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Flag ha control

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The FLAG-HA control is a protein expression control that contains both the FLAG and HA epitope tags. It can be used as a positive control for experiments involving these common epitope tags.

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Lab products found in correlation

Lipofectamine rnaimax, by Thermo Fisher Scientific (2 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (2 mentions) Igf 1, by Thermo Fisher Scientific (2 mentions) Bkm120, by Selleck Chemicals (2 mentions) Lipofectamine 2000, by Addgene (2 mentions) Gdc 0941, by Selleck Chemicals (2 mentions) Eht1864, by Bio-Techne (2 mentions) Mk 2206, by Selleck Chemicals (2 mentions)

2 protocols using flag ha control

1

Stable and Transient Cell Line Generation

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Cell lines (ATCC) were grown in DMEM (Life Technologies) with 10% FBS (Hyclone). MCF-7 and T47D cells stably expressing shRNA targeting PTEN or mismatch control (shMM) were described previously [41 (link)]. Generation of other stable Cell lines is described in Supplementary Methods. In transient transfection experiments, cells were transfected with pcDNA plasmid encoding myc-PREX1-HA (from Atanasio Pandiella, CSIC-Universidad de Salamanca) or FLAG-HA control (Addgene) plus Lipofectamine 2000. Cells were transfected with siRNA targeting P-REX1 or AllStars Negative Control (Qiagen, Dharmacon) plus Lipofectamine RNAiMAX (Life Technologies). Cells were treated with IGF-1 (PeproTech), heregulin (HRG), BKM-120, GDC-0941, MK-2206 (Selleckchem), or EHT1864 (Tocris Bioscience) as indicated in figures.
Dillon L.M., Bean J.R., Yang W., Shee K., Symonds L.K., Balko J.M., McDonald W.H., Liu S., Gonzalez-Angulo A.M., Mills G.B., Arteaga C.L, & Miller T.W. (2014). P-REX1 creates a positive feedback loop to activate growth factor receptor, PI3K/AKT, and MEK/ERK signaling in breast cancer. Oncogene, 34(30), 3968-3976.
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2

Stable and Transient Cell Line Generation

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Cell lines (ATCC) were grown in DMEM (Life Technologies) with 10% FBS (Hyclone). MCF-7 and T47D cells stably expressing shRNA targeting PTEN or mismatch control (shMM) were described previously [41 (link)]. Generation of other stable Cell lines is described in Supplementary Methods. In transient transfection experiments, cells were transfected with pcDNA plasmid encoding myc-PREX1-HA (from Atanasio Pandiella, CSIC-Universidad de Salamanca) or FLAG-HA control (Addgene) plus Lipofectamine 2000. Cells were transfected with siRNA targeting P-REX1 or AllStars Negative Control (Qiagen, Dharmacon) plus Lipofectamine RNAiMAX (Life Technologies). Cells were treated with IGF-1 (PeproTech), heregulin (HRG), BKM-120, GDC-0941, MK-2206 (Selleckchem), or EHT1864 (Tocris Bioscience) as indicated in figures.
Dillon L.M., Bean J.R., Yang W., Shee K., Symonds L.K., Balko J.M., McDonald W.H., Liu S., Gonzalez-Angulo A.M., Mills G.B., Arteaga C.L, & Miller T.W. (2014). P-REX1 creates a positive feedback loop to activate growth factor receptor, PI3K/AKT, and MEK/ERK signaling in breast cancer. Oncogene, 34(30), 3968-3976.
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