The cell culture medium was performed. The preparation of 1 l of MEM supplemented with 10% FBS required 9.5 g MEM (GE Healthcare, USA), 2.2 g NaHCO3 (Merck, Germany), 100 mL fetal bovine serum (Gibco, New Zealand) and 900 mL deionized water. The MEM was gently mixed in 1000 mL beaker and steriled filtered through 0.22 μm filter upper cups (Jet Biofil, China) into 1000 mL duran bottle (Duran, Germany). The culture medium was kept at 4 °C, and warmed at 37 °C before use. The preparation of 1 l of DMEM supplemented with 10% FBS required 13.4 g DMEM (GE Healthcare, USA), 3.7 g NaHCO3 (Merck, Germany), 100 mL fetal bovine serum (Gibco, New Zealand) and 900 mL deionized water. The DMEM was gently mixed in 1000 mL beaker and steriled filtered through 0.22 μm filter upper cups (Jet Biofil, China) into 1000 mL duran bottle (Duran, Germany). The culture medium was kept at 4 °C, and warmed at 37 °C before use.
Na Rangsee N., Yanatatsaneejit P., Pisitkun T., Somparn P., Jintaridth P, & Topanurak S. (2020). Host proteome linked to HPV E7-mediated specific gene hypermethylation in cancer pathways. Infectious Agents and Cancer, 15, 7.
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