Sephacryl s 300

Xanthine sodium salt, xanthine oxidase enzyme, cytochrome C from horse heart, nitroblue tetrazolium (NBT), dimethyl sulfoxide (DMSO), phenyl methosulfate (PMS), phenymethylsulfonyl fluoride (PMSF), 1,4 dithiothreitol (DTT), 1,10 phenanthroline, trypan blue dye, bovine serum albumin (BSA), blue dextran, crystal violet, Sephacryl S-300, DEAE cellulose, and kits of gel filtration molecular weight marker were product of Sigma Co. SDS molecular weight marker proteins were purchased from Pharmacia Co., DMEM, fetal bovine serum, HEPES buffer solution, RPMI-1640, gentamycin, and L-glutamine are purchased from Lonza, Belgium.

All the chemicals and reagents used were of the highest purity available. α-LA, HRP, β-ME, DEAE-cellulose, Sepharose-4B, Sephacryl S-300, Sephacryl S-200, Freund’s complete and incomplete adjuvants, bicinchoninic acid (BCA) protein estimation kit and Tween-20 were purchased from Sigma-Aldrich Chemicals (St Louis, MO, USA) and used as received. Anti-HSA and anti-HRP goat IgG were purchased from MP Biomedicals, Mumbai, India. DTNB was purchased from Himedia, Mumbai, India. HRP-conjugated goat anti-rabbit IgG was purchased from Bangalore Genei Pvt. Ltd. (Bangalore, India), Protein concentrators (Amicon Ultra-15 centrifugal filter units) were purchased from Merck Millipore, India. Milk powder (Nestle Everyday, India), Baby feed (Nutricia Dexolac, India.) and raw bovine milk were purchased from the local grocery store. Whey was isolated from raw bovine milk by acid precipitation of casein. SDS and other reagents used were of analytical grade.

Phosphatydylcholine, Triton X-100, phenol red, Dithiothreitol (DTT), Phenyl methyl sulfonyl fluoride (PMSF), 1,10 Phenanthroline, bovine serum albumin (BSA), Diethylaminoethyl cellulose (DEAE-Cellulose), marker proteins, Sephacryl S-300, and thromboplastin were from Sigma Chemical (St. Louis, USA). The other chemicals were of analytical grade. Human blood samples were obtained from the laboratory of medical center hospital of our institute.