Goat anti rabbit igg h l hrp conjugate and goat anti mouse igg h l hrp conjugate

ERK1/2, Phospho-ERK1/2 (Thr202/Tyr204), Akt (pan), phospho-Akt (S473),PLC γ2, pPLC γ2(Tyr1217),BTK, Phospho-BTK (Tyr223),Lyn(5G2), cleaved PARP (Asp214), CD79a, phospho-CD79a (Tyr182), Syk,phospho-Syk (Tyr525/526), SHIP1 and phospho-SHIP1 (Tyr1020) antibodies were from Cell Signaling Technology (Beverly, MA). Anti-SRC family (phospho Y418)-Phospho-Lyn (Y396) was obtained from Abcam (Cambridge,UK).Purified anti-human actin antibody was obtained from MP Biomedicals (Illkirch,France). Goat anti Rabbit IgG (H+L)-HRP conjugate and Goat anti Mouse IgG (H+L)-HRP conjugate and Goat F(ab’)2 anti-human IgM or IgG were from Jackson Immunoresearch Laboratories, (West Grove, PA). Dynabeads Human T-Activator CD3/CD28 were obtained from Thermo Scientific (Rockford, IL). All antibodies utilized in the study were used in concentrations according to the manufacturer’s instructions. Ficoll-Paque PLUS from GE healthcare (Uppsala, Sweden), dimethyl sulfoxide (DMSO) from Merck (Darmstadt, Germany), RPMI, fetal calf serum (FCS), Dulbecco’s phosphate buffered saline (PBS), L-glutamine and penicillin-streptomycin were from Biological Industries (Beit-Haemek, Israel). BTK PROTACs RC-2, IR-2 and NC-1 (Supplementary Figure 1) were designed as previously described (25 (link)).

ERK1/2, phospho-ERK1/2 (Thr202/Tyr204), Akt (pan), phospho-Akt (S473), CD79b, CD79a, phospho-CD79a (Tyr182), SHP1, phospho-SHP1 (Tyr564), SHIP1, phospho-SHIP1 (Tyr1020), BTK, phospho-BTK(Tyr223), NF-kB p65, phospho–NF-kB p65 (ser536), IgM, Lyn, phospho-Lyn (Y507), LCK, CD86, and ZAP70 antibodies were from Cell Signaling Technology (Beverly, MA). Anti-SRC family (phospho-Y418)–phospho-Lyn (Y396), CD62L, and IgG antibodies were from Abcam (Cambridge, UK). Purified anti-human actin antibody was obtained from MP Biomedicals (Illkirch, France). Goat anti Rabbit IgG (H+L)–HRP conjugate and Goat anti Mouse IgG (H+L)–HRP conjugate, Goat F(ab′)2 anti-human IgM and Goat F(ab′)2 anti-human IgG were from Jackson Immunoresearch Laboratories (West Grove, PA). All antibodies utilized in the study were used in concentrations according to the manufacturer’s instructions. Ficoll-Paque PLUS from GE healthcare (Uppsala, Sweden), dimethyl sulfoxide (DMSO) from Merck (Darmstadt, Germany), RPMI, fetal calf serum (FCS), Dulbecco’s phosphate-buffered saline (PBS), L-glutamine, and penicillin-streptomycin from Biological Industries (Beit-Haemek, Israel) were utilized for cell cultures.