NPSCs were trypsinised, collected and washed twice with phosphate-buffered saline (PBS). Then, the NPSCs were stained with the following conjugated antibodies: CD29-PE (Biolegend), CD90-FITC (Biolegend), CD44-FITC (Biolegend), CD45-FITC (Santa Cruz) and CD34-FITC (Santa Cruz). An isotype control antibody (Biolegend) was used for each examined antibody. The final antibody concentration was 1 mg/100 mL. After incubating in the dark for 30 min at 37 °C, the cells were washed three times with PBS. Samples were subjected to flow cytometry (Beckman, USA), and the percentage of positive staining was calculated relative to the isotype control staining.
Cd34 fitc
Manufactured by Santa Cruz Biotechnology
Sourced in United States, Japan
CD34-FITC is a fluorescently-labeled antibody that binds to the CD34 protein, which is expressed on the surface of hematopoietic stem and progenitor cells. This product can be used for the identification and enumeration of CD34-positive cells in flow cytometry applications.
Automatically generated - may contain errors
Lab products found in correlation
Reverse transcription kit, by Thermo Fisher Scientific (3 mentions)
Cd44 fitc, by BioLegend (2 mentions)
Cd90 fitc, by BioLegend (2 mentions)
Isotype control antibody, by BioLegend (1 mentions)
Cd45 fitc, by Santa Cruz Biotechnology (1 mentions)
Cd29 pe, by BioLegend (1 mentions)
Flow cytometry, by Beckman Coulter (1 mentions)
Hiscript 2 first strand cdna synthesis kit, by Vazyme (1 mentions)
Dmem f12, by Procell (1 mentions)
Actin, by Affinity Biosciences (1 mentions)
Aggrecan, by Affinity Biosciences (1 mentions)
Cyanine3 (cy3), by Affinity Biosciences (1 mentions)
Calcein am pi double stain kit, by Beyotime (1 mentions)
Freezol reagent kit, by Vazyme (1 mentions)
4 6 diamidino 2 phenylindole dapi, by Biosharp (1 mentions)
Fitc phalloidin, by Solarbio (1 mentions)
Cell counting kit 8 (cck8), by Biosharp (1 mentions)
Fetal bovine serum (fbs), by Procell (1 mentions)
Reactive oxygen species assay kit, by Solarbio (1 mentions)
Sybr qpcr master mix, by Vazyme (1 mentions)
3 protocols using cd34 fitc
1
Phenotypic Characterization of NPSCs
2
Mesenchymal Stem Cell Characterization
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Neochlorogenic
acids were purchased from Selleck.
CD44-FITC, CD45-FITC, and CD90-FITC were purchased from Biolegend
(United States). CD34-FITC was purchased from Santa Cruz Biotechnology
(United States). Aggrecan, Nrf2, HO-1, Nqo-1, Actin, and Cy3 antibodies
were purchased from Affinity Biosciences (China).
Annexin V-fluorescein
isothiocyanate (FITC)/PI Apoptosis Detection Kit was purchased from
KeyGEN BioTECH (China). Reactive oxygen species assay kit and Mitochondrial
Membrane Potential Assay Kit with JC-1 and FITC Phalloidin were purchased
from Solarbio (China). GelMA hydrogel was purchased from Suzhou Yongqinquan
Intelligent Equipment Co., Ltd. Calcein-AM/PI Double Stain Kit was
purchased from Beyotime Biotechnology (China). 4′,6-Diamidino-2-phenylindole
(DAPI) and Cell Counting KIT 8 was purchased from Biosharp (China).
DMEM/F12 and fetal bovine serum were purchased from Procell (China).
Osteogenesis, adipogenesis, and chondrogenesis differentiation kits
were purchased from OriCell(United States). FreeZol reagent kit, Hiscript
II First Strand cDNA Synthesis kit, and SYBR qPCR Master Mix were
purchased from Vazyme Biotech (China).
acids were purchased from Selleck.
CD44-FITC, CD45-FITC, and CD90-FITC were purchased from Biolegend
(United States). CD34-FITC was purchased from Santa Cruz Biotechnology
(United States). Aggrecan, Nrf2, HO-1, Nqo-1, Actin, and Cy3 antibodies
were purchased from Affinity Biosciences (China).
Annexin V-fluorescein
isothiocyanate (FITC)/PI Apoptosis Detection Kit was purchased from
KeyGEN BioTECH (China). Reactive oxygen species assay kit and Mitochondrial
Membrane Potential Assay Kit with JC-1 and FITC Phalloidin were purchased
from Solarbio (China). GelMA hydrogel was purchased from Suzhou Yongqinquan
Intelligent Equipment Co., Ltd. Calcein-AM/PI Double Stain Kit was
purchased from Beyotime Biotechnology (China). 4′,6-Diamidino-2-phenylindole
(DAPI) and Cell Counting KIT 8 was purchased from Biosharp (China).
DMEM/F12 and fetal bovine serum were purchased from Procell (China).
Osteogenesis, adipogenesis, and chondrogenesis differentiation kits
were purchased from OriCell(United States). FreeZol reagent kit, Hiscript
II First Strand cDNA Synthesis kit, and SYBR qPCR Master Mix were
purchased from Vazyme Biotech (China).
3
Immunophenotyping and Functional Analysis of Cells
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DMEM-F12 (Gibco, Grand Island, CA, United States), RPMI-1640 (Gibco), fetal bovine serum (FBS) (Biowest, Loire Valley, France), gel preparation kit (SDS-PAGE) (Boster, Suzhou, China), reverse transcription kit (Thermo Scientific, Massachusetts, MN, United States), SYBR Green kit (TaKaRa, Japan), antibodies against CD34 (FITC) (Santa Cruz Biotechnology, Santa Cruz, CA, United States), CD29 (PE), CD45 (PE), CD90 (FITC), RT1A (PE), RT1B (FITC) (Biolegend, San Diego, CA, USA), CD4 (FITC), CD25 (PE), FoxP3 (PE-Cyanine5) (eBioscience Inc, San Diego, CA, United States), Heme Oxygenase 1 (HO-1) (Abcam, Cambridge, United Kingdom), and CXCR3 (Affinity Bioreagent, United States), YAC-1 (Chinese Academy of Medical Sciences, Beijing, China), AD-Hmox1 (Shanghai Genechem Co. Ltd, Shanghai, China), AD-fusion gene (CXCR3/HO-1) (Shanghai Genechem Co. Ltd), and interleukin (IL)-2, IL-6, IL-10, IL-17, IL-23, tumor necrosis factor (TNF)-β, interferon (IFN)-γ, transforming growth factor (TGF)-β, and diamine oxidase (DAO) enzyme-linked immunosorbent assay (ELISA) kits (R&D Systems, Minneapolis, MN, United States) were employed in this study, as was HRP polymer (Jinmai Gene, Tianjin, China).
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