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Total cholesterol and cholesteryl ester colorimetric assay kit 2

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The Total Cholesterol and Cholesteryl Ester Colorimetric Assay Kit II is a lab equipment product that provides a colorimetric method for the quantitative determination of total cholesterol and cholesteryl ester levels in biological samples.

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Lab products found in correlation

Lipid extraction kit, by Abcam (1 mentions) Porcine insulin elisa kit, by Mercodia (1 mentions) Cc 105, by TOMY (1 mentions) Hydroxyproline assay kit, by Merck Group (1 mentions) Alt activity assay kit, by Cayman Chemical (1 mentions) Ast activity assay kit, by Merck Group (1 mentions) Quantikine elisa kit, by R&D Systems (1 mentions)

4 protocols using total cholesterol and cholesteryl ester colorimetric assay kit 2

1

Plasma and Liver Lipid Analysis

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Most of the tested plasma parameters were determined with an automatic biochemical analyzer (Indiko Clinical Chemistry Analyzer, Thermofisher). Insulin levels were assessed by Elisa (Mercodia Porcine Insulin ELISA kit)). After KOH and MgCl2 extraction, liver TG levels were measured using a commercially available kit (TRIGLYCERIDES, Thermo Fisher Diagnostics). After extraction using the lipid extraction kit from Biovision, liver total cholesterol levels were determined with the Total cholesterol and Cholesteryl Ester Colorimetric Assay Kit II (Biovision).
Duvivier V., Creusot S., Broux O., Helbert A., Lesage L., Moreau K., Lesueur N., Gerard L., Lemaitre K., Provost N., Hubert E.L., Baltauss T., Brzustowski A., De Preville N., Geronimi J., Adoux L., Letourneur F., Hammoutene A., Valla D., Paradis V, & Delerive P. (2021). Characterization and Pharmacological Validation of a Preclinical Model of NASH in Göttingen Minipigs. Journal of Clinical and Experimental Hepatology, 12(2), 293-305.
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2

Cholesterol Content Quantification

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Total cholesterol content was measured using Total Cholesterol and Cholesteryl Ester Colorimetric Assay Kit II (Biovision) as per the manufacturer’s protocol.
Kumari R., Deshmukh R.S, & Das S. (2019). Caspase-10 inhibits ATP-citrate lyase-mediated metabolic and epigenetic reprogramming to suppress tumorigenesis. Nature Communications, 10, 4255.
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3

Enzymatic Measurement of Liver Cholesterol

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Total cholesterol in the liver tissue was measured enzymatically using the Total Cholesterol and Cholesteryl Ester Colorimetric Assay Kit II (K623-100; BioVision). Tissue homogenate was obtained by dispersing 15 mg tissue in 600 μl CHCl3:IPA:NP-40 (7:11:0.1) at 2,200 rpm for 1 min, using a handheld disperser (PT 1300 D; Kinematica Inc.). The homogenate was then centrifuged for 5 min at 15,000g. Afterward, we transferred 200 μl of the liquid phase to a new tube, dried it with air-flow-in at 40°C for 10 min using a centrifugal concentrator (CC-105; TOMY Digital Biology), and dissolved the dried lipids with 400 μl Cholesterol Assay Buffer. Total cholesterol was measured in a colorimetric assay according to the manual.
Takeuchi F., Liang Y.Q., Shimizu-Furusawa H., Isono M., Ang M.Y., Mori K., Mori T., Kakazu E., Yoshio S, & Kato N. (2023). Gene-regulation modules in nonalcoholic fatty liver disease revealed by single-nucleus ATAC-seq. Life Science Alliance, 6(10), e202301988.
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4

Measuring Plasma PCSK9, Cholesterol, and Liver Parameters

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Plasma levels of human and mouse PCSK9 were measured using the human and mouse PCSK9 Quantikine ELISA Kits, respectively (R&D Systems, Minneapolis, MN). Plasma cholesterol was assessed using the Total Cholesterol and Cholesteryl Ester Colorimetric Assay Kit II (BioVision, Mountain View, CA). Plasma lipoproteins were separated in pooled samples from each group by size-exclusion chromatography, and cholesterol was quantified with a system of detection online as described. 29 (link) The plasma alanine and aspartate aminotransferase (ALT/AST) levels were determined using the ALT Activity Assay Kit (Cayman Chemical, Ann Arbor, MI) and AST Activity Assay Kit (Sigma-Aldrich), respectively. Liver lipids were extracted using the BUME (butanol/methanol) method 30 (link) and quantified using straight-phase high-performance liquid chromatography as described previously. 31 To measure hepatic collagen content, liver samples were homogenized in distilled water, hydrolyzed in 12 N HCl for 3 hours at 120°C, and analyzed using a Hydroxyproline Assay Kit (Sigma-Aldrich).
Cansby E., Magnusson E., Nuñez-Durán E., Amrutkar M., Pedrelli M., Parini P., Hoffmann J., Ståhlman M., Howell B.W., Marschall H.U., Borén J, & Mahlapuu M. (2018). STK25 Regulates Cardiovascular Disease Progression in a Mouse Model of Hypercholesterolemia. Arteriosclerosis, thrombosis, and vascular biology, 38(8).
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