Representative H&E‐stained glass slides containing intratumoral lesions were examined. A core (3 mm in size) was collected from the invasive tumor front of each representative paraffin block and transplanted into the recipient tumor microarray (TMA) blocks. Immunohistochemical staining was carried out using an automated immunostainer (Benchmark Ultra, Ventana Medical Systems Inc., Tucson, AZ, USA) with monoclonal antibodies: anti‐CD9 antibody at a dilution of 1:50 (ab2215, Abcam, Cambridge, MA, USA, anti‐CD63 antibody at a dilution of 1:500 (ab134045, Abcam, Cambridge, MA, USA), anti‐LC3A/B antibody at a dilution of 1:100 (ab128025, Abcam, Cambridge, MA, USA), anti‐P62 antibody at a dilution of 1:2000 (ab56416, Abcam, Cambridge, MA, USA), and anti‐ANXA1 antibody at a dilution of 1:100 (ab33061, Abcam, Cambridge, MA, USA).
Ab33061
Manufactured by Abcam
Sourced in United States, United Kingdom
Ab33061 is a mouse monoclonal antibody that recognizes the human Tyrosine protein kinase ABL1. It can be used in various applications such as Western Blotting, Immunohistochemistry, and Immunocytochemistry.
Automatically generated - may contain errors
Lab products found in correlation
Benchmark ultra, by Roche (1 mentions)
Ab56416, by Abcam (1 mentions)
Ab128025, by Abcam (1 mentions)
Ab134045, by Abcam (1 mentions)
Ab2215, by Abcam (1 mentions)
Protease inhibitor cocktail, by Nacalai Tesque (1 mentions)
Pvdf membrane, by Bio-Rad (1 mentions)
β actin antibody, by Santa Cruz Biotechnology (1 mentions)
2 protocols using ab33061
1
Immunohistochemical analysis of tumor microenvironment
2
Western Blotting Procedure for Protein Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Standard Western blotting was done as previously described [21 (link)]. Briefly, after homogenization of the samples described above, lysis buffer (50 mM Tris–HCl [pH7.6], 150 mM NaCl, 1% Nonidet P-40, 0.5% sodium deoxycholate, 0.1% SDS) with a protease inhibitor cocktail (Nacalai Tesque, Kyoto, Japan). The whole tissue/cell lysates were incubated on ice for 15 min and then centrifuged at 14,000 rpm for 30 min at 4°C. A Bradford ULTRA Total Protein Quantitation Kit (Novexin Ltd., Cambridge, UK) was performed to detect the protein concentrations of the supernatants. Equal amount of lysates were resolved by SDS/PAGE and transferred electrophoretically to PVDF membrane (Bio-Rad). The membranes were probed with specific antibodies and the immunoreactive proteins were detected by the enhanced chemiluminescene (ECL) kit (Santa Cruz Biotechnology). Rabbit anti-Annexin A1 (1:1,000; ab33061) was purchased from Abcam Company (Cambridge, UK). Antibodies to COX-2 (D5H5) and cyclin D1 (92G2) were purchased from Cell Signaling Technology (Beverly, MA) and β-actin antibody were from Santa Cruz Biotechnology (Santa Cruz, CA).
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