Dna oligonucleotides

Mouse 3T3-L1 cell lines were purchased from American Type Culture Collection (ATCC), (Manassas, VA, USA). The cell culture reagents including Advanced Dulbecco’s modified Eagle’s medium (DMEM), GlutaMAX, Penicillin-Streptomycin (10,000 U/mL), and fetal bovine serum (FBS) were ordered from Gibco Life Technologies (Thermo Fisher Scientific, Waltham, MA, USA). The Oil Red O solution, 3-isobutyl-1-methylxanthine (IBMX), dexamethasone, Rosiglitazone, and insulin were obtained from Sigma-Aldrich (St. Louis, MO, USA). PureLink RNA purification kit by Ambion, cDNA synthesis kit, TaqMan Array Mouse GPCR Panel, advanced TaqMan PCR Master Mix, and SYBR Green PCR Master Mix were purchased from Applied Biosystem (Thermo Fisher Scientific, Waltham, MA, USA). DNA oligonucleotides were obtained from Macrogen. Antibodies were obtained from Thermo Fisher (Thermo Fisher Scientific, Waltham, MA, USA) and Cell Signaling Technology (Danvers, MA, USA).

Isoproterenol, SR-58611A, and3-isobutyl-1-methylxanthine(IBMX), were from Sigma–Aldrich (Hackettstown, NJ, USA); mouse anti-HA antibodies were from Biolegend (Berkley, CA, USA); Cy5-conjugated antimouse IgG were from Jackson Immuno-Research Laboratories (West Grove, PA, USA); DNA oligonucleotides were purchased from Macrogen (Seoul, South Korea); pLenti-C-mGFP vector was from Origene (Rockville, MD, USA); restriction enzymes were from Promega (Madison, WA, USA) and Phusion High-Fidelity DNA Polymerase Mix was from Thermofisher (Waltham, MA, USA).

For the spectroscopic studies, a synthetic single-stranded desalted oligoribonucleotide (pre-miR-150-PQS, ST1) was purchased from Invitrogen (Carlsbad, CA, USA) resuspended in nuclease-free water and stored at −20 °C until use. Concentration was determined by absorbance at 260 nm (NanoVue Plus, Biochrom, Holliston, MA, USA) using the molar extinction coefficient provided by the manufacturer. For all experiments, the oligoribonucleotide was diluted to the final experimental concentration in 10 mM Tris-HCl pH 7.5 containing varying KCl or LiCl concentrations, as indicated in each figure and folded by heating for 5 min at 95 °C and slowly cooling to 20 °C. For cloning and RT-qPCR experiments, DNA oligonucleotides were purchased from Macrogen (Seoul, Republic of Korea), resuspended in nuclease-free water, and stored at −20 °C until use. All sequences are shown in Supplementary Table S6.