The breast cells were lysed with RIPA extraction reagent (Beyotime, China) supplemented with protease inhibitors (Sigma-Aldrich, USA). Total protein was separated using 10–12.5% sodium dodecyl sulfate polyacrylamide gel electrophoresis and transferred to 0.45 μm PVDF membrane (Millipore, USA). Primary antibodies were ERα (Cell Signaling Technology, #8644), TRIM11 (Proteintech, 10851-1-AP), HA (Proteintech, 51064-2-AP), Myc (Proteintech, 60003-2-Ig), GAPDH (Proteintech, 60004-1-Ig) antibodies. Bands were visualized using an enhanced chemiluminescence (ECL) kit (Boster, China) and detected by ChemiDoc XRS + Imaging System (Bio-Rad).
Trim11
Manufactured by Proteintech
TRIM11 is a recombinant protein that belongs to the TRIM (Tripartite Motif) family of proteins. It contains an N-terminal RING finger domain, a B-box type 1 domain, a coiled-coil domain, and a C-terminal SPRY domain. TRIM11 is involved in various cellular processes, including protein regulation and modulation.
Automatically generated - may contain errors
Lab products found in correlation
Protease inhibitor, by Merck Group (3 mentions)
Gapdh, by Proteintech (3 mentions)
Chemidoc xrs imaging system, by Bio-Rad (2 mentions)
Ecl kit, by Boster Bio (2 mentions)
0.45 μm pvdf membrane, by Merck Group (2 mentions)
Ripa extraction reagent, by Beyotime (2 mentions)
Flag tag (flag), by Proteintech (2 mentions)
Nitrocellulose membrane, by GE Healthcare (1 mentions)
Ecl kit, by Meilun (1 mentions)
4 protocols using trim11
1
Western Blot Analysis of Breast Cell Proteins
2
Protein Expression Analysis by Western Blot
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The ATC cells were lysed with Radio Immunoprecipitation Assay (Meilun, China) supplemented with protease inhibitors (Sigma-Aldrich, USA). Total protein was separated using SDS-PAGE and transferred to nitrocellulose membranes (GE Healthcare). Primary antibodies were GAPDH (Proteintech, 60004-1-Ig), YAP (Proteintech, 13584-1-AP), TRIM11 (Proteintech, 10851-1-AP), Myc (Proteintech, 60003-2-Ig), HA (Proteintech, 51064-2-AP), Flag (Proteintech, 20543-1-AP), antibodies. The proteins of interest were visualized using an enhanced chemiluminescence (ECL) kit (Meilun, China).
3
Immunoprecipitation of ERα and TRIM11
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Total cell lysis of MCF7 were precleared with rabbit IgG for 2 h and then immunoprecipitated with ERα (Cell Signaling Technology, #8644) or TRIM11 (Proteintech, 10851-1-AP) antibody overnight, while rabbit IgG (Santa Cruz) was used as the negative control. The bounded protein was analyzed by Anti- ERα or Anti-TRIM11 antibody.
4
Anaplastic Thyroid Cancer Protein Analysis
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The anaplastic thyroid cancer cells were lysed with RIPA extraction reagent (Beyotime, China) supplemented with protease inhibitors (Sigma-Aldrich, USA). Total protein was separated using 10-12.5% sodium dodecyl sulfate polyacrylamide gel electrophoresis and transferred to 0.45 μm PVDF membrane (Millipore, USA). Primary antibodies were YAP (Proteintech, 13584-1-AP), TRIM11 (Proteintech, 10851-1-AP), HA (Proteintech, 51064-2-AP), Myc (Proteintech, 60003-2-Ig), Flag (Proteintech, 20543-1-AP), GAPDH (Proteintech, 60004-1-Ig) antibodies. Bands were visualized using an enhanced chemiluminescence (ECL) kit (Boster, China) and detected by ChemiDoc XRS+ Imaging System (Bio-Rad).
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