Dopamine hydrochloride

BP powder was purchased from Nanjing Xianfeng Nanomaterial Technology Co., LTD. N-methylpyrrolidone (NMP) was purchased from Shanghai Maclin Biochemical Technology Co., LTD. mPEG-NH₂ was purchased from Shanghai Yiyi Biotechnology Co., LTD. Pte and dopamine hydrochloride were procured from Shanghai McLean Biochemical Technology Co., LTD. Phosphate buffer solution (PBS) was purchased from Shenggong Bioengineering (Shanghai) Co., LTD. CY5.5-PEG-NH₂ was purchased from Guangzhou Carbonwater Technology Co., LTD. Saline was purchased from Sichuan Kelun Pharmaceutical Co., LTD. Triphenyltetrazolium chloride (TTC) was acquired from Beijing Solaibao Technology Co., LTD. Beijing Langeco Technology Co., LTD supplied 4% paraformaldehyde. DMEM, CCK8 assay kit, and H&E staining kit were respectively purchased from Gibco, Invigentech, and Beijing Solaibao Technology Co., LTD.

Chloroauric acid tetrahydrate, sodium citrate, ferric chloride, anhydrous sodium acetate polyethylene glycol, dopamine hydrochloride, boron sodium hydrogen, phenanthrene and pyrene were purchased from McLean Biochemical Technology Co. Ltd. (Shanghai, China). Ethylene glycol, absolute ethanol and acetic acid were purchased from Chengdu Colon Chemicals Co. LTD (Chengdu, China). Graphene Oxide Dispersion was purchased from Sinopharm Nanjing Xianfeng Nanomaterials Technology Co. LTD (Nanjing, China). Trihydroxymethyl aminomethane was purchased from Shanghai Yuanye Biotechnology Co. LTD (Shanghai, China) [34 (link)]. All the above reagents were of analytical grade and can be used without further purification. The ultrapure water used in all experiments was obtained from a purification system [13 (link)].

Melamine sponge (Suzhou Huanxi Electronic Materials Co., Ltd., Suzhou, China), nano-Fe₃O₄ (20nm 99.0% metals basis, Aladdin Reagent Co., Ltd., Shanghai, China), absolute ethanol (Tianjin Kaitong Chemical Reagent Co., Ltd., Tianjin, China), dopamine hydrochloride (Shanghai Maclean Biochemical Technology Co., Ltd., Shanghai, China), cyclohexane (AR, Tianjin Komeo Chemical Reagent Co., Ltd., Tianjin, China), PDMS (Polydimethylsiloxane, Dow Corning, Midland, MI, USA), curing agent (Dow Corning).

Sodium alginate (AR, SA), calcium chloride (AR), chitosan (AR), and tris–HCl solution (pH 6.8) were purchased from Aladdin Reagent Co., Ltd. (Shanghai, China). Dopamine hydrochloride (98%) was purchased from Macklin Reagent Co., Ltd., and whey protein (80%) from Shanghai Yuanye Co., Ltd. SeMNPV occlusion bodies (OBs) were provided by the Guangzhou Biological Control Station. The Magnetic Animal Tissue Genomic DNA Kit, Guangzhou Jianlun Biotechnology Co., Ltd; The SYBR Select Master Mix (2X) Quantitative PCR kit, Thermo Fisher Scientific. 2 × high-efficiency PCR mix (including dyes), GETEC; The Thin agarose gel recovery kit, Shanghai Jierui Biotechnology Co., Ltd; The PMD18-T Vector Cloning Kit, TakaRa. Plasmid Mini Preparation Kit (Spin Column Type), Shanghai Jierui Biotechnology Co., Ltd.

Rongalite, dopamine hydrochloride and resorcinol were purchased from Macklin Company (Shanghai, China). Formaldehyde solution (37–40%) was obtained from J &K Scientific (Beijing, China). Glucose, sucrose, glutamate, prolamin and Ca²⁺ were supplied by Aladdin Industrial Corporation (Shanghai, China). Sodium carbonate, sodium hydroxide and hydrochloric acid were purchased from Sinopharm Chemical Reagent Co., Ltd. (Beijing, China). All the reagents were analytical grade.
Sterile syringe filters (0.45 μm) were obtained from Merck (Merck Millipore, Burlington, MA, USA). White opaque microplates were supplied by Corning Ltd. (Corning Life Science, Hartford, NY, USA). Ultra-pure water was used in all aqueous solution and was produced by Millipore purification system (MilliQ gradient A10, Millipore, Bedford, MA, USA). Fluorescence excitation and emission spectra, absorption spectra, optical density, and fluorescence intensity were measured using a microplate reader (SpectraMax M2e, Molecular Devices, San Jose, CA, USA). The pH values were measured by a pH meter supplied by Thermo Fisher Scientific Inc. (Orion 4 Star, Beverly, MA, USA). The mass spectrum analysis was performed on the electrospray ionization (ESI)-TRIPLE QUAD 5500 (AB SCIEX, Framingham, MA, USA.) in the negative ion mode.

PLA and GS were purchased from Sigma-Aldrich. Chitosan (CS, 95% deacetylated powder) and dopamine hydrochloride were provided by Macklin Biochemical Co., Ltd. Chlorosulfonic acid, dichloromethane (DCM) and N, N-dimethylformamide (DMF) were purchased from Aladdin Reagent Co. (Shanghai, China).