Caspase-3 was immunohistochemically stained in paraffin-embedded tissue sections. Briefly, after deparaffinization and rehydration, antigen retrieval was performed and the Caspase-3 primary antibody (Novus Biologicals, Centennial, CO, USA) was applied. The avidin-biotin-peroxidase complex method was then carried out according to the manufacturer’s protocol (Novus Biologicals) using diaminobenzidine as a substrate and hematoxylin as counterstain. The percentage area of positively stained tissue was measured using Image J software in three images/samples.
Caspase 3 primary antibody
Manufactured by Novus Biologicals
Sourced in United States
The Caspase-3 primary antibody is a laboratory reagent used for the detection and analysis of the Caspase-3 protein. Caspase-3 is a crucial enzyme involved in the apoptosis (programmed cell death) process. This antibody can be used in various immunodetection techniques, such as Western blotting, immunohistochemistry, and flow cytometry, to identify and quantify the Caspase-3 protein in biological samples.
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Lab products found in correlation
2 protocols using caspase 3 primary antibody
1
Immunohistochemical Analysis of Caspase-3
2
Apoptosis Quantification in Testis
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Paraffin-embedded tissue sections of testis and epididymis were immunohistochemically stained using caspase-3 primary antibody (Novus Biologicals, USA) as a hallmark of apoptosis. Level of caspase-3 was quantified using avidin-biotin-peroxidase (Novus Biologicals, USA). All procedures were carried out according to the manufacturer protocol. For color development 3,3′-diaminobenzidine stain was used and hematoxylin was used as counterstain. The percent area of positively stained testicular tissue was measured by using Image J software in three fields/testis.
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