Formalin-fixed frozen livers were stained with Sirius Red and anti-α-SMA Ab (Abcam). Immunohistochemistry was performed using DAB staining (Vector), and counterstaining with Hematoxilin. The following antibodies were used for immunostaining: rabbit anti-mouse α-SMA (Abcam, Cambridge, MA), rabbit anti-mouse Desmin (Thermo Fisher Scientific, Fremont, CA), rat anti-mouse F4/80 (eBioscience, San Diego, CA), or rabbit anti-mouse 4-HNE (Alpha Diagnostic Intl Inc., San Antonio, TX) antibodies, following incubation with Alexa Fluor ® secondary antibody. The images were taken using Nikon microscope, and analyzed by Image J.
Rabbit anti mouse desmin
Manufactured by Thermo Fisher Scientific
Rabbit anti-mouse Desmin is a primary antibody that recognizes the desmin protein, a type III intermediate filament found in muscle cells. This antibody is produced in rabbits and specifically binds to the mouse desmin protein. It can be used in various immunoassay techniques to detect and analyze the presence and distribution of desmin in mouse tissue samples.
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Lab products found in correlation
2 protocols using rabbit anti mouse desmin
1
Liver Fibrosis Immunohistochemistry Analysis
2
Liver Fibrosis Immunohistochemistry Analysis
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