High performance liquid chromatography grade methanol

Gefitinib, nilotinib, pazopanib, vemurafenib, imatinib, axitinib, crizotinib, Z-sunitinib, and E-sunitinib were isolated by Waters® ACQUITY UPLC® I CLASS (Milford Waters, MA, USA) and quantified using SCIEX® TRIPLE QUAD^TM 5500(AB Sciex, CA, USA). Gefitinib, nilotinib, pazopanib, vemurafenib, imatinib, axitinib, crizotinib, Z-sunitinib, and e-sunitinib were purchased from Aladdin (Shanghai, China). N-desethyl sunitinib hydrochloride, gefitinib-D6, nilotinib-D3, pazopanib-[C13, D3] hydrochloride, vemurafenib-¹³C6, imatinib-D8, axitinib-[C13, D3], crizotinib-[¹³C2, D5], sunitinib-D4 and N-desethyl sunitinib-D5 hydrochloride were obtained from Shimatsu AlsaChim (Illkirch-Graffenstaden, France). High-performance liquid chromatography grade methanol and acetonitrile were purchased from Merck Millipore (Billerica, MA USA), formic acid from Roe Scientific (Newark, DE, USA) and DMSO from Aladdin (Shanghai China). Mass spectrometry-grade ammonium formate was purchased from Sigma-Aldrich (Darmstadt, Germany). Human blank plasma was collected from an abandoned blood sample of apparently healthy individualswhich were provided by the health examination center of Zhongshan Hospital Fudan University (Shanghai, China) (IRB_B2020-310).

N-hexanol (99.6%, Merck) was used as the internal standard for the gas chromatography-flame ionization detector (GC-FID) calibration analysis. Pure alpha-pinene (147524, 98% Aldrich), sabinene (275166, 99% Aldrich), beta-pinene (80609, analytical standard [Fluka]), p-cymene (30039, analytical standard [Fluka]), and chamazulene (91595, analytical standard [Fluka]) were used as the standards as the five important components of the A. absinthium essential oil. Two, 2-diphenyl-1-picrylhydrazyl (DPPH), Folin-Ciocalteu (FC) reagent, sodium carbonates anhydrous, gallic acid, and ascorbic acid were purchased from Sigma-Aldrich GmbH (Sternheim, Germany). High-performance liquid chromatography-grade methanol was supplied by Merck (Darmstadt, Germany).

Standard compounds including CAP, ciprofloxacin (CIP), levofloxacin (LEV), norfloxacin (NOR), azithromycin (AZM), cefuroxime (CXM), and cefoperazone (CFP) were purchased from the National Institute for Pharmaceutical and Biological Products of China (Beijing, China). The purity of all standard chemicals was >99.8%. The CAP antibody was purchased from GeneTex (Irvine, CA, USA). CAP succinate sodium was purchased from Efebio (Shanghai, China). High performance liquid chromatography-grade methanol was purchased from Merck KGaA (Darmstadt, Germany). CM5 chips, 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride, N-hydroxysuccinimide (NHS), ethanolamine, phosphate-buffered saline (PBS), and HBS-EP buffer were provided by GE Healthcare (Chicago, IL, USA). Dimethyl sulfoxide (DMSO) with a purity of >99.5% was purchased from Sigma-Aldrich (St. Louis, MO, USA). Purified water was obtained using a Milli-Q system (Millipore, Bedford, MA, USA).