G8 high performance liquid chromatography

Venous blood samples were collected alongside the patient’s routine care requirements and sent to the Clinical Chemistry laboratory at University Hospital Birmingham Foundation Trust’s QEH for assay. The time of blood collection and testing by both capillary and venepuncture methods was recorded to ensure they were within 15 min of each other. In this study all patients received their venous blood sample collection first, followed by POCT.
The laboratory methods employed at the QEH Clinical Chemistry Laboratory were: the TOSOH G8 High Performance Liquid Chromatography (HPLC) for HbA1c measurement. Serum creatinine was measured by Alinity c enzymatic method. All blood collection methods being subject to external accreditation by UKAS against ISO15189 for quality assurance.
Finger-prick (capillary) testing was performed according to standardised operating procedures (SOP), in accordance with manufacturer’s guidelines and study protocols. The same brand and gauge lancet was used for each participant in order to draw blood.

Diabetes duration, management of diabetes, A1C, and comorbid diseases were collected as data for diabetes-related characteristics. Diabetes duration was classified into one year or less, 2–5 years, 6–10 years, 11–20 years, and over 20 years. Management of diabetes was grouped into no treatment, no medication, only insulin, only oral hypoglycemic agent (OHA), and both OHA and insulin. A1C was measured using the Tosoh G8 high-performance liquid chromatography (Tosoh, Tokyo, Japan) with venous blood samples. It was classified to less than 7% and 7% or more, indicating good glycemic control and poor glycemic control, respectively [31 (link)]. Comorbidities related to diabetes complications included hypertension, dyslipidemia, stroke, and coronary heart disease [32 (link)], and the presence of self-reported comorbid diseases was categorized as a yes or no.