Rhodamine b isothiocyante rbitc

The chitosan (M_w: 140–190 kDa, degree of deacetylation: >85%, the viscosity: 200–400 mPa s) was purchased from Aladdin (Shanghai, China). High-viscosity sodium alginate (M_w: 20–50 kDa) was purchased from Bright Moon Seaweed Group (Qingdao, China). α-Amylase from Aspergillus oryzae was purchased from Aladdin (Shanghai, China). Dextran sodium sulfate (DSS) was supplied by MP Biomedicals (Irvine, CA). Cur and β-CD were purchased from Solarbio (Beijing, China). Male C57BL/6 mice (8-week old, 18–22 g) were provided by Pengyue Laboratory Animal Technology Co., Ltd. (Jinan, China) and preserved in an aseptic environment. Mouse interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), and interleukin-1β (IL-1β) ELISA kits were purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). Rhodamine B isothiocyante (RBITC) and fluorescein isothiocyanate (FITC) were purchased from Macklin (Shanghai, China).

PLGA (lactide: glycolide = 50:50, ester terminated, Mw = 38,000−54,000), Polyvinylalcohol (PVA, 86.5–89% hydrolyzed, viscosity 4.6–5.4 mPa·s) and resveratrol were purchased from Aladdin (Shanghai, China). Chitosan (molecular weight, 50,000–190,000; viscosity, 20–30 cP; and deacetylation 75%) and sodium alginate (low viscosity, 80,000–120,000; molecular weight, viscosity 2000 cP) were obtained from Sigma-Aldrich (St. Louis, MO, USA). Dextran sodium sulfate (DSS) was supplied by MP Biomedicals (Irvine, CA, USA). HE kits were purchased from Solarbio (Beijing, China). Male C57BL/6 mice (eight-week-old, 18–22 g) were provided by Pengyue Laboratory Animal Technology Co., Ltd. (Jinan, China), and preserved in an aseptic environment. Rhodamine B isothiocyante (RBITC) was purchased from Macklin (Shanghai, China). All other materials are analytical grade. Distilled water is used throughout the process.