Anti pcofilin 1

Chemicals used were: bortezomib (LC Laboratories), Propidium Iodide and N-acetylcysteine (Sigma Aldrich). Primary antibodies used were: anti-Gas2^{36 (link)} anti-actin (Sigma/Aldrich) anti-USP1^{37 (link)} anti-USP33 (Millipore) anti-AKT (Cell Signaling) anti-UCH-L5 (Abcam), anti-USP18^{38 (link)} anti-HDAC4^{39 (link)} anti-MEF2D (BD Bioscience), anti-GRP78, anti-USP14, anti-Cofilin-1, anti-pCofilin-1 (Santa Cruz Biotechnologies).

The inhibitor of ER calcium-ATPase thapsigargin was purchased from Sigma-Aldrich (St. Louis, MO, USA). Dulbecco's Modified Essential Medium (DMEM), Fetal Bovine Serum (FBS), and F-actin probes of Rhodamine Phalloidin R415 were purchased from Gibco Invitrogen Corporation (Carlsbad, CA, USA). The Hoechst kit was from Beyotime Biotechnology Co. (Haimen, Jiangsu, China). The following antibodies, anti-p-Cofilin-1, anti-Cofilin-1, and anti-RhoA were from Santa Cruz Biotechnology (Santa Cruz, CA, USA). The p-Paxillin (Tyr118) and pS6 (Ser240/244) antibodies were from Cell Signaling Technology (Danvers, MA, USA) and anti-GAPDH was from Millipore (Billerica, MA, USA). Other chemicals were of the highest purity available.