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Mtor pathway antibody sampler kit

Manufactured by Cell Signaling Technology
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Sourced in United Kingdom, United States

The MTOR pathway antibody sampler kit contains a collection of primary antibodies targeting key components of the mTOR signaling pathway. This kit allows for the detection and analysis of mTOR-related proteins in various experimental systems.

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Lab products found in correlation

Anti sox2, by Cell Signaling Technology (1 mentions) Anti oct4, by Cell Signaling Technology (1 mentions) Anti igf2, by Abcam (1 mentions) Anti β catenin, by Santa Cruz Biotechnology (1 mentions) Anti nanog, by Cell Signaling Technology (1 mentions) Phospho akt pathway antibody sampler kit, by Cell Signaling Technology (1 mentions) Ab32360, by Abcam (1 mentions) Ab155686, by Abcam (1 mentions) Autophagy antibody sampler kit, by Cell Signaling Technology (1 mentions) Myc sc 788, by Santa Cruz Biotechnology (1 mentions) Anti p62, by Cell Signaling Technology (1 mentions) Anti p70s6k, by Cell Signaling Technology (1 mentions) Anti p p70s6k, by Cell Signaling Technology (1 mentions) Anti lc3, by Cell Signaling Technology (1 mentions)

3 protocols using mtor pathway antibody sampler kit

1

Pluripotency Marker Immunoblot

Cited 1 time
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Immunoblot was conducted as previously described15 (link) and the following primary antibodies were used in this study: anti-NANOG (#4903; Cell Signaling Technologies, Danvers, MA) anti-SOX2 (#3579; Cell Signaling Technologies), anti-OCT4 (#2840; Cell Signaling Technologies), anti-IGF2 (ab9574; Abcam, Cambridge, UK), phospho-AKT pathway antibody sampler kit (#9916; Cell Signaling Technologies), mTOR pathway antibody sampler kit (#9964; Cell Signaling Technologies), and anti-β-catenin (sc-47778; Santa Cruz Biotechnology, Dallas, TX).
Seol H.S., Akiyama Y., Lee S.E., Shimada S, & Jang S.J. (2020). Loss of miR-100 and miR-125b results in cancer stem cell properties through IGF2 upregulation in hepatocellular carcinoma. Scientific Reports, 10, 21412.
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2

Western Blot Analysis of Cellular Signaling

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The antibodies specific against JUN (9165S), JUND (5000S), P-JUN Ser63 (2361S), P-JUN Ser73 (9164S), AKT (9272S), p-AKT Ser473 (9271S), RELA (8242S), PIK3CA (4255S), PARP (9542S), GAPDH (5174S), NFE2L2 (12721S), MTOR Pathway Antibody Sampler Kit (9964S), Autophagy Antibody Sampler Kit (4445S), RPS6KB1 Substrates Antibody Sampler Kit (2903S) and ULK1 Antibody Sampler Kit (8359T) were purchased from Cell Signaling Technology. Antibodies to GFP (sc-390394), ETS1 (sc-55581), SP1 (sc-14027) and MYC (sc-788) were bought from Santa Cruz Biotechnology. Antibodies that are specific against NFKB1 (ab32360) and SQSTM1 (ab155686) were bought from Abcam. Antibodies against ACTB (A1978) and TUBA/α-tubulin (T6199) were bought from Sigma Aldrich Corporation. Western blotting was performed as described in our previous publication.73,74 (link)
Huang H., Zhu J., Li Y., Zhang L., Gu J., Xie Q., Jin H., Che X., Li J., Huang C., Chen L.C., Lyu J., Gao J, & Huang C. (2016). Upregulation of SQSTM1/p62 contributes to nickel-induced malignant transformation of human bronchial epithelial cells. Autophagy, 12(10), 1687-1703.
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3

Autophagy Pathway Antibody Analysis

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The following antibodies were used in the study: mTOR Pathway Antibody Sampler Kit, anti-P70S6K, anti-p-P70S6K, anti-LC3, anti-P62 and secondary antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA). 3-MA was purchased from Sigma-Aldrich (St. Louis, MO, USA).
Chen J.F., Wu P., Xia R., Yang J., Huo X.Y., Gu D.Y., Tang C.J., De W, & Yang F. (2018). STAT3-induced lncRNA HAGLROS overexpression contributes to the malignant progression of gastric cancer cells via mTOR signal-mediated inhibition of autophagy. Molecular Cancer, 17, 6.
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