UV-Vis absorption spectra were measured on a Perkin-Elmer Lambda 900 UV-Vis spectrometer. Methanol was used as the solvent to dissolve the nucleoside. Circular dichroism (CD) spectra were recorded with 5 µM RNA duplexes in 20 mM phosphate buffer (pH 7.0) on a JASCO-815 spectropolarimeter at 20°C over a wavelength range of 200–320 nm using a 1 cm path length quartz cuvette with a scanning speed of 100 nm/min, bandwidth of 1.0 nm and D. I. T of 1.0 s. Each spectrum was averaged from an accumulation of four scans and baseline-corrected against the buffer.
Lambda 900 uv vis spectrometer
Manufactured by PerkinElmer
Sourced in United States
The Lambda 900 UV-Vis spectrometer is a high-performance instrument designed for precise spectroscopic analysis. It measures the absorption or transmission of light in the ultraviolet and visible regions of the electromagnetic spectrum. The instrument is capable of providing accurate and reliable data for a wide range of applications.
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Lab products found in correlation
3 protocols using lambda 900 uv vis spectrometer
1
Nucleoside UV-Vis and CD Spectroscopy
2
Colloidal Nanocrystal Optical Characterization
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Ex situ optical measurements were performed on
diluted colloidal
dispersions in toluene. Samples were prepared by diluting the stock
solution of washed NCs with anhydrous toluene under nitrogen. Samples
were stored in closed quartz cuvettes. Absorption spectra were measured
on a double-beam PerkinElmer Lambda 900 UV–vis spectrometer.
Photoluminescence spectra and photoluminescence quantum yield (PLQY)
measurements were recorded on an Edinburgh Instruments FLS980 spectrofluorimeter
equipped with a 450 W xenon lamp as excitation source and double grating
monochromators.
diluted colloidal
dispersions in toluene. Samples were prepared by diluting the stock
solution of washed NCs with anhydrous toluene under nitrogen. Samples
were stored in closed quartz cuvettes. Absorption spectra were measured
on a double-beam PerkinElmer Lambda 900 UV–vis spectrometer.
Photoluminescence spectra and photoluminescence quantum yield (PLQY)
measurements were recorded on an Edinburgh Instruments FLS980 spectrofluorimeter
equipped with a 450 W xenon lamp as excitation source and double grating
monochromators.
3
Expression and Characterization of LH2-GUS Fusion Protein
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A single transconjugant colony was inoculated into M22+ liquid medium and grown under aerobic conditions. For the expression of LH2-GUS fusion protein, cell cultures were shifted from aerobic conditions to semi-aerobic conditions at an optical density of the sample measured at a wavelength of 600 nm (OD 600 ) of 0.8-1.0, and continuously induced by 1.0 mM isopropyl β-d-1-thiogalactopyranoside (IPTG) for 0, 6, 12, 18, 24, 30, and 36 h. The absorption spectrum was recorded on a Perkin Elmer lambda 900 UV/VIS spectrometer (USA).
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