Atg16l1

Manufactured by Thermo Fisher Scientific

Atg16L1 is a protein that plays a key role in the autophagy process, which is the degradation and recycling of cellular components. It acts as a scaffold, helping to assemble the autophagy machinery and regulate the formation of autophagosomes, which are the vesicles that engulf and transport cellular material to the lysosome for degradation. Atg16L1 is an important component of the autophagy pathway and is involved in various cellular processes, but a detailed factual and unbiased description of its intended use in lab equipment is not available.

Automatically generated - may contain errors

Lab products found in correlation

Galectin 3, by BD (2 mentions) Ubiquitin fk2, by Enzo Life Sciences (2 mentions) Beclin 1, by Santa Cruz Biotechnology (2 mentions) β actin, by Santa Cruz Biotechnology (1 mentions) Pam3csk4, by InvivoGen (1 mentions) Atg13, by Cell Signaling Technology (1 mentions) Ndp52, by Abcam (1 mentions) Bafilomycin a1, by Santa Cruz Biotechnology (1 mentions) β actin, by Abcam (1 mentions)

2 protocols using atg16l1

Autophagy and Lipid Signaling Pathway Protocols

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

The following rabbit antibodies were used: Atg16L1 (#PA1-18296 Thermo Scientific), beclin-1 (#sc-11427 Santa Cruz), and LC3 (#L7543 Sigma, #PM036 MBL). The following mouse antibodies were used: beta-actin (#sc-81178 Santa Cruz), galectin-3 (#556904 BD Pharmingen), and ubiquitin (FK2, #BML-PW8810 Enzo Life Sciences). Pam3CSK4 was purchased from Invivogen. The synthetic SL analog (2,3-dipalmitoyl-2′-sulfate-α-α′-d-trehalose) we used was a kind gift from Drs. Jacques Prandi and Martine Gilleron (Institute of Pharmacology and Structural Biology, Toulouse) [28 (link),29 (link)]. The DIMs were prepared as previously described [30 (link)].

Bah A., Sanicas M., Nigou J., Guilhot C., Astarie-Dequeker C, & Vergne I. (2020). The Lipid Virulence Factors of Mycobacterium tuberculosis Exert Multilayered Control over Autophagy-Related Pathways in Infected Human Macrophages. Cells, 9(3), 666.

+ Open protocol

+ Expand

Autophagy Regulation in Cell Lines

Check if the same lab product or an alternative is used in the 5 most similar protocols

Diphenyleneiodonium chloride (DPI), Dimethylsulfoxide (DMSO), and Earle's balanced salt solution (EBSS) were purchased from Sigma. Bafilomycin A1 was purchased from Santa cruz. The following rabbit antibodies were used: ULK1 (Cell Signaling), Atg13 (E1Y9V, Cell Signaling), Atg16L1 (Thermo Scientific, MBL), Beclin-1 (Santa Cruz), CD63 (Santa Cruz), LC3 (Sigma, MBL), ndp52 (Abcam). The following mouse antibodies were used: β-actin (Abcam, Santa Cruz), IgG1 K isotype (eBioscience), Galectin-3 (BD Pharmingen), p62 (BD Transduction Laboratories), TLR2 (Invivogen), ubiquitin (FK2, Enzo Life Sciences).

Bah A., Lacarrière C, & Vergne I. (2016). Autophagy-Related Proteins Target Ubiquitin-Free Mycobacterial Compartment to Promote Killing in Macrophages. Frontiers in Cellular and Infection Microbiology, 6, 53.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!