Thc oh

Reference standards for THC-d3, THC-OH, THC-OH-d3, THC-COOH-d9, CBD, CBD-d3, CBDA, CBN-d3, CBNA, CBG, CBGA, CBC, CBCA, CBL, THCV, THCVA, CBDV and CBDVA were purchased from Cerilliant (Round Rock, TX, USA), CBG-d9 and CBC-d9 from Cayman Chemical Co. (Ann Arbor, MI, USA) via LGC Standards (Wesel, Germany), CBN, THC and THC-COOH from LGC Standards and THCAA from THC Pharm (Frankfurt a. M., Germany). All standards were certified reference material and had a purity of at least 99.0%, except for THC-OH-d3, THCAA, CBC, CBC-d9, CBCA, CBL, THCVA, CBDV and CBDVA (97.7%), THC-d3 (96.7%), and CBG-d9 (95.0%). All chemicals were obtained from Carl Roth (Karlsruhe, Germany), except for methanol (from Fisher Scientific (Loughborough, UK)) and acetonitrile (from AppliChem (Darmstadt, Germany)). Chemicals used in LC-MS/MS measurement processes were LC-MS grade. All other chemicals were HPLC grade, except for acetone and dichloromethane, which had a purity of 99.8% and 99.5%, respectively. Blank human serum used for calibration was obtained from voluntary healthy blood donors via blood donation service and was tested to be drug-free.

THC, THC-COOH, THC-OH, THC-D3, THC-COOH-D3, and THC-OH-D9 were purchased from Cerilliant (Round Rock, TX, USA). A second set of THC, THC-OH, and THC-COOH was purchased from Lipomed (Cambridge, MA, USA) to be used for quality control samples. Water and acetonitrile (LC–MS-grade) were obtained from Millipore (Burlington, MA, USA). Dansyl chloride, sodium bicarbonate, sodium carbonate, acetic acid, and formic acid (LC–MS-grade) were obtained from Sigma-Aldrich (St. Louis, MO, USA). Captiva EMR-Lipid columns (1 mL, 40 mg) were purchased from Agilent Technologies (Santa Clara, CA, USA). Chromatography was performed with a Kinetex Phenyl Hexyl column (3.0 × 50 mm, 2.6 μm) purchased from Phenomenex Inc. (Torrance, CA, USA).