Stable isotope labeled internal standard testosterone-2,3,4-13C3, were purchased from IsoSciences (King of Prussia, PA). Testosterone (T) pure standards was purchased from Steraloids (Newport, RI), and hydroxylamine hydrochloride, ultrapure methanol, and water (Chromasolv®) from SigmaAldrich (St. Louis, MO). Internal T-13C3 (0.5 ng) standard was added to mouse sera. An Agilent 6495 triple quadruple mass spectrometer (Agilent, Santa Clara, CA) equipped with a Jet Stream electrospray ion source (ESI, Agilent), a 1290 Infinity ultrahigh performance liquid chromatography system (Agilent) and MassHunter Workstation software (Agilent) was used to quantify testosterone in this study. Chromatographic separation of steroid oximes was conducted with a Chromolith C18 reverse phase column (50–2 mm) with a matching Chromolith guard column (5–2 mm). Testosterone oxime was introduced into ESI source and analyzed in the positive ion mode. Molecular ions for T (m/z 304.2) and for 13C3 internal standard T (m/z 307.2) were selected in the first quadrupole and quantified using product ion for T (m/z 124.1) and for internal standard T (m/z 127.1). The lower limit of quantification on column for T was 2.5 fg.
Testosterone pure standards
Manufactured by Steraloids
Testosterone (T) pure standards are laboratory reference materials used for calibration and quality control purposes in analytical testing. They provide a reliable and consistent source of the testosterone molecule to ensure accurate and precise measurement in various analytical techniques.
Automatically generated - may contain errors
2 protocols using testosterone pure standards
1
Quantitative Testosterone Assay by LC-MS/MS
2
Quantitative Testosterone Assay by LC-MS/MS
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Stable isotope labeled internal standard testosterone-2,3,4-13C3, were purchased from IsoSciences (King of Prussia, PA). Testosterone (T) pure standards was purchased from Steraloids (Newport, RI), and hydroxylamine hydrochloride, ultrapure methanol, and water (Chromasolv®) from SigmaAldrich (St. Louis, MO). Internal T-13C3 (0.5 ng) standard was added to mouse sera. An Agilent 6495 triple quadruple mass spectrometer (Agilent, Santa Clara, CA) equipped with a Jet Stream electrospray ion source (ESI, Agilent), a 1290 Infinity ultrahigh performance liquid chromatography system (Agilent) and MassHunter Workstation software (Agilent) was used to quantify testosterone in this study. Chromatographic separation of steroid oximes was conducted with a Chromolith C18 reverse phase column (50–2 mm) with a matching Chromolith guard column (5–2 mm). Testosterone oxime was introduced into ESI source and analyzed in the positive ion mode. Molecular ions for T (m/z 304.2) and for 13C3 internal standard T (m/z 307.2) were selected in the first quadrupole and quantified using product ion for T (m/z 124.1) and for internal standard T (m/z 127.1). The lower limit of quantification on column for T was 2.5 fg.
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