Duplexed Silencer® Select siRNAs for EGFR (s565), HER2 (s613), HER3 (s4780), IGF1R (s7211), FGFR1 (s5165), InsulinR (s533802), AXL (s1846), HBEGF (s4353), JUN (s7658), DUSP4 (s1922), DUSP6 (s4379), and Bcl-xL (s4373) were purchased from Invitrogen (Carlsbad, CA, USA). The ALK-targeted siRNAs were purchased from Santa Cruz Biotechnology (Dallas, TX, USA). Cells were transfected with these siRNAs using Lipofectamine RNAi-MAX (Invitrogen) according to the manufacturer’s instructions. In all experiments, Silencer® Select siRNA for negative control #1 (Invitrogen) was used as the scrambled control. EGFR, JUN, DUSP4, DUSP6, and ALK knockdown were confirmed using western blot analysis. To perform a comprehensive analysis of siRNA screening, the Silencer® Select human kinase siRNA library V4 (Ambion, 4397918) was used according to the manufacturer’s instructions.
Silencer select human kinase sirna library v4
Manufactured by Thermo Fisher Scientific
The Silencer® Select human kinase siRNA library V4 is a collection of small interfering RNA (siRNA) molecules targeting human kinase genes. This library is designed to enable the study of gene function and signaling pathways involving kinases, which are important regulatory enzymes in various cellular processes.
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2 protocols using silencer select human kinase sirna library v4
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Comprehensive siRNA Screening for Kinases
2
Comprehensive siRNA Screening of Kinases
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Duplexed Silencer Select siRNAs against AXL [s1845(#1) and s1846(#2); Invitrogen, ATR (s536(#1) and s57272(#2); Invitrogen], and Bim (s195011; Invitrogen) were transfected into cells using Lipofectamine RNAi-MAX (Invitrogen) according to the manufacturer's instructions. The Silencer Select Negative Control No. 1 siRNA (siSCR, Invitrogen) was used as a scrambled control in all the experiments. AXL and ATR knockdowns were confirmed using Western blotting. Each sample was tested in triplicates in three independent assays. To perform a comprehensive siRNA screening analysis, the Silencer Select human kinase siRNA library V4 (Ambion, 4397918) was used according to the manufacturer's instructions.
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