The tumors were fixed in 4% (w/v) formaldehyde solution for 24 h followed by storage in 70% ethanol. The fixed tumors were processed, paraffin-embedded, and cut at 4 µm thick slices. The sections were stained for cleaved caspase-3 (for apoptosis), Ki-67 (for tumor proliferation), and CD31 (for angiogenesis). Both cleaved-caspase 3 (Cell Signaling Technology, Danvers, MA, USA) and Ki-67 clone SP-6 (Biocare, Concord, CA, USA) utilized a 1:100 antibody concentration followed by Envision Rabbit Horseradish Peroxidase (HRP) detection system (Dako, Carpenteria, CA, USA). CD31assay utilized a 1:1200 antibody concentration (Santa Cruz, Dallas, TX, USA) followed by Goat-on-Rodent (HRP) polymer system (Biocare, Concord, CA, USA). All sections were developed using DAB chromogen (Dako) and counterstained with Mayer’s Hematoxylin (Dako). The relative staining for each target was analyzed by ImageJ software (Bethesda, MD, USA) to determine the fraction of positive stain per unit tissue area.
Envision rabbit horseradish peroxidase hrp detection system
Manufactured by Agilent Technologies
Sourced in United States
The Envision Rabbit Horseradish Peroxidase (HRP) detection system is a laboratory equipment product from Agilent Technologies. It is designed to detect the presence and quantity of target analytes in biological samples using the horseradish peroxidase enzyme as a marker.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using envision rabbit horseradish peroxidase hrp detection system
1
Quantification of Tumor Biomarkers
2
Immunohistochemical Analysis of Lung Tumors
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Lung tumors from the therapeutic study were fixed in 4% w/v formaldehyde solution for 24 h and subsequently transferred to 70% (v/v) ethanol. Tissue samples were embedded in paraffin and sectioned into 4 μm-thick slices. The sections were deparaffinized and stained for cleaved caspase-3, Ki67, and CD31. Both cleaved-caspase 3 (Cell Signaling Technology, Danvers, MA, USA) and Ki-67 clone SP-6 (Biocare Medical, Concord, CA, USA) staining used a 1:100 antibody concentration followed by Envision Rabbit Horseradish Peroxidase (HRP) detection system (Dako, Carpinteria, CA, USA). CD31 assay used a 1:1200 antibody concentration (Santa Cruz Biotechnology, Santa Cruz, CA, USA) followed by Goat-on-Rodent HRP polymer system (Biocare Medical). All the sections were developed using DAB chromogen (Dako) and counterstained with Mayer’s Hematoxylin (Dako). The relative staining for each target was analyzed by Image J software (https://imagej.nih.gov/ij/download.html ) to determine the fraction of positive stain per unit tissue area.
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