in Table S1.
Cell culture CRC cell lines (HCT116 p53+/+ , HCT116 p53-/-, SW620 and HT29) were purchased from GeneChem (Shanghai, China), the human normal colon epithelial cell line (FHC) was purchased from ATCC (Manassas, USA), the cervical cancer cell line (HeLa) and the breast cancer cell line (MCF-7) were purchased from the Chinese National Infrastructure of Cell Line Resource (NICR) (Beijing, China). HCT116, SW620 and HeLa cells were cultured in RPMI-1640 medium (HyClone, Logan, USA). HT29 and MCF7 cells were cultured in high-glucose Dulbecco's modi ed Eagle's medium (DMEM) (HyClone, USA).
Both media were supplemented with streptomycin (100 µg/ml), penicillin (100 units/ml) and 10% fetal bovine serum (FBS; ExCell Bio, China). FHC cells were cultured in complete DMEM: F-12 medium (ATCC, USA) according to the instructions. All cells were cultured at 37℃ in a humidi ed incubator containing 5% CO 2 .
Cell culture CRC cell lines (HCT116 p53+/+ , HCT116 p53-/-, SW620 and HT29) were purchased from GeneChem (Shanghai, China), the human normal colon epithelial cell line (FHC) was purchased from ATCC (Manassas, USA), the cervical cancer cell line (HeLa) and the breast cancer cell line (MCF-7) were purchased from the Chinese National Infrastructure of Cell Line Resource (NICR) (Beijing, China). HCT116, SW620 and HeLa cells were cultured in RPMI-1640 medium (HyClone, Logan, USA). HT29 and MCF7 cells were cultured in high-glucose Dulbecco's modi ed Eagle's medium (DMEM) (HyClone, USA).
Both media were supplemented with streptomycin (100 µg/ml), penicillin (100 units/ml) and 10% fetal bovine serum (FBS; ExCell Bio, China). FHC cells were cultured in complete DMEM: F-12 medium (ATCC, USA) according to the instructions. All cells were cultured at 37℃ in a humidi ed incubator containing 5% CO 2 .
6
Cell Line Sourcing and Culture Conditions
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FHC was purchased from the American Type Culture Collection (ATCC, Manassas, Virginia), HIEC-6 was preserved by our laboratory for years, and CRC cell lines (SW620, HT29, and HCT116) were purchased from GeneChem (Shanghai, China). MCF7 and NCI-H1299 were purchased from the Chinese National Infrastructure of Cell Line Resource (NICR; Beijing, China). The culture conditions of all the cells were the same as those described in previously published study [23 (link), 54 (link), 55 (link)].
7
Cell Cultivation and Maintenance for Cancer Research
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HCT116, SW620, and HT29 cells were purchased from GeneChem (China) and FHC cells were obtained from ATCC (USA). HCT116 and SW620 cells were cultured in RPMI-1640 medium and HT29 cells were cultured in Dulbecco's Modified Eagle Medium (HyClone, USA). Both of the mediums were supplemented with 10% fetal bovine serum (ExCell Bio, China) and 1% penicillin-streptomycin (HyClone, USA). FHC was cultured in DMEM: F-12 (ATCC, USA), supplemented with 10 mM HEPES (at a final concentration of 25 mM), 10 ng/mL cholera toxin, 0.005 mg/mL insulin, 0.005 mg/mL transferrin, 100 ng/mL hydrocortisone, 20 ng/mL human recombinant EGF (Thermo Fisher PHG0311), 10% fetal bovine serum (GIBCO), and 1% penicillin-streptomycin. All cells were incubated at 37°C in a humidified atmosphere containing 5% CO2.
8
Culturing Human Colon Cell Lines
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Human colon cancer cell lines (LOVO, HT29, HCT116, and SW620) and the human colonic epithelial cell line (NCM460) were purchased from GeneChem (Shanghai, China). Cells were cultured in Dulbecco's modified Eagle's medium (DMEM) (Gibco, Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10% fetal bovine serum (FBS; Gibco, Thermo Fisher Scientific) and 1% penicillin and streptomycin (Solarbio, Beijing, China), and the cells were maintained in a 37°C incubator containing 5% CO2. The medium was replaced every 24–48 h according to the condition of the medium. Cell morphology and density were observed under an inverted microscope, and 0.25% trypsin (Gibco, Thermo Fisher Scientific) was used to digest cells for passaging when they reached 80% confluence.
9
Cell Culture Conditions for Colorectal Cancer
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HCT116, SW620, and HT29 cell lines were purchased from GeneChem (Shanghai, China) and human normal colon epithelial cells (FHC) cell line was obtained from the American Type Culture Collection (ATCC, Manassas, Virginia). Both HCT116 and SW620 were cultured in RPMI-1640 medium (HyClone; Logan, Utah); HT29 was cultured in Dulbecco’s Modified Eagle Medium (DMEM)/HIGH GLUCOSE (HyClone); FHC was cultured in DMEM: F-12 (ATCC, Manassas). HCT116, SW620, and HT29 culture mediums were supplemented with 10% fetal bovine serum (ExCell Bio; Shanghai, China), and FHC culture medium was supplemented with 10% fetal bovine serum (Gibco, Carlsbad, California). All cells were incubated in a humidified atmosphere containing 5% CO2 at 37°C.
10
Colorectal Cancer Cell Culture
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Fresh-frozen carcinoma tissues and adjacent normal tissues from CRC patients were obtained from Liaoning Cancer Hospital (Shenyang, China). Written informed consent was obtained from patients and the study protocol was approved by the Ethics Committee on Human Investigation of the Liaoning Cancer Hospital. Clinical details of the participants are shown in Table S1.
Cell culture CRC cell lines (HCT116 p53+/+ , HCT116 p53-/-, SW620 and HT29) were purchased from GeneChem (Shanghai, China), the human normal colon epithelial cell line (FHC) was purchased from ATCC (Manassas, USA), the cervical cancer cell line (HeLa) and the breast cancer cell line (MCF-7) were purchased from the Chinese National Infrastructure of Cell Line Resource (NICR) (Beijing, China). HCT116, SW620 and HeLa cells were cultured in RPMI-1640 medium (HyClone, Logan, USA). HT29 and MCF7 cells were cultured in high-glucose Dulbecco's modi ed Eagle's medium (DMEM) (HyClone, USA).
Both media were supplemented with streptomycin (100 µg/ml), penicillin (100 units/ml) and 10% fetal bovine serum (FBS; ExCell Bio, China). FHC cells were cultured in complete DMEM: F-12 medium (ATCC, USA) according to the instructions. All cells were cultured at 37℃ in a humidi ed incubator containing 5% CO 2 .
Cell culture CRC cell lines (HCT116 p53+/+ , HCT116 p53-/-, SW620 and HT29) were purchased from GeneChem (Shanghai, China), the human normal colon epithelial cell line (FHC) was purchased from ATCC (Manassas, USA), the cervical cancer cell line (HeLa) and the breast cancer cell line (MCF-7) were purchased from the Chinese National Infrastructure of Cell Line Resource (NICR) (Beijing, China). HCT116, SW620 and HeLa cells were cultured in RPMI-1640 medium (HyClone, Logan, USA). HT29 and MCF7 cells were cultured in high-glucose Dulbecco's modi ed Eagle's medium (DMEM) (HyClone, USA).
Both media were supplemented with streptomycin (100 µg/ml), penicillin (100 units/ml) and 10% fetal bovine serum (FBS; ExCell Bio, China). FHC cells were cultured in complete DMEM: F-12 medium (ATCC, USA) according to the instructions. All cells were cultured at 37℃ in a humidi ed incubator containing 5% CO 2 .
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